Electrochemical and Raman Studies of the Biointeraction between Escherichia coli and Mannose in Polydiacetylene Derivative Supported on the Self-Assembled Monolayers of Octadecanethiol on a Gold Electrode

Abstract

Here, we describe a new method to study the biointeraction between Escherichia coli and mannose by using supramolecular assemblies composed of polydiacetylene supported on the self-assembled monolayer of octadecanethiol on a gold electrode. These prepared bilayer materials simply are an excellent protosystem to study a range of important sensor-related issues. The experimental results from UV-vis spectroscopy, resonance Raman spectroscopy, and electrochemistry confirm that the specific interactions between E. coli and mannose can cause conformational changes of the polydiacetylene backbone rather than simple nonspecific adsorption. Moreover, the direct electrochemical detection by polydiacetylene supramolecular assemblies not only opens a new path for the use of these membranes in the area of biosensor development but also offers new possibilities for diagnostic applications and screening for binding ligands.

Biosensor signal amplification of vesicles functionalized with glycolipid for colorimetric detection of Escherichia coli

“…This unique chromatic property has made polydiacetylenes promising candidates in the development of biosensors [1][2][3][4][5][6][7][8][9][10][11]. The colorimetric biosensors are self-assemblies of the diacetylene lipids mixed with natural or synthetic biological receptor molecules.…”

“…These simplified constructs allow the application of receptor-ligand binding to biosensor design. One of the most interesting synthetic membranes is the polydiacetylene vesicle, which possesses a chromophoric conjugated polymer in its interior [1][2][3][4][5][6][7][8][9][10][11]. The polydiacetylene serves as an optical "transducer" of molecular recognition events occurring at the vesicle interface, and signaling occurs by a change of the chromophoric unit from blue to red.…”

Covalent immobilization of redox protein via click chemistry and carbodiimide reaction: Direct electron transfer and biocatalysis

“…To define the distance between the electrode surface and the immobilization site, self-assembled monolayers (SAMs) spontaneously formed from solutions of thiol derivatives on clean gold surfaces can be used [12,13]. Moreover, the further selective derivatization of the SAMs by surface chemistry enables multiple components to be incorporated as well as allowing SAMs to be subsequently used as platforms to which further molecular or nanoscale components can be attached with controlled surface density [14][15][16]. However, the straightforward surface reaction cannot meet the demand of well-defined surface characterization because the efficiency and reliability of the conventional coupling techniques on solid surface is not easy to control (for example, low yield, and poor selectivity).…”

A Simple and Sensitive Piezoelectric Immunosensor for Cholera Toxin Based on GM1‐Incorporated Liposome Agglutination

“…Based on the sensing surface constructed by biological membrane-like lipid films with incorporated GM1 analogues, a reagentless optical biosensor was developed for rapid assay of protein toxins using the resonant mirror in-strument and SPR (surface plasmon resonance). [6][7][8] Direct homogenous detection of CT was reported based on the color changes 9,10 and fluorescence quenching 11,12 or resonant energy transfer. 13,14 Liposomes, spherical vesicles composed of a phospholipid bilayer surrounding an aqueous cavity, were originally developed to study cell membranes.…”