Electrochemical detection of telomerase in cancer cells based on the in-situ formation of streptavidin-biotin-DNA-biotin networks for signal amplification

Liu, Lin; Wu, Daohong; Zhen, Shuang; Lu, Kaiqing; Yi, Xinyao; Sun, Zhifang

doi:10.1016/j.snb.2021.129659

Cited by 21 publications

(14 citation statements)

References 70 publications

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“…In this study, graphene oxide (GO) and AuNPs were used as electroactive materials for the signal amplification of the sensor. In order to improve the sensitivity of the immunosensor, a biotin–streptavidin (SA) system was adopted ( Xia et al, 2020 ; Liu et al, 2021a ; Liu et al, 2021b ; Xia et al, 2021 ). The biotin-SA CEA antibody (Bio-Ab) was captured because of the high affinity between biotin and SA.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide

Guo

2022

Front. Chem.

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Tumor markers are one of the important indicators for early cancer diagnosis. As a new analytical method, electrochemical immunosensing analysis has the advantages of high sensitivity, good selectivity, and rapid detection, which is of great significance for the detection of tumor markers. In this work, an AuNP/reduced graphene oxide (AuNP/rGO) composite was synthesized. We used it for electrochemical sensor fabrication with the assistance of the biotin–streptavidin protein (SA) system to further amplify the signal to achieve sensitive detection of carcinoembryonic antigen (CEA). In addition, AuNPs have been incorporated due to their good electrical conductivity and biocompatibility, which can accelerate electron transfer at the electrode interface and improve the loading capacity to capture antibodies. The fabricated AuNPs/SA/rGO has a large working surface area and high material utilization ratio, which improves the catalytic capacity of H2O2 reduction and effectively amplifies the current signal. The linear range of the response current signal of the sensor toward the CEA concentration is 20 fg/ml to 200 ng/ml, and the limit of detection can achieve 6.2 fg/ml. In addition, the fabricated immunosensor has good reproducibility, selectivity, and stability.

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Section: Introductionmentioning

confidence: 99%

Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide

Guo

2022

Front. Chem.

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“…as proposed by Gooding´s Group, [7,21,22,45] and for amplification using more conventional electrochemical biosensor formats. [23,24] The key to "dispersible electrodes" is the use of Au-coated MNPs (Au@MNPs), modified with the appropriate receptor to selectively recognize the analyte, combining the ability to move the NPs with a magnetic field with electrical conductivity. This strategy showed a 1000-fold improvement in both detection limit and response time compared to the same sensing elements in macroscopic and planar electrochemical sensors.…”

Section: Dna Network and Hydrogelsmentioning

confidence: 99%

“…DNA networks generated by DNA probes functionalized with biotin at both ends [23] or by non-linear HCR amplification strategies [24,46] (Figure 6b) have been exploited to develop biosensors with improved sensitivity. Representative examples of [22] Copyright 2021, RSC; (b) Reproduced with permission.…”

Section: Dna Network and Hydrogelsmentioning

confidence: 99%

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

Campuzano

Pedrero

Barderas

et al. 2022

Adv Materials Technologies

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Electrochemical biosensors continue to evolve at an astonishing pace, consolidating as competitive tools for determining a wide range of targets and relentlessly strengthening their attributes in terms of sensitivity, selectivity, simplicity, response time, and antifouling ability, making them suitable for getting a foothold in real‐world applications. The design and exploitation of nanostructured or multifunctional nucleic acid or peptide biomaterials are playing a determinant role in these achievements. With the aim of highlighting the potential and opportunities of these biomaterials, this perspective article critically discusses and overviews the electrochemical biosensors reported since 2019 involving nanostructured and multifunctional DNA biomaterials, multifunctional aptamers, modern peptides, and CRISPR/Cas systems. The use of these biomaterials as recognition elements, electrode modifiers (acting as linkers or creating scaffolds with antifouling properties), enzyme substrates, and labeling/carrier agents for signal amplification is discussed through rationally and strategically selected examples, concluding with a personal perspective about the challenges to be faced and future lines of action.

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“…Then, the resultant solution was incubated with the peptide substrate. For real sample assays, HeLa cells were cultured and the cell lysates were extracted with our reported procedures [30,31]. Then, the peptide substrate was incubated with the diluted cell lysates to react for 2 h. Finally, the reaction mixture was delivered to the flow cell, followed by injection of SA-IgG to the channel after the baseline stable was attained.…”

Section: Inhibitor Detection and Cell Lysate Analysismentioning

confidence: 99%

Surface Plasmon Resonance for Protease Detection by Integration of Homogeneous Reaction

Xia

Liu

2021

Biosensors

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The heterogeneous assays of proteases usually require the immobilization of peptide substrates on the solid surface for enzymatic hydrolysis reactions. However, immobilization of peptides on the solid surface may cause a steric hindrance to prevent the interaction between the substrate and the active center of protease, thus limiting the enzymatic cleavage of the peptide. In this work, we reported a heterogeneous surface plasmon resonance (SPR) method for protease detection by integration of homogeneous reaction. The sensitivity was enhanced by the signal amplification of streptavidin (SA)-conjugated immunoglobulin G (SA-IgG). Caspase-3 (Cas-3) was determined as the model. A peptide labeled with two biotin tags at the N- and C-terminals (bio-GDEVDGK-bio) was used as the substrate. In the absence of Cas-3, the substrate peptide was captured by neutravidin (NA)-covered SPR chip to facilitate the attachment of SA-IgG by the avidin-biotin interaction. However, once the peptide substrate was digested by Cas-3 in the aqueous phase, the products of bio-GDEVD and GK-bio would compete with the substrate to bond NA on the chip surface, thus limiting the attachment of SA-IgG. The method integrated the advantages of both heterogeneous and homogeneous assays and has been used to determine Cas-3 inhibitor and evaluate cell apoptosis with satisfactory results.

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Electrochemical detection of telomerase in cancer cells based on the in-situ formation of streptavidin-biotin-DNA-biotin networks for signal amplification

Cited by 21 publications

References 70 publications

Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide

Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide

Empowering Electrochemical Biosensing through Nanostructured or Multifunctional Nucleic Acid or Peptide Biomaterials

Surface Plasmon Resonance for Protease Detection by Integration of Homogeneous Reaction

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