Efficient plant regeneration from embryogenic suspension cultures of sweetpotato

In vitro selection of sweetpotato (Ipomoea batatas (L.) Lam.) plants tolerant to NaCl was achieved using embryogenic suspension cultures of sweetpotato cv. Lizixiang and gamma-ray induced mutation. Cell aggregates from embryogenic suspension cultures of Lizixiang were irradiated with 80 Gy gamma-ray, and 1 week after irradiation they were cultured in a selective medium containing 342 mM NaCl for in vitro selection. A total of 276 plants were regenerated from the irradiated 2,783 cell aggregates by a two-step in vitro selection procedure. After the regenerated plants were propagated into plant lines on the basal medium, they were cultured on the medium supplemented with 86, 171, 257 and 342 mM NaCl, respectively, in order to evaluate their in vitro salt tolerance. Of them 18 plant lines showed significantly higher in vitro salt tolerance than control plants. Proline and superoxide dismutase (SOD) were more accumulated in these 18 plant lines than in control plants when both were exposed to NaCl. Salt tolerance of the 18 plant lines was further evaluated with Hoalgland solution containing different concentrations of NaCl in a greenhouse. The results indicated that 3 of them had significantly better growth and rooting ability than the remaining 15 plant lines and control plants at 171 mM NaCl.

Section: Methodsmentioning

confidence: 99%

“…We succeeded in developing an efficient system of plant regeneration from embryogenic suspension cultures for a wide range of sweetpotato cultivars (Liu et al 2001). This embryogenic suspension culture system has been successfully used in somatic hybridization and genetic transformation of sweetpotato (Guo et al 2006;Yu et al 2007).…”

Section: Introductionmentioning

confidence: 99%

In vitro selection and identification of sweetpotato (Ipomoea batatas (L.) Lam.) plants tolerant to NaCl

Han

Wang

et al. 2008

“…Xushu 18 (2n = 6x = 90), a commercial cultivar in China, and its wild relative I. cairica L. (2n = 2x = 30) were employed in this study. Embryogenic suspension cultures of Xushu 18 and young leaves of in vitro-grown plants of I. cairica L. were prepared as reported by Liu et al (2001) and Liu et al (1990), respectively, for the isolation of protoplasts.…”

Section: Plant Materialsmentioning

confidence: 99%

Regeneration of plants from Ipomoea cairica L. protoplasts and production of somatic hybrids between I. cairica L. and sweetpotato, I. batatas (L.) Lam.

Guo

Liu

Zhai

et al. 2006

Self Cite

Plants were regenerated from mesophyll protoplasts of Ipomoea cairica L., a wild relative of sweetpotato (Ipomoea batatas (L.) Lam.), and somatic hybrids between I. cairica L. and sweetpotato cv. Xushu 18 were obtained by PEG-mediated method. I. cairica L. protoplasts were isolated from the leaves of in vitro grown plants and cultured in a modified MS medium containing 0.05 mg l -1 2,4-D and 0.5 mg l -1 kinetin. Nine weeks after plating, the obtained small calluses up to about 2 mm in diameter were transferred to solid MS medium supplemented with 0.05 mg l -1 2,4-D and 0.5 mg l -1 kinetin for callus proliferation. Three weeks after transfer, the calluses were transferred to MS medium supplemented with 0-1.0 mg l -1 IAA and 1.0-3.0 mg l -1 BAP and further to hormone-free MS medium for plant regeneration. The frequencies of calluses forming plants ranged from 6.0% to 41.3% based on the different concentrations of IAA and BAP, and 2.0 mg l -1 BAP gave the highest regeneration frequency of protoplast-derived calluses in I. cairica L.. The regenerated plants, when transferred to soil, showed 100% survival. No morphological variations were observed. Mesophyll protoplasts of I. cairica L. were fused with protoplasts isolated from embryogenic suspension cultures of Xushu 18 by PEG-mediated method. The fused products were cultured with the best protoplast culture system of I. cairica L.. Finally, 114 plants were produced from 63 of the 182 calluses derived from the fused protoplasts, and 46 plants of them were confirmed to be somatic hybrids through peroxidase isozyme, RAPD, morphological and cytological analyses.

“…Sweetpotato has long been considered a recalcitrant species for plant regeneration (Sihachakr et al 1997). Nevertheless, a variety of methods for in vitro regeneration of diverse sweetpotato genotypes have been published within the last 30 years Liu and Cantliffe 1984;Liu et al 2001;Zheng et al 1996). Different tissues have been used as explants, which undergo either direct shoot organogenesis or embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Rapid shoot regeneration in industrial ‘high starch’ sweetpotato (Ipomoea batatas L.) genotypes

Santa-María

Pecota

Yencho

et al. 2009