Electrochemical Determination of the KRAS Genetic Marker for Colon Cancer with Modified Graphete and Graphene Paste Electrodes

Al-Ogaidi, Ahmed Jassim Muklive; Staden, Raluca‐Ioana Stefan‐van; Gugoaşă, Livia Alexandra; Roşu, Marcela‐Corina; Socaci, Crina

doi:10.1080/00032719.2018.1453516

Cited by 8 publications

(6 citation statements)

References 22 publications

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“…Al-Ogaidi et al used the conjugate of substituted zinc(II) phthalocyanine and BODIPY dye (Figure 9) for fabrication of an electrochemical sensor based on graphite paste. The determination of KRAS, a genetic marker of colon cancer, was performed in comparison to two electrodes modified with graphene oxide/TiO 2 and azulenes [132].…”

Section: Other Biomarkers (Nadh Ascorbic Acid Uric Acid and Urea Nucleic Acids And Nucleotides)mentioning

confidence: 99%

“…an electrochemical sensor based on graphite paste. The determination of KRAS, a genetic marker of colon cancer, was performed in comparison to two electrodes modified with graphene oxide/TiO2 and azulenes [132]. A different type of carbon-based nanomaterial-graphene-like carbon nitride (g-C3N4)-was used by Dai et al in the photoelectrochemical determination of choline.…”

Section: Other Biomarkers (Nadh Ascorbic Acid Uric Acid and Urea Nucleic Acids And Nucleotides)mentioning

confidence: 99%

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Azaporphyrins Embedded on Carbon-Based Nanomaterials for Potential Use in Electrochemical Sensing—A Review

2021

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Phthalocyanines and porphyrazines as macrocyclic aza-analogues of well-known porphyrins were deposited on diverse carbon-based nanomaterials and investigated as sensing devices. The extended π-conjugated electron system of these macrocycles influences their ability to create stable hybrid systems with graphene or carbon nanotubes commonly based on π–π stacking interactions. During a 15-year period, the electrodes modified by deposition of these systems have been applied for the determination of diverse analytes, such as food pollutants, heavy metals, catecholamines, thiols, glucose, peroxides, some active pharmaceutical ingredients, and poisonous gases. These procedures have also taken place, on occasion, in the presence of various polymers, ionic liquids, and other moieties. In the review, studies are presented that were performed for sensing purposes, involving azaporphyrins embedded on graphene, graphene oxide or carbon nanotubes (both single and multi-walled ones). Moreover, possible methods of electrode fabrication, limits of detection of each analyte, as well as examples of macrocyclic compounds applied as sensing materials, are critically discussed.

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Section: Other Biomarkers (Nadh Ascorbic Acid Uric Acid and Urea Nucleic Acids And Nucleotides)mentioning

confidence: 99%

Section: Other Biomarkers (Nadh Ascorbic Acid Uric Acid and Urea Nucleic Acids And Nucleotides)mentioning

confidence: 99%

Azaporphyrins Embedded on Carbon-Based Nanomaterials for Potential Use in Electrochemical Sensing—A Review

2021

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“…The developed biosensor was tested in SW480 CRC cell lysates, which had results that were consistent with those from the analysis of the High-Resolution Melt (HRM) curve after amplification by PCR. Ahmed Jassim Muklive Al-Ogaidia and co-workers [100] reported voltammetric biosensors based on carbon matrices modified with either phthalocyanine-boron dipyrromethene (BODIPY) dye or azulene (A1: 2,6-bis((E)-2-(furan-2-yl)vinyl)-4-(4,6,8-trimethylazulen-1-yl)pyridine and A2: 2,6-bis((E)-2-(thiophen-3-yl)vinyl)-4-(4,6,8-trimethylazulen-1-yl)pyridine) composites as working electrode-based carbon matrices. The biosensors based on phthalocyanine-BODIPY, A1/PtTiO 2 -reduced graphene oxide, and A2/PtTiO 2 -reduced graphene oxide detected 2.06 × 10 −6 , 8.67 × 10 −10 , and 2.94 × 10 −5 µg mL −1 of the KRAS gene in a linear concentration range from 1.54 × 10 −4 to 1.92 × 10 −2 , from 3.07 × 10 −7 to 3.84 × 10 −3 , and from 3.84 × 10 −8 to 0.48 µg mL −1 , respectively.…”

