Electrically-driven modulation of surface-grafted RGD peptides for manipulation of cell adhesion

Abstract

Reported herein is a switchable surface that relies on electrically-induced conformational changes within surface-grafted arginine–glycine–aspartate (RGD) oligopeptides as the means of modulating cell adhesion.

Potential‐Responsive Surfaces for Manipulation of Cell Adhesion, Release, and Differentiation

“…Since the discovery of RGD peptides, numerous materials have been functionalized with these sequences for different biomedical applications . RGD peptides have been immobilized on various substrates, such as hydrogels, gold, or glass and these sequences can be modified to either promote or inhibit cell adhesion.…”

Engineering dynamic biointerfaces

“…Application of a negative charge draws the RGD tether toward the substrate by way of the positively charged amine groups, exposing the underlying hydrophilic cell resistant substrate. [35] Rather than electrically manipulating RGD, termination of PEG with a charged group controls cell adhesion by changing the orientation of the cell resist. A PEG chain capped with sulfonate folds toward a positively charged surface, while PEG-trimethylammonium folds toward a negatively charged surface.…”

Potential‐Responsive Surfaces for Manipulation of Cell Adhesion, Release, and Differentiation

“…Cell adhesion can be modulated dynamically by applying negative and positive potentials to surfaces functionalized with tailored monolayers. [13] RGD peptides have been immobilized on various substrates,s uch as hydrogels, [14] gold, [15] or glass [16] and these sequences can be modified to either promote or inhibit cell adhesion.To realize dynamic control of cell adhesion onto substrates during cell cultivation, stimuli-controlled switchable RGD surfaces have been developed and rely on the cleavage of the RGD ligands from the surface in response to electrochemical potential, [17,18] light, [19,20] and enzymes. The higher accessibility of the peptide under ap ositive applied potential causes phenotypic changes in the cells that are hallmarks of osteogenesis,w hereas lower accessibility of the peptide promoted by negative potentials leads to adipogenesis.…”

“…Since the discovery of RGD peptides,n umerous materials have been functionalized with these sequences for different biomedical applications. [13] RGD peptides have been immobilized on various substrates,s uch as hydrogels, [14] gold, [15] or glass [16] and these sequences can be modified to either promote or inhibit cell adhesion.To realize dynamic control of cell adhesion onto substrates during cell cultivation, stimuli-controlled switchable RGD surfaces have been developed and rely on the cleavage of the RGD ligands from the surface in response to electrochemical potential, [17,18] light, [19,20] and enzymes. [21] However, this type of cleavage process is irreversible and therefore cannot be used dynamically.I nn ative tissues,c ells exhibit multiple transient interactions with the local ECM, especially during tissue morphogenesis,w ound healing, and cancer progression.…”

“…Since the discovery of RGD peptides,n umerous materials have been functionalized with these sequences for different biomedical applications. [13] RGD peptides have been immobilized on various substrates,s uch as hydrogels, [14] gold, [15] or glass [16] and these sequences can be modified to either promote or inhibit cell adhesion.…”