Electroacupuncture may alleviate behavioral defects via modulation of gut microbiota in a mouse model of Parkinson’s disease

Han, Qiu-Qin; Fu, Yi; Le, Jiamei; Pilot, Adam; Cheng, Si; Chen, Pei-Qing; Wu, Hailong; Wan, Guoqing; Gu, Xuefeng

doi:10.1177/0964528421990658

Cited by 21 publications

(25 citation statements)

References 41 publications

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“…Therefore, the establishment of a benign regulation mechanism of the intestinal flora of patients with SCI is of great significance for the recovery of intestinal function. EA and microbiota‐targeted techniques, such as faecal microbiota transplantation (FMT), can promote gut microbiota remodelling, which may play a role in SCI (Han et al ., 2021 ; Jing et al ., 2021 ). However, the mechanism of gut microbiota remodelling in SCI improvement is unclear.…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture modulates the intestinal microecology to improve intestinal motility in spinal cord injury rats

Cheng

Liu

Wang

et al. 2021

Microbial Biotechnology

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Spinal cord injury (SCI) is a disease involving gastrointestinal disorders. The underlying mechanisms of the potential protective effects of electroacupuncture (EA) and 5-hydroxytryptamine (5-HT) system on SCI remain unknown. We investigated whether EA improves gut microbial dysbiosis in SCI and regulates the 5-HT system. 16S rDNA gene sequencing was applied to investigate alterations in the gut microbiome of the rats. Faecal metabolites and the expression of the 5-HT system were detected. EA and faecal microbiota transplantation (FMT) treatment facilitated intestinal transmission functional recovery and restored the colon morphology of SCI rats. The composition of the intestinal microbiota, including numbers of phylum Proteobacteria, class Clostridia, order Bacteroidales, and genus Dorea, were amplified in SCI rats, and EA and FMT significantly reshaped the intestinal microbiota. SCI resulted in disturbed metabolic conditions in rats, and the EA and FMT group showed increased amounts of catechin compared with SCI rats. SCI inhibited 5-HT system expression in the colon, which was significantly reversed by EA and FMT treatment. Therefore, EA may ameliorate SCI by modulating microbiota and metabolites and regulate the 5-HT system. Our study provides new insights into the pathogenesis and therapy of SCI from the perspective of microbiota and 5-HT regulation.

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Section: Introductionmentioning

confidence: 99%

Electroacupuncture modulates the intestinal microecology to improve intestinal motility in spinal cord injury rats

Cheng

Liu

Wang

et al. 2021

Microbial Biotechnology

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“…After the administration of MPTP, mice showed abnormalities in locomotor ability and muscle tone, similar to the clinical manifestations of human PD [41]. Studies have demonstrated that PD mice prefer the open-eld and slow-climbing time [42,43]. The detection of behavioral de cits in PD mice using behavioral methods such as open-eld and pole-climbing can aid in the potential therapeutic evaluation of drugs for PD.…”

Section: Discussionmentioning

confidence: 96%

Neuroprotective effects of morroniside against Parkinson's Disease via inhibiting oxidative stress and ferroptosis

Zhang

Jiang

et al. 2022

Preprint

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Parkinson’s disease (PD) is the second most common neurodegenerative disorder after Alzheimer disease accompanied by the death of dopaminergic neurons and brain nigrostriatal mitochondrial damage in the elderly population. The features of the disease include tremor, rigidity, postural instability, and motor retardation. The pathogenesis of Parkinson’s disease is complex, and abnormal lipid metabolism resulting in ferroptosis due to the excessive accumulation of free radicals from oxidative stress in the substantia nigra of the brain was thought to be one of the factors causing the disease. Morroniside has been reported to have significant neuroprotective effects, although it has not been studied in PD. Therefore, this study focused on determining the neuroprotective effects of morroniside (25, 50, and 100 mg/kg) on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg)-induced mice models of PD and explored 1-methyl-4-phenylpyridinium MPP+-induced ferroptosis in PC12 cells. Morroniside restored the impaired motor function in the PD mice models while reducing neuronal injury. The activation of nuclear factor erythroid 2-related factor 2/antioxidant response elements (Nrf2/ARE) by morroniside promoted antioxidation, the content of reducing agent glutathione (GSH) increased, and the level of the lipid metabolite malondialdehyde (MDA) decreased. Notably, morroniside inhibited ferroptosis in substantia nigra of the brain and PC12 cells, reduced iron levels, and upregulated the expression of the iron-regulated proteins glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11), ferritin heavy chain 1 (FTH-1), and ferroportin (FPN). More importantly, morroniside repaired the mitochondrial damage, restored the mitochondrial respiratory chain, and inhibited the production of reactive oxygen species (ROS). These data indicated that morroniside could activate the Nrf2/ARE signaling pathway to increase the antioxidant capacity, thereby inhibiting abnormal lipid metabolism and protecting dopaminergic neurons from ferroptosis in PD.

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“…NOX2 has been identified as an upstream factor controlling oxidative stress and neuroinflammation. EA stimulation alleviates behavioral changes via modulating gut microbiota and suppressing neuroinflammation in a mouse model of Parkinson’s disease ( 57 ). A recent study demonstrated that EA affects the vagal–adrenal axis in mice ( 22 ).…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture attenuates surgical pain-induced delirium-like behavior in mice via remodeling gut microbiota and dendritic spine

et al. 2022

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Surgical pain is associated with delirium in patients, and acupuncture can treat pain. However, whether electroacupuncture can attenuate the surgical pain-associated delirium via the gut–brain axis remains unknown. Leveraging a mouse model of foot incision-induced surgical pain and delirium-like behavior, we found that electroacupuncture stimulation at specific acupoints (e.g., DU20+KI1) attenuated both surgical pain and delirium-like behavior in mice. Mechanistically, mice with incision-induced surgical pain and delirium-like behavior showed gut microbiota imbalance, microglia activation in the spinal cord, somatosensory cortex, and hippocampus, as well as an enhanced dendritic spine elimination in cortex revealed by two-photon imaging. The electroacupuncture regimen that alleviated surgical pain and delirium-like behavior in mice also effectively restored the gut microbiota balance, prevented the microglia activation, and reversed the dendritic spine elimination. These data demonstrated a potentially important gut–brain interactive mechanism underlying the surgical pain-induced delirium in mice. Pending further studies, these findings revealed a possible therapeutic approach in preventing and/or treating postoperative delirium by using perioperative electroacupuncture stimulation in patients.

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Electroacupuncture may alleviate behavioral defects via modulation of gut microbiota in a mouse model of Parkinson’s disease

Cited by 21 publications

References 41 publications

Electroacupuncture modulates the intestinal microecology to improve intestinal motility in spinal cord injury rats

Electroacupuncture modulates the intestinal microecology to improve intestinal motility in spinal cord injury rats

Neuroprotective effects of morroniside against Parkinson's Disease via inhibiting oxidative stress and ferroptosis

Electroacupuncture attenuates surgical pain-induced delirium-like behavior in mice via remodeling gut microbiota and dendritic spine

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