Efficient production of gamma‐aminobutyric acid by engineered <i>Saccharomyces cerevisiae</i> with glutamate decarboxylases from <i>Streptomyces</i>

Yuan, Haina; Zhang, Wei; Xiao, Gongnian; Zhan, Jixun

doi:10.1002/bab.1840

Cited by 14 publications

(7 citation statements)

References 46 publications

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“…When engineered E. coli was used as the biocatalyst, the production or productivity was the highest among all the whole-cell bioconversion catalysts thus far. − For example, by considerable modification of a recombinant GABA-producing E. coli strain, 308.3 g/L GABA was produced within a single whole-cell conversion process with 44.04 g/L/h productivity and 99.6 mol % conversion in a 5 L bioreactor .…”

Section: Introductionmentioning

confidence: 99%

Integrating Enzyme Evolution and Metabolic Engineering to Improve the Productivity of Γ-Aminobutyric Acid by Whole-Cell Biosynthesis in Escherichia Coli

Yang

Huo

Yiping

et al. 2023

J. Agric. Food Chem.

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γ-Aminobutyric acid (GABA) is used widely in various fields, such as agriculture, food, pharmaceuticals, and biobased chemicals. Based on glutamate decarboxylase (GadBM4) derived from our previous work, three mutants, GadM4-2, GadM4-8, and GadM4-31, were obtained by integrating enzyme evolution and high-throughput screening methods. The GABA productivity obtained through whole-cell bioconversion using recombinant Escherichia coli cells harboring mutant GadBM4-2 was enhanced by 20.27% compared to that of the original GadBM4. Further introduction of the central regulator GadE of the acid resistance system and the enzymes from the deoxyxylulose-5-phosphate-independent pyridoxal 5′-phosphate biosynthesis pathway resulted in a 24.92% improvement in GABA productivity, reaching 76.70 g/L/h without any cofactor addition with a greater than 99% conversion ratio. Finally, when one-step bioconversion was applied for the whole-cell catalysis in a 5 L bioreactor, the titer of GABA reached 307.5 ± 5.94 g/L with a productivity of 61.49 g/L/h by using crude l-glutamic acid (l-Glu) as the substrate. Thus, the biocatalyst constructed above combined with the whole-cell bioconversion method represents an effective approach for industrial GABA production.

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Section: Introductionmentioning

confidence: 99%

Integrating Enzyme Evolution and Metabolic Engineering to Improve the Productivity of Γ-Aminobutyric Acid by Whole-Cell Biosynthesis in Escherichia Coli

Yang

Huo

Yiping

et al. 2023

J. Agric. Food Chem.

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“…Similarly, Yuan et al. reported the production of 62.6 g L −1 (60.8 %) GABA from 0.1 M glutamate by engineering Saccharomyces cerevisiae expressing GAD from Streptomyces 99. Yuan also reported similar work on an engineered E .coli strain which expressed GAD from Streptomyces and produced and 2771 g L −1 (67 %) GABA 100.…”

Section: Deamination Of Amino Acids For Pharmaceutical and Food Industry Applicationsmentioning

confidence: 82%

Nitrogen‐Neutral Amino Acids Refinery: Deamination of Amino Acids for Bio‐Alcohol and Ammonia Production

Choi

2021

ChemBioEng Reviews

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Amino acids are a valuable bioresource that contains both the acid form of carbon and the amine group of nitrogen. Amino acids not only constitute proteins and the building blocks for living organisms but also provide a key intermediate for controlling the nitrogen balance in cells. The production of amino acids using microorganisms has, along with the development of biotechnology, created a large market for biochemicals. In parallel, there have been various biochemical production methods using pulmonary proteins or direct amino acids as biomass through enzymatic bioconversion or whole‐cell biotransformation. The deamination of amino acids facilitated the applications of 2‐keto and acrylic acids, in particular, for biofuels of bio alcohol, pharmaceuticals, cosmetics, as building blocks for polymers, and other versatile industrial chemicals. In this review, we report on the application of amino acids derived from biochemicals initiated by deamination reactions using deaminase, ammonia‐lyase, and oxidase enzymes for biotechnology applications.

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“…Engineered S. cerevisiae was successfully used to produce a high level of natural products from other sources, for example, artemisinic acid up to 100 mg/L [71], tetrahydrocannabinolic acid up to 8.0 mg/L, cannabidiolic acid, and tetrahydrocannabivarinic acid up to 4.8 mg/L [72]. Engineered S. cerevisiae also produced some natural products from Streptomyces with high titers, such as gamma aminobutyric acid up to 62.6 g/L [73] and cannabigerolic acid up to 299.8 mg/L [74].…”

Section: Other Hostsmentioning

confidence: 99%

Recent Advances in the Heterologous Biosynthesis of Natural Products from Streptomyces

et al. 2021

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Streptomyces is a significant source of natural products that are used as therapeutic antibiotics, anticancer and antitumor agents, pesticides, and dyes. Recently, with the advances in metabolite analysis, many new secondary metabolites have been characterized. Moreover, genome mining approaches demonstrate that many silent and cryptic biosynthetic gene clusters (BGCs) and many secondary metabolites are produced in very low amounts under laboratory conditions. One strain many compounds (OSMAC), overexpression/deletion of regulatory genes, ribosome engineering, and promoter replacement have been utilized to activate or enhance the production titer of target compounds. Hence, the heterologous expression of BGCs by transferring to a suitable production platform has been successfully employed for the detection, characterization, and yield quantity production of many secondary metabolites. In this review, we introduce the systematic approach for the heterologous production of secondary metabolites from Streptomyces in Streptomyces and other hosts, the genome analysis tools, the host selection, and the development of genetic control elements for heterologous expression and the production of secondary metabolites.

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Efficient production of gamma‐aminobutyric acid by engineered Saccharomyces cerevisiae with glutamate decarboxylases from Streptomyces

Cited by 14 publications

References 46 publications

Integrating Enzyme Evolution and Metabolic Engineering to Improve the Productivity of Γ-Aminobutyric Acid by Whole-Cell Biosynthesis in Escherichia Coli

Integrating Enzyme Evolution and Metabolic Engineering to Improve the Productivity of Γ-Aminobutyric Acid by Whole-Cell Biosynthesis in Escherichia Coli

Nitrogen‐Neutral Amino Acids Refinery: Deamination of Amino Acids for Bio‐Alcohol and Ammonia Production

Recent Advances in the Heterologous Biosynthesis of Natural Products from Streptomyces

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