Eficiência fotossintética e crescimento de genótipos de abacaxizeiro cultivados in vitro em diferentes qualidades de luz, tipos de frasco de cultivo e concentrações de sacarose

Couto, Tarcísio Rangel do; Silva, Jefferson Rangel da; Netto, Alena Torres; Carvalho, Virgínia Silva; Campostrini, Eliemar

doi:10.1590/0100-2945-167/13

Cited by 9 publications

(7 citation statements)

References 12 publications

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“…In this sense, when evaluating the in vitro root development of sugarcane cultivar RB867515, a 50% sucrose reduction compared to the usual concentration in culture medium (30 g L -1 ) negatively influenced root development after 60 days of cultivation, where the average values of root length and root fresh and dry weight were lower than in treatments with higher sucrose concentration (Ferreira et al, 2016). In in vitro development, photosynthesis is very low and cultures are dependent on the external support of carbohydrates (Couto et al, 2014). However, in the present study, sucrose was required for the development of Saccharum officinarum shoots, although in a concentration lower than that commonly used in in vitro cultivation.…”

Section: Resultsmentioning

confidence: 55%

In vitro multiplication of sugarcane with different nitrogen and sucrose concentrations

Alcantara¹,

Machado²,

Oliveira³

et al. 2019

Científica

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Section: Resultsmentioning

confidence: 55%

In vitro multiplication of sugarcane with different nitrogen and sucrose concentrations

Alcantara¹,

Machado²,

Oliveira³

et al. 2019

Científica

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“…Algumas explicações podem ser generalizadas, no entanto, essas variações são em sua grande maioria distintas não somente para a espécie, mas também entre cultivares. Esse padrão foi verificado em abacaxi onde a luz vermelha foi mais eficiente que a luz branca no incremento de matéria seca de plântulas de abacaxi pertencente às cultivares Vitória e IAC fantástico (COUTO et al, 2014). In vitro grande parte dos trabalhos demonstraram que a luz vermelha + azul tem um papel no ganho de massa tanto da raiz como da parte área (GU et al,2012;MALUTA et al, 2013).…”

Section: Resultsunclassified

ESPECTROS LUMINOSOS NO DESENVOLVIMENTO DE PLÂNTULAS DE Curcuma longa CULTIVADAS IN VITRO

Ferrari

Antoniazzi

Nascimento

et al. 2017

ArqVet

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Umuarama, v. 19, n. 4, p. 247-251, out./dez. 2016. RESUMO:Curcuma longa é uma espécie asiática perene e rizomatosa com grandes propriedades medicinais, alimentícias e ornamentais. No processo micropropagativo carece de muitas informações dentre a faixa espectral mais adequada ao seu desenvolvimento. Sendo assim, o objetivo desse trabalho foi avaliar diferentes filmes espectrais no desenvolvimento de plântulas de C. longa cultivadas in vitro. Para tanto, brotações de C. longa foram inoculadas em meio Murashig e Skoog e suplementado com 30 g/L de sacarose, 6,5 g/L de ágar 4, 44 μM/L de Benzil aminopurina (BAP) e 1,08 μM/L ácido naftaleno acético (ANA). O pH do meio foi ajustado para 5,8. Os brotos foram submetidos a diferentes intensidades e qualidade espectral de luz: branco, vermelho, amarelo, azul e verde. As plântulas foram mantidas em sala de crescimento em luz constante de 25C° e fotoperíodo de 24 horas por 145 dias. Foram avaliadas características morfológicas e anatômicas. Os resultados obtidos demonstraram que as diferenças espectraris propiciaram diferenças no desenvolvimento das plântulas de C. longa e também na contaminação in vitro. O diâmetro da base das plântulas, a massa seca e fresca da parte área e raiz foram influenciadas pelas diferentes faixas espectrais, sendo que de modo geral os filmes, amarelo e branco foram os que propiciaram maiores valores para essas características. Já o filme verde foi o que menos favoreceu o ganho de massa das plântulas, além disso, alta taxa de contaminação foi observada na presença desse filme. PALAVRAS-CHAVE: Açafrão-da-terra. Filmes coloridos. Micropropagação. Morfologia. LUMINOUS SPECTRUM IN THE DEVELOPMENT OF IN-VITRO Curcuma longa SEEDLINGSABSTRACT: Curcuma longa is a perennial Asiatic rhizomelic species with important medicinal, food and ornamental properties. However, its micro-propagation process lacks information regarding the most adequate spectral range for its development. Thus, this work aimed to evaluate different spectrum films in the development of in vitro C. longa seedlings. In order to do this, C. longa sprouts were inoculated in Murashig and Skoog media supplemented with 30 g/L of sucrose, 6.5 g/L of agar 4, 44 μM/L of benzylaminopurine (BAP) and 1.08 μM/L naphthalene acetic acid (ANA). The pH was adjusted to 5.8. Sprouts were put under different light spectrum intensity and quality: white, red, yellow, blue and green. The seedlings were maintained in a growth room with constant light at 25 ºC and a 24-hours photoperiod for 145 days. Morphological and anatomical characteristics were analyzed. The results demonstrated that spectral differences propitiated differences in the development of C. longa seedlings, and also in the in vitro contamination. The seedling base diameter, aerial and root dry and fresh mass were influenced by the different spectral ranges, with the yellow and white ranges being those that resulting the highest values for each characteristic. The green spectrum was the least favorable for the seedling regarding mass gain, as we...

