Efficient production of l-lactic acid from xylose by a recombinant Candida utilis strain

Tamakawa, Hideyuki; Ikushima, Shigehito; Yoshida, Satoshi

doi:10.1016/j.jbiosc.2011.09.002

Cited by 44 publications

(26 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4). Besides P. stipitis, other microbes including Candida sonorensis, R. oryzae, and Candida utilis have been engineered to produce lactic acid from xylose, but until now, lactic acid production from xylose-fermenting S. cerevisiae has not been reported (Maas et al 2006;Tamakawa et al 2012). In our study, the nearly undetectable production of ethanol was achieved without deletion or disruption of pdc or adh genes, which has been previously studied for producing lactic acid from glucose by S. cerevisiae Saitoh et al 2005).…”

Section: Discussionmentioning

confidence: 81%

Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion

Turner

Zhang

Kim

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Production of lactic acid from renewable sugars has received growing attention as lactic acid can be used for making renewable and bio-based plastics. However, most prior studies have focused on production of lactic acid from glucose despite that cellulosic hydrolysates contain xylose as well as glucose. Microbial strains capable of fermenting both glucose and xylose into lactic acid are needed for sustainable and economic lactic acid production. In this study, we introduced a lactic acid-producing pathway into an engineered Saccharomyces cerevisiae capable of fermenting xylose. Specifically, ldhA from the fungi Rhizopus oryzae was overexpressed under the control of the PGK1 promoter through integration of the expression cassette in the chromosome. The resulting strain exhibited a high lactate dehydrogenase activity and produced lactic acid from glucose or xylose. Interestingly, we observed that the engineered strain exhibited substrate-dependent product formation. When the engineered yeast was cultured on glucose, the major fermentation product was ethanol while lactic acid was a minor product. In contrast, the engineered yeast produced lactic acid almost exclusively when cultured on xylose under oxygen-limited conditions. The yields of ethanol and lactic acid from glucose were 0.31 g ethanol/g glucose and 0.22 g lactic acid/g glucose, respectively. On xylose, the yields of ethanol and lactic acid were <0.01 g ethanol/g xylose and 0.69 g lactic acid/g xylose, respectively. These results demonstrate that lactic acid can be produced from xylose with a high yield by S. cerevisiae without deleting pyruvate decarboxylase, and the formation patterns of fermentations can be altered by substrates.

show abstract

Section: Discussionmentioning

confidence: 81%

Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion

Turner

Zhang

Kim

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…High levels of lactate (95.1 % conversion from glucose) were achieved by expressing Bos taurus lactate dehydrogenase in a C. utilis pdc1 mutant lacking pyruvate decarboxylase (Ikushima et al 2009b). Subsequently, glucose was replaced by xylose as an inexpensive carbon source for lactate production using a strain synthesizing a heterologous fungal NADH-referring mutated xylose reductase (XR), as well as heterologous fungal xylitol dehydrogenase and xylulokinase in the pdc1 mutant background (Tamakawa et al 2010a). In the absence of the pdc1 mutation, these heterologous enzymes led to 0.4 g ethanol per gram of xylose per liter by C. utilis (Tamakawa et al 2010b(Tamakawa et al , 2013a(Tamakawa et al , 2013b.…”

Section: Utilis Recombinant Products Intracellular Productsmentioning

confidence: 99%

Candida utilis and Cyberlindnera (Pichia) jadinii: yeast relatives with expanding applications

Buerth

Tielker

Ernst

2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The yeast Candida utilis is used as a food additive and as a host for heterologous gene expression to produce various metabolites and proteins. Reliable protocols for intracellular production of recombinant proteins are available for C. utilis and have now been expanded to secrete proteins into the growth medium or to achieve surface display by linkage to a cell wall protein. A recombinant C. utilis strain was recently shown to induce oral tolerance in a mouse model of multiple sclerosis suggesting future applications in autoimmune therapy. Whole genome sequencing of C. utilis and its presumed parent Cyberlindnera (Pichia) jadinii demonstrated different ploidy but high sequence identity, consistent with identical recombinant technologies for both yeasts. C. jadinii was recently described as an antagonist to the important human fungal pathogen Candida albicans suggesting its use as a probiotic agent. The review summarizes the status of recombinant protein production in C. utilis, as well as current and future biotechnological and medical applications of C. utilis and C. jadinii.

show abstract

“…Candida utilis expressing xylitol dehydrogenase, xylulokinase and a xylose reductase mutated to favor NAD over NADP ? generated 67 g L-lactate l -1 from xylose in 72 h (Tamakawa et al 2012). The yeast Pichia stipitis is advantageous because it naturally ferments xylose, primarily to ethanol (Prior et al 1989).…”

Section: Decrease Of By-products From Pentosesmentioning

confidence: 99%

Microbial production of lactic acid

Eiteman

Ramalingam

2015

Biotechnol Lett

View full text Add to dashboard Cite

Lactic acid is an important commodity chemical having a wide range of applications. Microbial production effectively competes with chemical synthesis methods because biochemical synthesis permits the generation of either one of the two enantiomers with high optical purity at high yield and titer, a result which is particularly beneficial for the production of poly(lactic acid) polymers having specific properties. The commercial viability of microbial lactic acid production relies on utilization of inexpensive carbon substrates derived from agricultural or waste resources. Therefore, optimal lactic acid formation requires an understanding and engineering of both the competing pathways involved in carbohydrate metabolism, as well as pathways leading to potential by-products which both affect product yield. Recent research leverages those biochemical pathways, while researchers also continue to seek strains with improved tolerance and ability to perform under desirable industrial conditions, for example, of pH and temperature.

show abstract

Efficient production of l-lactic acid from xylose by a recombinant Candida utilis strain

Cited by 44 publications

References 19 publications

Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion

Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion

Candida utilis and Cyberlindnera (Pichia) jadinii: yeast relatives with expanding applications

Microbial production of lactic acid

Contact Info

Product

Resources

About