Elaboration and Validation of a Spectrophotometric Technique for Estimation of the Total Flavonoids in Anthemis Tinctoria Herb

Abstract

The aim of this work is development and validation of a spectrophotometric technique for quantification of total flavonoids in terms of rutin in potentially medicinal plant Anthemis tinctoria herb. According to the literature, Anthemis tinctoria is used in folk medicine as a hepatoprotective, hemostatic and means, and its extracts in the experiment exhibit antimicrobial, cytostatic and antioxidant activities. In this work, we used sample of Anthemis tinctoria herb harvested in the summer of 2021 during the period of mass flowering in vicinity of Perm city (valley of the Vasilievka river, Adishchevo railway station). The raw materials were dried at room temperature, with sufficient ventilation, without direct exposure to sunlight, stored separately from other types of raw materials. According to the experiments the optimal conditions for determining of total flavonoids: grinding the raw material to a size passing through a sieve with a diameter of 1 mm, the optimal concentration of ethyl alcohol extractant is 50%, the analytical wavelength is 410 nm, the extraction time in a boiling water bath is 45 minutes, the ratio of extraction and complexing agent - 2% alcohol solution of aluminum chloride - 2:5. The technique was validated in accordance with the requirements of State Pharmacopeia of Russia 14th edition, the relative error of determination with a reliable probability of 95% was 4.00%. The technique can be used in further work to conduct studies of biological activity.