Electrochemical Detection of Pathogen Infection Using Cell Chip

Abstract

The immobilized cell using self-assembled synthetic oligopeptide was applied to the electrochemical detection of pathogen infection. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for the immobilization of HeLa cell on gold (Au) substrate. Layer formation and immobilization of the cell were investigated with surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS). Experimental results showed that the thin film of cysteine-terminated synthetic oligopeptide was successfully fabricated and it could be applied for the immobilization of HeLa cells. The adhered living cell was exposed to E. coli O157:H7, which induced the change of SPR angle and electrochemical impedance signal. The proposed cell immobilization method using self-assembly technique can be applied to construct the cell microarray for on-site pathogen monitoring.

P7 - Optical Immune Biosensor Based on SPR for the Detection of Salmonella Typhimurium

“…3 CFU per test portion (Table). After all it is necessary to emphasize that recently an SPR method is widely used for the detection of pathogenic microorganisms [14][15][16][17][18][19][20]. As in ELISA, SPR detection is quick and cheap.…”

Impedance interrogated affinity biosensors for medical applications: novel targets and mechanistic studies

“…This was then used to successfully detect specifi c antibodies against FMDV at 8.7 μg mL −1 . Synthetic oligopeptides have since been immobilised onto gold to bind mammalian HeLa cells to detect enterohaemorrhagic E. coli O157:H7 (Choi et al , 2007). Although successful detection was obtained, the results were not quantitative.…”

Nanostructured surfaces for analysis of anticancer drug and cell diagnosis based on electrochemical and SERS tools

“…In addition, their applications for the discovery of new anticancer drugs by monitoring the changes in cell behavior that are induced by anticancer drugs were based on the results that change in the electrochemical response of treated cells [ 29 – 31 ]. Different electrochemical techniques were used, including impedance spectroscopy (EIS) [ 15 , 17 ], amperometry, electric cell-substrate impedance sensing (ECIS) [ 32 , 33 ], cyclic voltammetry (CV) [ 16 , 34 – 38 ], differential pulse voltammetry (DPV) [ 39 , 40 ], open circuit potential at the cell/sensor interface [ 30 ], and scanning electrochemical microscopy (SECM) [ 27 , 41 , 42 ]. Raman spectroscopy is one of the most promising label-free rapid and nondestructive techniques for cancer diagnosis, in situ monitoring of the effects, action mechanisms, and distribution and metabolism of different drugs at the cellular level without any sample preparations, which could reduce the need for animal experiments.…”