Efficacy of various collection methods for oocyte retrieval in buffalo

Das, Gopal Krushna; Jain, Geeta; Solanki, V.S.; Tripathi, Vandana

doi:10.1016/s0093-691x(96)00319-6

Cited by 53 publications

(55 citation statements)

References 11 publications

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“…These results were in agreement with that reported by Das et al, 1996 andSamad et al, 1998, who reported mean recovery rates of 1.7 and 1.76 respectively in buffaloes. However, higher oocyte recovery rates 2.2, 2.65 and 3.12 in buffaloes were reported by Tasripoo and Kamonpatana (1997), Shahid et al, (2014) and Ruhil and Purohit (2016) respectively.…”

Section: Resultssupporting

confidence: 93%

“…The mean oocyte recovery rate obtained from the ovaries with CL (1.35) was significantly (P ≤ 0.05) lower than that obtained from the ovaries without CL (1.97). Similar trend was reported by Das et al, (1996) and Raza et al, (2001) recorded that the presence of a CL significantly reduces the number of ovarian follicles as well as the quality of oocytes in buffaloes. This is because follicular development is restricted, as lutein cells occupy most of the ovary (Kumar et al, 1997).…”

Section: Resultssupporting

confidence: 86%

See 1 more Smart Citation

Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries

Rajesh¹,

Swathi²,

Kumari³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 86%

Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries

Rajesh¹,

Swathi²,

Kumari³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…The Egyptian buffalo possesses a greater number of good quality oocytes (1.4 oocytes/ovary). Our results were higher than previously recorded in the Indian buffalo [13,15] and this difference may be due to the variation in breed. The low reproductive capacity of the buffalo in terms of the total number of follicles per ovary, the high percentage of atretic follicles and the low rate of oocyte recovery could be attributed as a whole to the low average number of primordial follicles per ovary when compared with cattle [14].…”

Section: Discussioncontrasting

confidence: 97%

“…A small number of authors have reported on the in vitro maturation and fertilization of buffalo oocytes [2,5,13,15]. In this study, the cleavage rate and yield of blastocysts of in vitro fertilized Egyptian buffalo oocytes, using CR1aa medium for IVM, IVF and IVC, were 81% and 40.2% when coincubated with spermatozoa from Bull 2, which has 60% motile sperm.…”

Section: Discussionmentioning

confidence: 68%

New Technique, Using a Portable CO2 Incubator, for the Production of In Vitro Fertilized Egyptian Buffalo Embryos.

Kandil

Abdoon

Murakami

et al. 1999

Journal of Reproduction and Development

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Abstract. Buffalo ovaries were collected from a slaughter house and cumulus oocyte complexes were aspirated from follicles 2-6 mm in diameter. A portable CO2 incubator and a CR1aa medium were used for in vitro production of embryos. In the first experiment, numbers of follicles and oocytes per ovary were counted, and classified according to the appearance of the cumulus cells. In the second experiment, in vitro fertilization and cultivation of buffalo oocytes matured in vitro were carried out using semen from three different bulls, which had different sperm motilities (Bull 1: 20%, Bull 2: 60% and Bull 3: 15%, respectively). In the third experiment, the cleavage and transferable embryos of fertilized oocytes from denuded oocytes, and oocytes with intact cumulus cell layers were compared. The percentages of good, fair and poor quality oocytes were 60.9%, 17.8% and 21.4%, respectively. The rates of cleavage and transferable embryos were significantly higher after incubation with semen from Bull 2 (81% and 40.2%) than those after incubation with semen from Bulls 1 (52% and 13.5%, p<0.05 and p<0.01) and 3 (15.8% and 0%, p<0.01). The rates of cleavage and transferable embryos were significantly higher (p<0.01) for oocytes with an intact cumulus than for oocytes without cumulus cells at fertilization.

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“…Other records found that the stage of oestrous cycle has no effect on bovine oocytes quality or its developmental potentials [14]. Moreover, the number of follicles and yield of oocytes per ovary were less from buffalo ovaries bearing a CL [3,13]. In addition, methods of oocyte retrieval are directly affecting the yield and quality of oocytes.…”

Section: Introductionmentioning

confidence: 99%

Factors affecting number of surface ovarian follicles and oocytes yield and quality in egyptian buffaloes

Abdoon

Kandil

2001

Reprod. Nutr. Dev.

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-Three experiments were conducted to evaluate factors affecting number of surface ovarian follicles and oocytes yield and quality in buffalo. In Experiment 1, ovaries (n = 126) were collected in pairs from slaughtered anoestrus, early pregnant and cyclic buffaloes. Ovarian follicles (1-3, 4-9 and ≥ 10 mm diameter) were counted, aspirated and oocytes were recovered and evaluated. In Experiment 2, ovaries were divided into 2 groups. Group 1, ovaries bearing a CL (n = 74) and Group 2 non-bearing CL (n = 74), ovarian follicles (2-8 mm) were counted, aspirated and oocytes evaluated. In Experiment 3, oocytes were recovered using aspiration or slicing methods. In all experiments, oocytes were classified into good, fair, poor and denuded. Results showed that the development of small and total ovarian follicles are continuous and independent in early pregnant or cyclic buffalo cows, however, it significantly decreased (P < 0.01) in the ovaries of anoestrus buffaloes. Number of medium and large size follicles was significantly increased (P < 0.01) in cyclic buffaloes on Days 10-16 and 17-22 of oestrous cycle, while large follicles was significantly decreased (P < 0.01) in the ovaries of pregnant buffaloes. A significantly higher (P < 0.01) percentage of poor and denuded oocytes were recovered from ovaries of anoestrus and pregnant buffalo. While, the highest (P < 0.01) percentage of good quality oocytes were recovered from ovaries of cyclic buffaloes on Days 1-3 and 10-16 of oestrous cycle, eliciting that the stage of oestrous cycle is affecting the quality of buffalo oocytes. In addition, the presence of a CL stimulates the development of a significantly higher (P < 0.01) number ovarian follicles which produced a significantly higher (P < 0.05) number of good quality oocytes. Slicing of buffalo ovaries produced a significantly higher number of fair, poor and denuded oocytes. In conclusion, number of ovarian follicles and yield and quality of oocytes were affected by the reproductive status, stage of the oestrous cycle, presence of a CL and the method of oocytes retrieval. buffalo / reproductive status / follicle / oocyte quality / CL / oocyte retrieval Reprod. Nutr. Dev. 41 (2001) [71][72][73][74][75][76][77] 71

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Efficacy of various collection methods for oocyte retrieval in buffalo

Cited by 53 publications

References 11 publications

Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries

Effect of Ovarian Status on Oocyte Quality and Recovery Rate Retrieved by Aspiration Method in Buffalo Ovaries

New Technique, Using a Portable CO2 Incubator, for the Production of In Vitro Fertilized Egyptian Buffalo Embryos.

Factors affecting number of surface ovarian follicles and oocytes yield and quality in egyptian buffaloes

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