Section: Electrochemical Biosensing Of Nucleic Acid Biomarkers Of Crcmentioning

confidence: 99%

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

et al. 2020

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The accurate determination of specific tumor markers associated with cancer with non-invasive or minimally invasive procedures is the most promising approach to improve the long-term survival of cancer patients and fight against the high incidence and mortality of this disease. Quantification of biomarkers at different stages of the disease can lead to an appropriate and instantaneous therapeutic action. In this context, the determination of biomarkers by electrochemical biosensors is at the forefront of cancer diagnosis research because of their unique features such as their versatility, fast response, accurate quantification, and amenability for multiplexing and miniaturization. In this review, after briefly discussing the relevant aspects and current challenges in the determination of colorectal tumor markers, it will critically summarize the development of electrochemical biosensors to date to this aim, highlighting the enormous potential of these devices to be incorporated into the clinical practice. Finally, it will focus on the remaining challenges and opportunities to bring electrochemical biosensors to the point-of-care testing.

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“…For protein biomarker detection, graphene-based electrochemical immunosensors have been developed for the sensitive detection of common cancer proteins like prostate specific antigen (PSA) [ 30 ], carcinoembryonic antigen (CEA) [ 31 ], and cancer antigen 125 (CA125) in clinical samples [ 32 ]. Graphene-modified electrochemical genosensors have demonstrated capabilities for detecting cancer-related DNA mutations such as in BRAF [ 33 ], KRAS [ 34 ], and EGFR [ 35 ] genes in liquid biopsy and patient tissue samples [ 36 ]. Aptamer-based graphene biosensors have shown promise in the detection of cancer biomarkers like vascular endothelial growth factor (VEGF) [ 37 ] and thrombin [ 38 ] down to picomolar levels.…”

Section: Introductionmentioning

confidence: 99%

Strategies and Applications of Graphene and Its Derivatives-Based Electrochemical Sensors in Cancer Diagnosis

Fu,

Zheng,

et al. 2023

Molecules

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Graphene is an emerging nanomaterial increasingly being used in electrochemical biosensing applications owing to its high surface area, excellent conductivity, ease of functionalization, and superior electrocatalytic properties compared to other carbon-based electrodes and nanomaterials, enabling faster electron transfer kinetics and higher sensitivity. Graphene electrochemical biosensors may have the potential to enable the rapid, sensitive, and low-cost detection of cancer biomarkers. This paper reviews early-stage research and proof-of-concept studies on the development of graphene electrochemical biosensors for potential future cancer diagnostic applications. Various graphene synthesis methods are outlined along with common functionalization approaches using polymers, biomolecules, nanomaterials, and synthetic chemistry to facilitate the immobilization of recognition elements and improve performance. Major sensor configurations including graphene field-effect transistors, graphene modified electrodes and nanocomposites, and 3D graphene networks are highlighted along with their principles of operation, advantages, and biosensing capabilities. Strategies for the immobilization of biorecognition elements like antibodies, aptamers, peptides, and DNA/RNA probes onto graphene platforms to impart target specificity are summarized. The use of nanomaterial labels, hybrid nanocomposites with graphene, and chemical modification for signal enhancement are also discussed. Examples are provided to illustrate applications for the sensitive electrochemical detection of a broad range of cancer biomarkers including proteins, circulating tumor cells, DNA mutations, non-coding RNAs like miRNA, metabolites, and glycoproteins. Current challenges and future opportunities are elucidated to guide ongoing efforts towards transitioning graphene biosensors from promising research lab tools into mainstream clinical practice. Continued research addressing issues with reproducibility, stability, selectivity, integration, clinical validation, and regulatory approval could enable wider adoption. Overall, graphene electrochemical biosensors present powerful and versatile platforms for cancer diagnosis at the point of care.

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Electrochemical Determination of the KRAS Genetic Marker for Colon Cancer with Modified Graphete and Graphene Paste Electrodes

Cited by 8 publications

References 22 publications

Azaporphyrins Embedded on Carbon-Based Nanomaterials for Potential Use in Electrochemical Sensing—A Review

Azaporphyrins Embedded on Carbon-Based Nanomaterials for Potential Use in Electrochemical Sensing—A Review

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

Strategies and Applications of Graphene and Its Derivatives-Based Electrochemical Sensors in Cancer Diagnosis

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