Electrochemical immunosensor detection of antigliadin antibodies from real human serum

Rosales-Rivera, Luis Carlos; Acero-Sánchez, Josep Lluís; Lozano-Sánchez, Pablo; Katakis, Ioanis; O’Sullivan, Ciara K.

doi:10.1016/j.bios.2011.05.004

Cited by 43 publications

(20 citation statements)

References 39 publications

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“…Dulay, Lozano‐Sánchez, Iwuoha, Katakis, and O’Sullivan () continued the work of Rosales‐Rivera et al () for the development of an ECIS for the detection of TGA. The instrumentation and electrochemical setup were similar to as adapted for AGA detection (Rosales‐Rivera et al, ). The ECIS was compared with ELISA indicating a good correlation.…”

Section: Biosensors For CD Diagnosismentioning

confidence: 95%

“…An immunosensor was also developed (Rosales‐Rivera, Acero‐Sánchez, Lozano‐Sánchez, Katakis, & O’Sullivan, ) using a similar principle (Balkenhohl & Lisdat, ). The antigen was immobilized on the gold surface using a carboxylic‐ended bipodal alkanethiol, which was covalently linked with amino groups of the antigen protein.…”

Section: Biosensors For CD Diagnosismentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in biosensors for diagnosis of celiac disease: A review

Gupta

Kaushal

Kumar

et al. 2018

Biotech & Bioengineering

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Celiac disease (CD) is an intestinal issue activated by the inappropriate immune reaction towards gluten protein of wheat, rye, barley, oats, and autoantigen, tissue transglutaminase. Regardless of the accessibility of immunochemical conventions for research facility analysis of CD, there is as yet a need of speedier, less expensive, and simpler devices for diagnosing CD. This review concentrates on progresses in biosensors for diagnosing CD in perspective of the scaled down hardware, multianalyte discovery and low sample volume necessity. Various recently developed biosensors in this field are presented.

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Section: Biosensors For CD Diagnosismentioning

confidence: 95%

Section: Biosensors For CD Diagnosismentioning

confidence: 99%

Recent advances in biosensors for diagnosis of celiac disease: A review

Gupta

Kaushal

Kumar

et al. 2018

Biotech & Bioengineering

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“…In relation to the detection of gliadin autoantibodies, although being a less specific biomarker for CD diagnosis by serological tests, some electrochemical immunosensors for the detection of antigliadin antibodies have been reported. For instance, Rosales et al developed an EI using gold electrodes with oriented covalent modification of gliadin on the electrode surface [16]. The amperometric detection was also conducted using the enzymatic system HRP-TMB.…”

Section: Electrochemical Immunosensors For the Detection Of Celiac DImentioning

confidence: 99%

Electrochemical Immunosensors for Celiac Disease Detection

Martín‐Yerga¹,

Costa-Garcı́a

2016

IJCD

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“…Recently, several electrochemical assays for the detection of auto‐antibodies and genetic markers associated with celiac disease have been developed by one of our groups. Highly stable and electron permeable self‐assembled monolayers of a carboxylic acid terminated dithiol scaffold on gold or supramolecular polymeric assemblies were used to covalently link tTG , digested gliadin , or IgA to develop sandwich‐type amperometric immunosensors to detect the corresponding autoantibodies in serum samples of celiac patients with low LODs and the results were in all cases compared with an standard ELISA with excellent correlation. In addition, we have reported a multiplexed electrochemical genosensor on ten probes specific to DQA1 and DQB1 alleles immobilised on an array of 36 electrodes.…”

Section: Introductionmentioning

confidence: 99%

Bleed‐to‐read disposable microsystems for the genetic and serological analysis of celiac disease markers with amperometric detection

et al. 2015

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Celiac disease is an auto-immune disorder induced by ingestion of gluten in genetically predisposed individuals. Its diagnostics is more accurate using a combination of immunologic and genetic tests to detect of high levels of certain auto-antibodies and the presence human leukocyte antigen HLA-DQ2 or HLA-DQ8 genetic markers. In this work, we report the design and testing of automated microsystems combining sample treatment, storage, fluidic transport, and detection in a single platform able to carry out genetic or serologic analysis for detection of celiac disease markers. These microsystems share a common footprint and many fluidic features and are thus able to perform a complete assay. The microsystem for the genetic assay extracts and amplifies the DNA prior to detection, while the serology microsystem contains a filter and chamber for the generation and subsequent dilution of plasma. The performance of both platforms is demonstrated and compared with reference methods with an excellent correlation, which makes the developed platform amenable for clinical studies.

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Electrochemical immunosensor detection of antigliadin antibodies from real human serum

Cited by 43 publications

References 39 publications

Recent advances in biosensors for diagnosis of celiac disease: A review

Recent advances in biosensors for diagnosis of celiac disease: A review

Electrochemical Immunosensors for Celiac Disease Detection

Bleed‐to‐read disposable microsystems for the genetic and serological analysis of celiac disease markers with amperometric detection

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