Ehrlichia canis phylogenetic analysis of the Borgo (Corsica) strain

Parzy, Daniel; Baudu, P.; Normand, T.; Audonnet, Jean Christophe; Davoust, Bernard

doi:10.1111/j.1469-0691.2008.02269.x

Cited by 3 publications

(6 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…D. immitis, D. repens. L. infantum and E. canis] was above 20% in both groups thereby demonstrating how intense the challenge was and confirming the very high risk of CVBD previously reported in the site study [5][6][7][8][9]. Therefore, the severe life-threatening condition of these infections as well as their zoonotic potential highlights the real need for a cost-effective chemoprophylaxis easy to implement in order to control transmission.…”

Section: Discussionsupporting

confidence: 81%

“…The prevalence rates in dogs were recorded at 21.3% [5], for D. immitis. The presence of canine ehrlichiosis was confirmed on the island [6,7]. The cumulative incidence of canine leishmaniosis throughout the past 20 years (from 1993 to 2012) was reported at 42.5% [8].…”

Section: Introductionmentioning

confidence: 89%

“…However, no data were available on the effectiveness of the MMP strategy against CVBDs in the field. In this field study, we tested the MMP protocol based on the concurrent treatment with a broad-spectrum insecticides (DPP) and a larvicidal products (milbemycine oxime-praziquantel) against the major CVBDs occurring in Corsica, an area well known for its endemicity [1,[6][7][8]].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Field Implementation of a Monthly Multi-Modal Prophylactic Strategy (Dinotefuran-Permethrin-Pyriproxyfen and Milbemycine Oxime-Praziquantel) Against Canine Vector-Borne Diseases in Corsica

Laidoudi¹,

Medkour²,

Tahir³

et al. 2020

Preprint

View full text Add to dashboard Cite

With a mild Mediterranean-type climate, Corsica is endemic for canine vector-borne diseases (CVBDs) such as dirofilariosis (a mosquitoes borne-diseases: MBDs), leishmaniosis and ehrlichiosis. The aim of this present study was to evaluate a monthly multi-modal prophylactic strategy (MMP) against CVBDs occurring in Corsica. The study was conducted as a comparative field trial in which eighty dogs allocated into two groups were included: (i) Group 1 consisted of 25 dogs under the MMP [per-os administration of 1.5 tablet of milbemycine oxime-praziquantel and a topical line-on application of a 3.6 mL solution of dinotefuran-permethrin-pyriproxyfen] and (ii) Group 2 under various real-life prophylactic treatment (RLP) based on the use of ectoparasiticide products [different formulations: deltamethrin, fluralaner, fipronil] and/or macrocyclic lactones based-products [milbemycin oxime/praziquantel, milbemycin oxime, moxidectin] during the period ranging from June to October 2017. All animals were followed for one year and had blood drawn at day 0, followed by follow-up at 6 and 12 months. Samples were screened for filariosis using molecular tools as well as for leishmaniosis and ehrlichiosis using indirect immunofluorescence assay (IFA). At the end of the study, no new cases of CVBDs were recorded within Group 1. In Group 2, the cumulative incidence of CVBDs was 20.0% (n= 11; p= 0.015) including dirofilarioses due to Dirofilaria immitis and/or D. repens, with 16.4% (n= 9; p=0.027). Ehrlichiosis was 5.5% (n= 3; p=0.241). No new cases of leishmaniosis were detected in Group 2. The data illustrated that, unlike the RLP treatment which failed to protect at least 20% of dogs, the MMP based on the concurrent administration of milbemycine oxime-praziquantel and dinotefuran-permethrin-pyriproxyfen is efficient to protect dogs against CVBDs in a high-risk area.

show abstract

Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Field Implementation of a Monthly Multi-Modal Prophylactic Strategy (Dinotefuran-Permethrin-Pyriproxyfen and Milbemycine Oxime-Praziquantel) Against Canine Vector-Borne Diseases in Corsica

Laidoudi¹,

Medkour²,

Tahir³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…E. canis is considered to have a limited host range among canines and to some extent among felids. Nucleic acid of E. canis has been detected from human beings in Venezuela (Unver et al, 2001) as well from ruminants (ovines) in Turkey and South Africa (Parzy et al, 2009). However pathogen has not been isolated from any human subject till date but raises concerns over 'One-Health' issues.…”

