Electrode modifications for electrochemical sensors attract a lot of attention every year. Among them, hydrogels are a relatively special class of electrode modifier. Since hydrogels often contain polymers, even though they are conductive polymers, they are not ideal electrode modifiers because of their poor conductivity. However, the micro-aqueous environment and the three-dimensional structure of hydrogels are an excellent platform for immobilizing bioactive molecules and maintaining their activity. This gives the hydrogel-modified electrochemical sensor the potential to perform specific recognition. At the same time, the rapid development of nanomaterials also makes the composite hydrogel have good electrical conductivity. This has led many scientists to become interested in hydrogel-based electrochemical sensors. In this review, we summarize the development process of hydrogel-based electrochemical sensors, starting from 2000. Hydrogel-based electrochemical sensors were initially used only as a carrier for biomolecules, mostly for loading enzymes and for specific recognition. With the widespread use of noble metal nanoparticles and carbon materials, hydrogels can now be used to prepare enzyme-free sensors. Although there are some sporadic studies on the use of hydrogels for practical applications, the vast majority of reports are still limited to the detection of common model molecules, such as glucose and H2O2. In the review, we classify hydrogels according to their different conducting strategies, and present the current status of the application of different hydrogels in electrochemical sensors. We also summarize the advantages and shortcomings of hydrogel-based electrochemical sensors. In addition, future prospects regarding hydrogel for electrochemical sensor use have been provided at the end.