Eimeria bovis-induced modulation of the host cell proteome at the meront I stage

Lutz, Kathleen; Schmitt, Sigrid; Linder, Monica; Hermosilla, Carlos; Zahner, Horst; Taubert, Anja

doi:10.1016/j.molbiopara.2010.08.003

Cited by 13 publications

(13 citation statements)

References 65 publications

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“…alpha-actin and glucosidase, were altered in all fumagillin treated groups. Alterations in actin proteins were shown to be necessary for successful infection by intracellular apicomplexan parasites [42], [43]. Alpha glucosidase II isoforms are necessary in carbohydrate metabolism [44], and other altered proteins (Table 2) are located in mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Nosema ceranae Escapes Fumagillin Control in Honey Bees

et al. 2013

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Fumagillin is the only antibiotic approved for control of nosema disease in honey bees and has been extensively used in United States apiculture for more than 50 years for control of Nosema apis. It is toxic to mammals and must be applied seasonally and with caution to avoid residues in honey. Fumagillin degrades or is diluted in hives over the foraging season, exposing bees and the microsporidia to declining concentrations of the drug. We showed that spore production by Nosema ceranae, an emerging microsporidian pathogen in honey bees, increased in response to declining fumagillin concentrations, up to 100% higher than that of infected bees that have not been exposed to fumagillin. N. apis spore production was also higher, although not significantly so. Fumagillin inhibits the enzyme methionine aminopeptidase2 (MetAP2) in eukaryotic cells and interferes with protein modifications necessary for normal cell function. We sequenced the MetAP2 gene for apid Nosema species and determined that, although susceptibility to fumagillin differs among species, there are no apparent differences in fumagillin binding sites. Protein assays of uninfected bees showed that fumagillin altered structural and metabolic proteins in honey bee midgut tissues at concentrations that do not suppress microsporidia reproduction. The microsporidia, particularly N. ceranae, are apparently released from the suppressive effects of fumagillin at concentrations that continue to impact honey bee physiology. The current application protocol for fumagillin may exacerbate N. ceranae infection rather than suppress it.

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Section: Discussionmentioning

confidence: 99%

Nosema ceranae Escapes Fumagillin Control in Honey Bees

et al. 2013

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“…The other enzyme, ATP-dependent RNA helicase was identified from the spot 158, which are implicated in all aspects of cellular RNA metabolism and promote unwinding of RNA during splicing and translation [13]. This protease has previously been reported to exist in Eimeria bovis and Schistosoma japonicum by proteomic analysis [36]. Spots 183, 547, 71, and 331 were identified as hypothetical proteins of T. gondii with unknown function not described previously at the protein level.…”

Section: Discussionmentioning

confidence: 99%

Potential Vaccine Targets against Rabbit Coccidiosis by Immunoproteomic Analysis

et al. 2017

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The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of Eimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially with E. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti-E. stiedae sera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits with E. stiedae, which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.

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“…by proteomics tools such as 1-DE and 2-DE followed by digestion and analysis (Jiang et al, 2005;Lutz, et al, 2011). It offers many advantages: (i) visual data format (i.e.…”

Section: Iv) Mass Spectometery (Ms)mentioning

confidence: 99%