Efficient delivery of anticancer drug MTX through MTX-LDH nanohybrid system

Oh, Jae‐Min; Park, Man; Kim, Sang-Tae; Jung, Jin-Young; Kang, Yong-Gu; Choy, Jin‐Ho

doi:10.1016/j.jpcs.2006.01.033

Cited by 161 publications

(89 citation statements)

References 8 publications

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“…The (00l) reflections of PMAE appeared at a low 2θ angle, ie, 3.86°, 8 Figure 1B and C shows the intercalation of perindopril erbumine into Zn/Al-layered double hydroxide accomplished by the ion-exchange method (PZAE) and direct method (PZAC), respectively. 21 It can be seen that the d-spacing (d 003 ) of the PMAE and PZAE nanocomposites is 22.85 Å and 21.70 Å, respectively.…”

Section: Resultsmentioning

confidence: 99%

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Comparative study of Mg/Al- and Zn/Al-layered double hydroxide-perindopril erbumine nanocomposites for inhibition of angiotensin-converting enzyme

Ali

Al‐Qubaisi²,

Hussein³

et al. 2012

IJN

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Abstract:The intercalation of a drug active, perindopril, into Mg/Al-layered double hydroxide for the formation of a new nanocomposite, PMAE, was accomplished using a simple ion exchange technique. A relatively high loading percentage of perindopril of about 36.5% (w/w) indicates that intercalation of the active took place in the Mg/Al inorganic interlayer. Intercalation was further supported by Fourier transform infrared spectroscopy, and thermal analysis shows markedly enhanced thermal stability of the active. The release of perindopril from the nanocomposite occurred in a controlled manner governed by pseudo-second order kinetics. MTT assay showed no cytotoxicity effects from either Mg/Al-layered double hydroxide or its nanocomposite, PMAE. Mg/Al-layered double hydroxide showed angiotensin-converting enzyme inhibitory activity, with 5.6% inhibition after 90 minutes of incubation. On incubation of angiotensin-converting enzyme with 0.5 µg/mL of the PMAE nanocomposite, inhibition of the enzyme increased from 56.6% to 70.6% at 30 and 90 minutes, respectively. These results are comparable with data reported in the literature for Zn/Al-perindopril.

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Section: Resultsmentioning

confidence: 99%

“…7 Because of these advantages, many pharmaceutically active compounds have been intercalated in order to prolong their action. These include methotrexate, 8,9 captopril, 10 the anti-inflammatory drugs, fenbufen, diclofenac, ibuprofen, and camptothecin, 5,[11][12][13] as well as the statin agents, pravastatin and fluvastatin.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of Mg/Al- and Zn/Al-layered double hydroxide-perindopril erbumine nanocomposites for inhibition of angiotensin-converting enzyme

Ali

Al‐Qubaisi²,

Hussein³

et al. 2012

IJN

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“…A MTX-Mg/Al nanohybrid had a much stronger inhibition effect on the proliferation of human MNNG-HOS osteosarcoma cancer cells compared with unbound MTX. 1 Various methods have been used to intercalate drugs as guest molecules into LDHs, but the most common methods are direct coprecipitation and ion exchange. 21 Compared with the ion-exchange method, the direct coprecipitation method produces a large quantity of the nanocomposite and there is more risk with this method of carbon dioxide uptake and the incorporation of unwanted hydroxide anions in the reaction mixture.…”

Section: Introductionmentioning

confidence: 99%

Preparation and controlled-release studies of a protocatechuic acid-magnesium/aluminum-layered double hydroxide nanocomposite

Barahuie¹,

Hussein²,

Hussein‐Al‐Ali³

et al. 2013

IJN

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Abstract:In the study reported here, magnesium/aluminum (Mg/Al)-layered double hydroxide (LDH) was intercalated with an anticancer drug, protocatechuic acid, using ionexchange and direct coprecipitation methods, with the resultant products labeled according to the method used to produce them: "PANE" (ie, protocatechuic acid-Mg/Al nanocomposite synthesized using the ion-exchange method) and "PAND" (ie, protocatechuic acid-Mg/Al nanocomposite synthesized using the direct method), respectively. Powder X-ray diffraction and Fourier transform infrared spectroscopy confirmed the intercalation of protocatechuic acid into the inter-galleries of Mg/Al-LDH. The protocatechuic acid between the interlayers of PANE and PAND was found to be a monolayer, with an angle from the z-axis of 8° for PANE and 15° for PAND. Thermogravimetric and differential thermogravimetric analysis results revealed that the thermal stability of protocatechuic acid was markedly enhanced upon intercalation. The loading of protocatechuic acid in PANE and PAND was estimated to be about 24.5% and 27.5% (w/w), respectively. The in vitro release study of protocatechuic acid from PANE and PAND in phosphate-buffered saline at pH 7.4, 5.3, and 4.8 revealed that the nanocomposites had a sustained release property. After 72 hours incubation of PANE and PAND with MCF-7 human breast cancer and HeLa human cervical cancer cell lines, it was found that the nanocomposites had suppressed the growth of these cancer cells, with a half maximal inhibitory concentration of 35.6 μg/mL for PANE and 36.0 μg/mL for PAND for MCF-7 cells, and 19.8 μg/mL for PANE and 30.3 μg/mL for PAND for HeLa cells. No half maximal inhibitory concentration for either nanocomposite was found for 3T3 cells.

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“…The IC 50 value (at which concentration, 50 % of inhibition is measured) of MTX-LDH in MNNG/HOS cells was about 2.5 lower than that of MTX only, indicating that MTX-LDH is more effectively suppressing the proliferation of cancer cells than MTX only [74,75].…”

Section: Bio-ldh Nanohybrids With Targeting Functionsmentioning

confidence: 90%