Electrochemical biosensors for DNA hybridization and DNA damage

Paleček, Emil; Fojta, Miroslav; Tomschik, Miroslav; Wang, Joseph

doi:10.1016/s0956-5663(98)00017-7

Cited by 274 publications

(148 citation statements)

References 37 publications

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“…For impedance studies, a sine wave with 10 mV of amplitude was applied to the electrode over the formal potential of the Fe(CN) 6 3-/4-redox couple (0.2 V). Impedance spectra were recorded in the frequency range varying from 100 kHz to 100 mHz.…”

Section: Impedance Measurementsmentioning

confidence: 99%

“…Impedance spectra were recorded in the frequency range varying from 100 kHz to 100 mHz. Electrochemical impedance spectra were fitted using an equivalent circuit contained on the FRA software AUTOLAB (Eco Chemie, Netherlands) and the resistance of charge transfer (R ct ) values were determined in a pH 4.3 acetate buffer using 5 mmol L -1 of Fe (CN) 6 3-/4-as redox probe.…”

Section: Impedance Measurementsmentioning

confidence: 99%

“…Recently, electrochemical sensors have been developed for the rapid detection of DNA damage. [4][5][6][7] Such devices are being successfully used in food industry, environmental fields and clinical chemistry. Biosensors for such analysis can be prepared with multicomponent films of polyions and other charged materials and to achieve high specificity, high sensitivity, rapid response and flexibility of use, it is clear that the research continues to focus on new assembly strategies.…”

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confidence: 99%

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Kinetic studies of HRP adsorption on ds-DNA immobilized on gold electrode surface by EIS and SPR

Ferreira

Kubota

2010

J. Braz. Chem. Soc.

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Este trabalho descreve a construção de um filme de multicomponentes via método layer-bylayer (LbL) e a análise cinética da interação da enzima peroxidase de raiz forte (HRP) sobre a camada de ds-DNA de timo de bezerro formada numa superfície de ouro modificada. As técnicas de ressonância de plásmon de superfície (SPR) e a espectroscopia de impedância eletroquímica (EIE) foram usadas para caracterizar a construção do filme sobre a superfície de ouro. A ressonância de plásmon de superfície forneceu informações essenciais para o estudo e caracterização da interação proteína e ácido nucléico. Este método não necessita de marcador e monitora as interações em tempo real. Os estudos cinéticos obtidos por SPR da formação do filme de HRP sobre o ds-DNA apresentaram valores de 24,7 L mol -1 s -1 e 1,2×10 -3 s -1 para k a e k d , respectivamente. A energia livre de Gibbs obtida para o sistema foi de -23,1 kJ mol -1. Os resultados obtidos mostram que a interação das moléculas da enzima sobre o ds-DNA é cinética e termodinamicamente favoráveis e a interação entre as camadas ocorre principalmente pela atração de cargas opostas. This paper describes the construction of a multicomponent film via layer-by-layer (LbL) method and the kinetic analysis of the interaction between horseradish peroxidase (HRP) enzyme on calf thymus double-stranded DNA layer on a modified gold surface. Surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) are used to characterize the successful construction of the film on the gold surface. Surface plasmon resonance provided essential information for the study and characterization of protein and nucleic acid interaction and this method is label-free and monitors the interactions in real time. The kinetic studies determined by SPR of the horseradish peroxidase film formation on ds-DNA layer showed values of 24.7 L mol -1 s -1 and 1.2×10 -3 s -1 for k a and k d , respectively. The Gibbs free energy obtained for the system was -23.1 kJ mol -1. The results obtained show that the interaction of the enzymes molecules on ds-DNA is kinetically and thermodynamically favourable and the interaction among the layers probably occurs mainly by attraction of opposite charges.Keywords: SPR, EIS, kinetic adsorption, DNA, HRP, protein-DNA interaction IntroductionThe interaction of nucleic acids with protein has been a primary area of interest for therapeutic development and in the study of cellular processes.1-3 Development of devices based on this interaction presents application in the investigations of the toxicity pathways of nucleic acid. Recently, electrochemical sensors have been developed for the rapid detection of DNA damage. [4][5][6][7] Such devices are being successfully used in food industry, environmental fields and clinical chemistry. Biosensors for such analysis can be prepared with multicomponent films of polyions and other charged materials and to achieve high specificity, high sensitivity, rapid response and flexibility of use, it is clear that the research continues to focus ...

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Section: Impedance Measurementsmentioning

confidence: 99%

Section: Impedance Measurementsmentioning

confidence: 99%

mentioning

confidence: 99%

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Kinetic studies of HRP adsorption on ds-DNA immobilized on gold electrode surface by EIS and SPR

Ferreira

Kubota

2010

J. Braz. Chem. Soc.

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“…For instance, DNA damage was investigated with electrochemical and electrochemiluminescent sensors and sensor arrays by a number of research groups [10][11][12][13][14][15]. We also developed a photoelectrochemical DNA sensor for the rapid detection of chemical-induced DNA damage [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Development of microplate-based photoelectrochemical DNA biosensor array for high throughput detection of DNA damage

Liu

Jia

Guo

2012

Sensors and Actuators B: Chemical

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“…Electrochemical signal transduction appears to be a useful alternative to the optical one mainly due to lower costs and easier construction of simple, portable, perhaps one-purpose devices. It is expected that electrochemical sensors for DNA hybridization or DNA damage (reviewed in [1,5,6]) will be available in near future for routine medical diagnostics, for environmental analysis, etc.…”

Section: Introductionmentioning

confidence: 99%

Two‐Surface Strategy in Electrochemical DNA Hybridization Assays: Detection of Osmium‐Labeled Target DNA at Carbon Electrodes

et al. 2003

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Target DNAs, including a 71-mer oligonucleotide, a PCR product and a plasmid DNA, all containing oligo(A) stretches, were hybridized at magnetic Dynabeads oligo(dT) 25 (DBT). The hybridization events were detected using a technique based on chemical modification of the target DNA with a complex of osmium tetroxide with 2,2'-bipyridine (Os, bipy) and voltammetric detection at carbon electrodes. DNA was modified with Os, bipy prior to capture at DBT, at the beads, or after release from the beads. In the latter case, DNA-Os, bipy was detected in the reaction mixture using adsorptive transfer stripping voltammetry involving extraction of unreacted Os, bipy from the electrode by organic solvents. Pre-labeling of the target plasmid DNA and the PCR product with Os, bipy significantly increased the yield of DNA captured at the beads. Tens of femtomoles of both short (the 71-mer oligonucleotide) and long (the 3-kilobase plasmid) target DNAs in a 20-microliter hybridization sample can be easily detected by means of these techniques. Various carbon electrode materials, including pyrolytic graphite (PGE), highly oriented pyrolytic graphite (HOPGE), carbon paste (CPE), glassy carbon and pencil graphite, were tested regarding their suitability for the detection of osmium-labeled DNA. Among them, PGE and HOPGE appeared usable in the measurements of both purified DNA-Os, bipy and its mixtures with unreacted Os, bipy while CPE was suitable for the detection purified osmium-labeled DNA.

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Electrochemical biosensors for DNA hybridization and DNA damage

Cited by 274 publications

References 37 publications

Kinetic studies of HRP adsorption on ds-DNA immobilized on gold electrode surface by EIS and SPR

Kinetic studies of HRP adsorption on ds-DNA immobilized on gold electrode surface by EIS and SPR

Development of microplate-based photoelectrochemical DNA biosensor array for high throughput detection of DNA damage

Two‐Surface Strategy in Electrochemical DNA Hybridization Assays: Detection of Osmium‐Labeled Target DNA at Carbon Electrodes

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