Ekstrak Etanol Teh Hijau (Camellia sinensis) Meningkatkan Kadar Folicle Stimulating Hormone (FSH) dan Jumlah Folikel Antral pada Tikus Betina yang Dipapar Sipermetrin

Abstract

Cypermethrin is an insecticide synthetic pyrethroid type that can disrupt the reproductive system. Cypermethrin is also capable of producing reactive oxygene species (ROS) so it is responsible for causing oxidative stress. Green tea (Camellia sinensis) is containing polyphenols that inhibit hydroxyl radicals and prooksidan enzymes. This study aims to prove that green tea ethanolic extract can increase FSH level and antral follicle count in female rats exposed to cypermethrin. The study was true experimental on 25 wistar female rats (Rattus norvegicus) which were divided into 5 groups; KN: no treatment, KP: cypermethrin 20 mg/kgBW/day, P1: cypermethrin 20 mg/kgBW/day + green tea ethanolic extract 7 mg/kgBW/day, P2: cypermethrin 20 mg/kgBW/day + green tea ethanolic extract 14 mg/kgBW/day, P3: cypermethrin 20 mg/kgBW/day + green tea ethanolic extract 28 mg/kgBW/day orally for 28 days. FSH level was measured by ELISA method and ovarian organ were taken to check antral follicle count with hematoxylin eosin staining method. FSH and antral follicle count in female rats showed a positive and strong with correlation coefficient of 0.742, which means an increase in FSH followed by an increase antral follicle count. There was a significantly difference in the level of FSH (p=0.005) and antral follicle count (p=0.001) between the control group and the treatment group. The green tea ethanolic extract can increase the FSH level and antral follicle count in female rats exposed to cypermethrin. Keywords