Electrocatalytic determination of NADH by means of electrodes modified with MWCNTs and nitroaromatic compounds

Sensitive Electrochemical Determination of NADH Using an Electrode Fabricated by Intercalation of Tetrabutylammonium Ions Into Graphite Electrode

“…The produced TBA + /PGE non‐enzymatic sensor was validated by determining its ability to detect NADH electrochemically in PBS with added human blood serum samples. For this purpose, the spike method was used [55, 61]. The procedure for preparing real samples has been explained in detail in the experimental part of this article and the recovery test results are presented in Table 2.…”

“…The limit of quantification (LOQ) was calculated to be 1.55 μM (S/N = 10). The method, limit of detection, and linear range of the modified electrode developed in this study were compared with those obtained with other NADH sensors in the literature; this information is presented in Table 1 [17,18,53,[55][56][57].…”

Electrochemical sensing of NADH using 4-nitrobenzenediazonium tetrafluoroborate salt functionalized multiwalled carbon nanotubes

“…Furthermore, it is involved in adenosine triphosphate (ATP) production. [2][3][4] It stimulates energy production and neurotransmitters in living cells. 5,6 On the other hand, the role of NAD + is also very vital during the oxidative process like glycolysis of biosystems.…”

“…[7][8][9] NADH can be re-oxidized using the reactions catalyzed by enzymes like NADH dehydrogenase in the electronic transport chain. 4 Besides these, NADH is crucial in aging, carcinogenesis, gene expression, mitochondrial function, calcium homeostasis, DNA repair, and cell death. 10,11 The concentration of NADH in the human system can be related to other biological disorders like depression, cancer, diabetes, Alzheimer's and Parkinson's diseases.…”

“…Among them, functionalization using aromatic compounds containing nitro group like -4-phenylbutyl-3,5-dinitrobenzoate (3,5-DNBPh) and 4-( pyren-1-yl) butyl 3,5-dinitrobenzoate (35DNBpy) have been proposed recently for the electrochemical determination of NADH. 4,32 The molecules are immobilized on the CNT by van der Waals and π-π interactions, wherein one of the nitro groups was reduced electrochemically to hydroxyl amine which was oxidized back to a nitroso derivative. This nitroso derivative acts as a redox mediator to oxidize NADH.…”