The aim of this work is development and validation of a spectrophotometric technique for quantification of total flavonoids in terms of rutin in potentially medicinal plant Anthemis tinctoria herb. According to the literature, Anthemis tinctoria is used in folk medicine as a hepatoprotective, hemostatic and means, and its extracts in the experiment exhibit antimicrobial, cytostatic and antioxidant activities. In this work, we used sample of Anthemis tinctoria herb harvested in the summer of 2021 during the period of mass flowering in vicinity of Perm city (valley of the Vasilievka river, Adishchevo railway station). The raw materials were dried at room temperature, with sufficient ventilation, without direct exposure to sunlight, stored separately from other types of raw materials. According to the experiments the optimal conditions for determining of total flavonoids: grinding the raw material to a size passing through a sieve with a diameter of 1 mm, the optimal concentration of ethyl alcohol extractant is 50%, the analytical wavelength is 410 nm, the extraction time in a boiling water bath is 45 minutes, the ratio of extraction and complexing agent - 2% alcohol solution of aluminum chloride - 2:5. The technique was validated in accordance with the requirements of State Pharmacopeia of Russia 14th edition, the relative error of determination with a reliable probability of 95% was 4.00%. The technique can be used in further work to conduct studies of biological activity.

Medicinal plant raw materials have always been actively used in traditional and folk medicine. This is due to the wide range of medicinal plants therapeutic effect. Due to its therapeutic effect, Mentha asiatica Boriss is widely applied in the Republic of Tajikistan. It is used in the treatment of headaches, respiratory diseases and gastrointestinal tract disorders. To achieve the necessary level of pharmacological effect, it is necessary to take into account that the component composition of biologically active substances can change at different stages of plant development. Therefore, the aim of the study was to determine the component composition of Mentha asiatica essential oil at the stage of budding and flowering. Materials and methods: Samples of the Mentha asiatica essential oil were obtained during budding and flowering to conduct the study by method 1 of the State Pharmacopoeia XIV edition. The chemical composition of Mentha asiatica essential oils was studied by gas-liquid chromatography-mass spectrometry on Agilent 7890 A gas chromatograph with Agilent 5975C mass-selective detector. As a result, components such as limonene, eucalyptol, menthone, pulegone and isopulegone were found in the essential oil of flowering Mentha asiatica. Limonene, caryophyllene, carvone, trans-dihydrocarvone, and beta-bourbonene were found in samples of Mentha asiatica essential oil at the budding stage. It was found that all identified biologically active compounds have a wide range of therapeutic effects. Therefore, detailed studies of the Mentha asiatica essential oils pharmacological activity are of importance.

Abstract. Scots pine is a widespread plant on the territory of the Russian Federation. Scots pine cones are thought of as an advanced raw material for the research of the chemical composition and pharmacological activity. The object of this paper was the research of the essential oil component composition and antioxidative activity of young Scots pine cones collected in the Perm region. The samples of Scots pine cones were collected in the Yurlinsky, Ilyinsky, Permsky and Bardymsky districts of the Perm Region for the essential oil component composition research. The essential oil component composition was determined by the gas chromatograph with a mass selective detector Agilent 7890A. The antioxidative activity of raw materials infusions was determined by their ability to bind the free radical 2,2-diphenyl-1-picrylhydrazyl. As a result of the research, 10 components related to monoterpenes and sesquiterpenes were identified in the essential oil of Scots pine cones of all regions. The monoterpene fraction predominates in the essential oil of Scots pine cones in all districts of the research. The content of sesquiterpenes slightly increases in the essential oil of the more southern districts of the region, such as Permsky and Bardymsky. The content of sesquiterpenes in the essential oil of more southern districts grows mainly due to an increase of caryophyllene and humulene. The main component of Scots pine cones essential oil is the dicyclic monoterpene α-pinene. Basing on the results of the antioxidative activity determination, it was found that the infusions prepared from young pine cones collected in the Ilyinsky, Bardymsky and Permsky districts have the maximal ability to bind free radicals. The infusion of the pine cones from the Yurlinsky district has showed the least antioxidative activity, which can be ascribed to a high content of the monoterpene fraction in the essential oil.

Abstract. Raspberry (Rubus idaeus L.) Rosaceae is a perennial semi-shrub. Raspberry fruits are used in medicine as a diaphoretic. Underground organs are also of interest for medical use, thanks to the history of use in folk medicine and scientific research data. It is important not only to study the chemical composition, pharmacological action, but also to determine the authenticity of raw materials. The aim of the work was to determine the diagnostic signs of raspberry roots. Samples of raspberry roots were harvested on the territory of the Ilyinsky district of Perm Krai in the blueberry spruce forest. The material for the study was previously fixed in a mixture of glycerin - water - ethyl alcohol for 3 days. Cross-sections were made from the prepared materials. The micro-preparations were examined in a solution of chloral hydrate, and they were also stained with phloroglucin and Lugol's solution. When studying the cross-section of the raspberry root, it was found that the root has a secondary structure. The covering tissue is the periderm. Parenchymal cells of the phelloderm contain starch grains. Conducting system of non-ductile structure. The phloem has a typical structure for angiosperms and is located in a solid ring. The secondary xylem is represented by large vessels that surround the sclerenchyme. During the study, the following anatomical diagnostic signs of raspberry roots were established: the root has a secondary non–bulky structure, stony cells and idioblast - druze cells were found in the phelloderm. The established anatomical diagnostic features can be used in the development of normative documentation for raw materials, as well as to establish the authenticity of raspberry roots.