Efficiency of a novel gametocide amidosulfuron on rapeseed (Brassica napus)

Abstract

The efficiency of a novel gametocide amidosulfuron, 1-(4, 6-dimethoxypyrimidin-2-yl) -3-mesyl (methyl) sulfamoylurea, was evaluated on rapeseed (Brassica napus L.). Double application of 0.09-0.12 g/ha amidosulfuron at the uni-nucleate stage of longest buds and 12 days later, made 97.4-100% plants male-sterile in seventeen out of 20 cultivars tested. The shrunken anthers could not release pollen or produced only dysfunctional pollen grains that could not be stained by aceto carmine. The treatment also caused 10-30% reduction on seed setting in comparison to the controls. The purity of hybrid seed from crosses of ÔC161 · HuayeÕ and ÔZhongshuang No.2 · HuayeÕ based on amidosulfuron was 99.8% and 100.0%, respectively. The results suggested that amidosulfuron is an efficient gametocide for B. napus.

The sulphonylurea herbicide monosulphuron ester sodium as a special male gametocide in Brassica napus L.

“…As a result, seed purity reached 95.5% in the ZS09 × SH11 cross. Concerning the level of male sterility, time efficiency, dosage range, and yield parameters, the efficiency of MES as gametocide is comparable with two previously reported chemicals, tribenuron-methyl and amidosulphuron in rapeseed (Yu et al 2006(Yu et al , 2009. MES is very cheap and does not pose any environmental hazard or health risk (Kou et al 2006;Wang et al 2008).…”

“…Our group previously identified that two acetolactate synthase inhibitor herbicides, tribenuron-methyl and amidosulphuron, can induce complete male sterility in rapeseed when applied twice at a dosage (Yu et al 2006(Yu et al , 2009. In this investigation, we reported that MES, also an acetolactate synthase inhibitor herbicide, induced complete male sterility in 48 out of 49 rapeseed accessions when applied at a concentration < 1%.…”

“…To our knowledge, only methyl arsenates, tribenuron-methyl, and amidosulphuron have been used to produce commercial hybrid seeds on a large scale (Guan & Stringam 1998;Yu et al 2006Yu et al , 2009Yu & He 2014). However, methyl arsenates are environmentally hazardous.…”

“…In rapeseed, several chemicals are used to induce male sterility (Guan et al 1981Guan 1995Guan , 2012Guan & Stringam 1998;Zhang et al 1999;Chen et al 2002;Singh & Chauhan 2003;Yu et al 2005Yu et al , 2009Yan et al 2006;Liu et al 2007;Jing et al 2008;Yu & He 2014). To our knowledge, only methyl arsenates, tribenuron-methyl, and amidosulphuron have been used to produce commercial hybrid seeds on a large scale (Guan & Stringam 1998;Yu et al 2006Yu et al , 2009Yu & He 2014).…”

“…Tribenuron-methyl (Yu et al 2006; Zhang et al *Yu-Feng Cheng, Jian-Min Cui, and Zhan-Jie Li equally contributed to this work and were co-first authors. doi: 10.17221/233/2014doi: 10.17221/233/ -CJGPB 2010 and amidosulphuron (Yu et al 2009), developed by DuPont (Wilmington,USA) and Aventis (Paris, France), respectively, do not meet the requirement of breeding practice in China. Currently, several dozen commercial hybrids based on CHA-induced male sterility have been registered according to the Bulletin of the Chinese National Crop Variety Approval Committee (Guan 2012).…”