Efficacy of Vaporized Hydrogen Peroxide Combined with Silver Ions against Multidrug-Resistant Gram-Negative and Gram-Positive Clinical Isolates

Abstract

Antimicrobial resistance (AMR) is a serious public health problem that results in high morbidity and mortality rates. In particular, multidrug-resistant (MDR) strains circulating in hospital settings pose a major threat as they are associated with serious nosocomial infections. Therefore, regular cleaning and disinfection procedures, usually using chemical disinfectants, must be implemented in these facilities. Hydrogen peroxide (HP)-based disinfectants have proven high microbicidal activity and several comparative advantages over conventional disinfectants. We assessed the in vitro biocidal activity of an 8% HP solution combined with 30 mg/L silver ions (HP + Ag) against MDR clinical isolates of Klebsiella pneumoniae (MDRKp) and Pseudomonas aeruginosa (MDRPa), and methicillin-resistant Staphylococcus aureus (MRSA). Accordingly, the in vitro antibacterial activity was determined using the macrodilution method, and the efficacy was determined for 30 min in terms of (1) activity on bacteria in suspension and (2) activity on surfaces using vaporized HP + Ag on a 20 cm2 stainless steel surface. A strong bactericidal effect of HP + Ag was observed against MDRKp, MDRPa, and MRSA strains, with minimum inhibitory concentrations and minimum bactericidal concentrations between 362.5 and 5800 mg/L. A strong effect was observed during the 30 min of HP + Ag exposure to the resistant clinical isolates, with over 4-Log10 reduction in CFUs. Regarding the efficacy of the disinfectant on surfaces, bacterial load reductions of >99% were observed. These results suggest that HP + Ag is potentially useful as an effective disinfectant for decontaminating surfaces in hospital settings suspected of contamination with MDR bacteria.

Humic acids alleviate dextran sulfate sodium-induced colitis by positively modulating gut microbiota

“…In addition, antibiotics have been exploited to promote animal growth and reduce production costs in livestock breeding. Microbial resistance caused by the misuse of antibiotics has become a serious problem globally (Rivera-Sánchez et al, 2022 ; Cui et al, 2023 ). Therefore, it is necessary to explore alternatives to antibiotics for treating colitis.…”

Prevention and control of hospital-acquired infections with multidrug-resistant organism: A review

“…In comparison to manual cleaning, an aHP device reduced aerobic colony counts in the environment by 98% after a 42-minute operation. Rivera-Sánchez et al [32] combined an 8% HP solution with 30 mg/L silver ions to disinfect stainless steel surfaces after MDRO contamination using a VHP device (which should actually be called an aHP device). Cobrado et al [33] discovered that reducing the cycle time of the aHP system from the standard hour to 15 minutes did not result in variations in the growth inhibition of strains on different materials.…”