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“…This result is expected because the tubes allowed the normalization of these gases contents with the external environment (Table 1, 2 and 3). In addition, 28 of the 29 surviving treatments Figure 1) contained sucrose in the culture medium, which interferes with photosynthesis (COUTO et al, 2014). Because of these interferences in photosynthesis and gas exchange with the external environment, the CO 2 /0 2 ratio has not been thoroughly analyzed, despite its importance in photorespiration, biomass production and metabolites (VANCE;SPALDING, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…It is called photoautotrophic micropropagation when there is a total absence of sucrose in the culture medium, however when sucrose is added there is photomixotrophic micropropagation (HOANG et al, 2017). According to Couto et al (2014), the presence of sucrose in the culture medium may inhibit the photosynthetic assimilation of CO 2 . However, Fuentes et al (2005) state that the presence of exogenous sucrose may increase plant survival and growth during the acclimatization phase.…”

Section: Introductionmentioning

confidence: 99%

Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata

et al. 2020

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The biotechnological interest in genus Physalis has increased in the last decades, however, there are still few micropropagation studies of this genus. The objective of this study was to evaluate P. angulata photoautotrophic and photomixotrophic micropropagation with gas exchange under seven light spectra and five concentrations of sucrose. Lighting were yellow, blue, white, red, green, red + blue LEDs and natural light filtered by mesh. Sucrose concentrations were 0, 7.5, 15, 22.5 and 30 g.L-1. Phytotechnical, anatomical features and photopigment contents were evaluated through stem and root segment length, leaf number, leaf area, chlorophyll a and b contents, carotenoids, adaxial epidermis, palisadic and spongy parenchyma and abaxial epidermis. The data were compared by Scott-Knott's mean test and principal components analysis using the R software. Comparing the variables within lighting types, it was observed that only the screen treatment, screen-filtered natural illumination, obtained assessment in all variables. Comparing the levels of sucrose, it was observed that the treatment 15 g.L-1 sucrose obtained the highest number of averages with maximum evaluation. It was concluded that the natural light filtered by screen with 50% of shading allowed the photoautotrophic micropropagation of P. angulata. Better development results were observed in photomixotrophic micropropagation with 15 g.L-1 of sucrose.

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Eficiência fotossintética e crescimento de genótipos de abacaxizeiro cultivados in vitro em diferentes qualidades de luz, tipos de frasco de cultivo e concentrações de sacarose

Cited by 9 publications

References 12 publications

In vitro multiplication of sugarcane with different nitrogen and sucrose concentrations

In vitro multiplication of sugarcane with different nitrogen and sucrose concentrations

ESPECTROS LUMINOSOS NO DESENVOLVIMENTO DE PLÂNTULAS DE Curcuma longa CULTIVADAS IN VITRO

Effect of light and sucrose on photoautotrophic and photomixotrophic micropropagation of Physalis angulata

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