Section: Discussionmentioning

confidence: 99%

“…However pathogen has not been isolated from any human subject till date but raises concerns over 'One-Health' issues. Wider host range and the similarity among the geographically distinct isolates indicate a global spread of the pathogen and its vectors (Parzy et al, 2009). These hint at zoonotic potential of E. canis and its potential ability to cross species barrier of both hosts and vectors, though human infections are considered to be rare and accidental (McQuiston et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach

Mittal

Kundu

Chakravarti

et al. 2017

Preventive Veterinary Medicine

View full text Add to dashboard Cite

Canine Monocytic Ehrlichiosis (CME) is a serious tick-borne rickettsial disease affecting canine populations globally. Besides few reports from stray and pet dogs from localised geographical regions (cities/towns/small states), a comprehensive study on prevalence of Ehrlichia canis (E. canis) among working dogs from different geo-climatic zones of India was pertinently lacking. Study of CME among these dog populations was thus carried out, encompassing clinical aspects and different diagnostic methodologies viz., microscopy, serology and molecular biology. During the two-year study period, clinical specimens from 225 cases suspected of canine ehrlichiosis were examined for clinical pathology and presence of the haemoparasites. Overall prevalence of ehrlichiosis by microscopic examination, commercial dot-ELISA kit and nested PCR assay was estimated to be 1.3%, 19.1% and 5.8%, respectively, which were found to be statistically significant by McNemar Chi squared test (p<0.05). It was also observed that possibly due to widespread use of doxycycline therapy in field, CME presently does not remain a potential threat which it uses to pose earlier. However, concurrent infections of E. canis and Babesia gibsoni were found to be mostly fatal. Keeping in view of high number of apparently healthy dogs (24) out of total positive cases (46) observed during the study, it is recommended that prevalence studies on CME should also involve screening of apparently healthy dogs. Phylogenetic analysis carried on partial sequencing of 16S rRNA of E. canis strains revealed that all of the Indian strains clustered in a single clade with other E. canis species from India and rest of the world. Molecular divergence was observed among the sequences of Brazilian and American isolates which were also included in the present study. These findings have thus opened a new paradigm for planning of pragmatic control strategies against CME.

show abstract

Molecular Detection and Characterization of Ehrlichia canis Isolates from Three Geographic Regions in Mexico: A Retrospective Study

Lira-Amaya,

Beristain-Ruiz,

Racanco-Delgado

et al. 2023

Life

View full text Add to dashboard Cite

Canine monocytic ehrlichiosis (CME) is the most common tick-borne disease affecting domestic dogs and other wild canids. It has a worldwide distribution and is associated with the presence of the brown dog tick. Few studies have been conducted in Mexico to identify and characterize Ehrlichia canis genetic variability. In the present study, 111 dogs of different sex, breed, and age from three geographic regions in Mexico were included. All of them had a previous history of tick infestation and/or the presence of one or more clinical signs compatible with CME. All dogs were tested by a commercial ELISA and nested PCR assay for the detection of E. canis. In addition, we analyzed the E. canis genetic diversity from the 16S rRNA gene sequences obtained in this study, along with 15 additional sequences described for E. canis in Mexico and obtained from GeneBank. Serological detection by commercial ELISA results showed overall infection rates of 85.58% (95/111), including 73.1% (30/41) in samples from Guerrero state; 75% (15/20) in Morelos; and 100% (50/50) in Chihuahua. On the other hand, molecular detection (nPCR assay) showed 31.5% (35/111) overall infection rate, with 41.4% (17/41) in Guerrero state; 55% (11/20) in Morelos; and 14% (7/50) in Chihuahua. We observed a high 16S rRNA gene sequence conservancy in most of the E. canis isolates in the three geographical areas from Mexico, including those analyzed in this research, suggesting a common geographic origin among isolates.

show abstract

Ehrlichia canis phylogenetic analysis of the Borgo (Corsica) strain

Cited by 3 publications

References 3 publications

Field Implementation of a Monthly Multi-Modal Prophylactic Strategy (Dinotefuran-Permethrin-Pyriproxyfen and Milbemycine Oxime-Praziquantel) Against Canine Vector-Borne Diseases in Corsica

Field Implementation of a Monthly Multi-Modal Prophylactic Strategy (Dinotefuran-Permethrin-Pyriproxyfen and Milbemycine Oxime-Praziquantel) Against Canine Vector-Borne Diseases in Corsica

Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach

Molecular Detection and Characterization of Ehrlichia canis Isolates from Three Geographic Regions in Mexico: A Retrospective Study

Contact Info

Product

Resources

About