Electrochemical Biosensors for Determination of Anticancer Medicine Etoposide in Human Blood by Glassy Carbon Modified Electrode Based on Film of Poly (L-Lysine) with MWCNTS

Abstract

Sensitive, selective and reproducible electrochemical sensors were developed for the electroanalysis of Etoposide (anticancer drug) based on L-lysine film, using the PLY-MWCNTS/GCE sensor. The PLY-MWCNTSs films on modified electrode exhibited very good conductivity. Cyclic voltammetry (CV) was applied to examine the electrochemical behavior of PLY film and electrochemical response toward ETO. The PLY-MWCNTS/GCE sensor for detection of the ETO and also the experimental parameters such as film thickness, solution pH, time and accumulation potential were optimized. The obtained LOD of 1.6×10−11 M is the lowest LOD, compared to LODs reported in the literature [31] for detection of ETO, using electrochemical techniques. This method was successfully applied for direct determination of ETO, and tested for human blood sample with high specificity, and sensitivity.