Efflux Transporters: Newly Appreciated Roles in Protection against Pollutants

Epel, David; Luckenbach, Till; Stevenson, Charlotte N.; MacManus-Spencer, Laura A.; Hamdoun, Amro; Smital, Tvrtko

doi:10.1021/es087187v

Cited by 158 publications

(122 citation statements)

References 39 publications

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“…This bioassay revealed the presence of MXR inhibitors in all examined samples, particularly in the diluted samples. We have thus confirmed the concept that the measurement of the MXR inhibition can be utilized as a valuable specific biomarker of environmental contamination (Epel et al, 2008;Hackenberger et al, 2012), and suggest the use of E. fetida exposure as a tool for constant bio-monitoring.…”

Section: Earthworms As Bioindicators Mxr Inhibitionsupporting

confidence: 71%

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Popović

Strunjak‐Perović

Klobučar

et al. 2015

Science of The Total Environment

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Relating the treated wastewater quality and its impact on organismic biosensors (Prussian carp, Carassius gibelio and earthworm, Eisenia fetida) was the main objective of the study. The impact on health status of fish living downstream, microbiological contamination and antimicrobial resistance, fish tissue structure, blood biochemistry, oxidative stress, genotoxic effects, as well as multixenobiotic resistance mechanism (MXR) was assessed. Treated wastewater discharged from the WWTP modified the environmental parameters and xenobiotic concentrations of the receiving surface waters. Potential bacterial pathogens from fish and respective waters were found in relatively low numbers, although they comprised aeromonads with a zoonotic potential. High resistance profiles were determined towards the tested antimicrobial compounds, mostly sulfamethoxazole and erytrhomycin. Histopathology primarily revealed gill lamellar fusion and reduction of interlamellar spaces of effluent fish. A significant increase in plasma values of urea, total proteins, albumins and triglycerides and a significant decrease in the activity of plasma superoxide dismutase were noted in carp from the effluent-receiving canal. Micronucleus test did not reveal significant differences between the examined groups, but a higher frequency of erythrocyte nuclear abnormalities was found in fish sampled from the effluent-receiving canal. Earthworms indicated to the presence of MXR inhibitors in water and sludge samples, thus proving as a sensitive sentinel organism for environmental pollutants. The integrative approach of this study could serve as a guiding principle in conducting evaluations of the aquatic habitat health in complex bio-monitoring studies.Response to Reviewers: The response is attached as a separate file. We request that this manuscript be considered for publication in Science of the Total Environment as an original, full paper. This manuscript has not been published and is not under consideration for publication in any other journal. All authors concur with the submission.The manuscript is a result of a comprehensive study which integrated various disciplines and research fields. The authors decided to present it as a whole rather than splitting it in two or more papers, for a better understanding of the discussed subject. We believe we have presented a potential guiding principle for conducting evaluations of the aquatic habitat health in complex bio-monitoring studies.I will be serving as the corresponding author for the manuscript. We hope that it is found to be interesting and appropriate for publication in Science of the Total Environment. Should you have any questions, please do not hesitate to contact me.Yours truly, Natalija Topic Popovic, DVM, PhD Response: Thank you for this clarification. We have taken a whole different approach and completely rewritten all sections relating to the PCA as they were indeed confusing, and created a new biplot for plasma parameters. We hope that the new text, as inserted in the respect...

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Section: Earthworms As Bioindicators Mxr Inhibitionsupporting

confidence: 71%

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Popović

Strunjak‐Perović

Klobučar

et al. 2015

Science of The Total Environment

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“…Moderate expression of P-gp activity, thus moderate induction of MXR transporter activity in bivalves to environmental contaminants, has already been reported both for laboratory tests and in situ surveys (Eufemia and Epel 2000;Smital et al 2003). Epel et al (2008), in their review article, commented that this phenomenon is an indication of several alternatives: (1) increased P-gp (MXR) levels are not an important protective response; (2) the level of transporter activity is already set to the expected historical load of xenobiotics; and (3) a small increase in transporter activity is adequate to protect the organism. Our results seem to support the latter hypothesis, but to prove this in additional studies with more complex polluted sediments must be performed.…”

mentioning

confidence: 85%

Evaluation of Multixenobiotic Resistance in Dreissenid Mussels as a Screening Tool for Toxicity in Freshwater Sediments

Ács

Imre²,

Kiss³

et al. 2015

Arch Environ Contam Toxicol

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“…The cellular multixenobiotic resistance (MXR) system represents a broad-scale defense mechanism that protects cells and organisms against endogenous and exogenous toxicants (Epel et al, 2008). MXR is mediated by membrane transport proteins from the ATP binding cassette (ABC) protein family, which recognize a wide variety of potential xenobiotics as substrates, and pump them out of the cell in an energy-dependent, ATP-driven process (Navarro et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effects of fluoxetine on behavior, antioxidant enzyme systems, and multixenobiotic resistance in the Asian clam Corbicula fluminea

et al. 2015

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h i g h l i g h t sGR, TR, ABCB1, ABCC1, and ABCG2 genes were identified in the clam. The siphoning behavior of the clam was inhibited by FLX. activity and malondialdehyde (MDA) content markedly increased (p < 0.05). Moreover, transcription of thioredoxin reductase (TR), glutathione peroxidase (GPx) and Glutathione S-transferase pi class (GSTpi) was significantly upregulated (p < 0.05), whereas glutathione reductase (GR) was markedly downregulated (p < 0.05). These findings suggest that FLX affects behavior and induces oxidative stress in C. fluminea. The downregulation of ATP-binding cassette (ABC) transportor genes (ABCB1, ABCC1 and ABCG2) transporter genes indicated that FLX might suppress the multixenobiotic resistance (MXR) system in C. fluminea. Our results provide new insights into the adverse effects of FLX on the cellular detoxification and MXR system of C. fluminea.

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Efflux Transporters: Newly Appreciated Roles in Protection against Pollutants

Cited by 158 publications

References 39 publications

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Impact of treated wastewater on organismic biosensors at various levels of biological organization

Evaluation of Multixenobiotic Resistance in Dreissenid Mussels as a Screening Tool for Toxicity in Freshwater Sediments

Effects of fluoxetine on behavior, antioxidant enzyme systems, and multixenobiotic resistance in the Asian clam Corbicula fluminea

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