Electrochemical immunosensor for determination of aflatoxin B1 in barley

Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detection

“…During the past two decades spectrophotometric ELISAs specific for AFB 1 (Dutta and Das, 2000;Kolosova et al, 2006), total aflatoxins (Ayciek et al, 2005;Zheng et al, 2005) and AFM 1 (Yaroglu et al, 2005;Rastogi et al, 2004) have been developed and their simplicity, adaptability and sensitivity have been demonstrated. To achieve higher sensitivity and move to the use of disposable probes, electrochemical immunosensors based on single screen-printed electrodes have also been recently proposed by our group for the detection of AFB 1 in barley (Ammida et al, 2004) and for AFM 1 in milk (Micheli et al, 2005).…”

“…The indirect ELISA was performed according to the procedure described in Ammida et al (2004), with minor modifications such as in the characteristic of specific antibody used during the competition step. The plate was coated with AFB 1 -BSA conjugate (1 g/mL, 50 L/well) in CB, pH 9.6, overnight at 4 • C, then blocked with 1% PVA (50 L/well) for 1 h at room temperature.…”

Aflatoxin Measurement and Analysis

“…Finally, the binding enzyme density can be shown by current produced from the catalytic oxidation reaction of the enzyme with substrates. Many such immunoassays have been described for aflatoxins (Ammida et al, 2004;Micheli et al, 2005;Parker & Tothill, 2009;Tan et al, 2009;Vig, et al, 2009) and they all had high sensitivities (LOD 0.01-0.4 ng/mL). With a non-competitive immunoassay, the formation of the Ab-Ag complex by a simple one-step immunoreaction between the immobilized enzyme-Ab conjugate and analytes in sample solution introduced a barrier of direct electrical communication between the immobilized enzyme and the electrode surface, so local current variations could be detected by the enzyme bioelectrocatalytic oxidation reaction with substrates.…”

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

“…The presented method was successfully applied for detection of AFB1 in barley samples with high sensitivity and good recoveries. The SPE-based ELISA showed better analytical performance than spectrophotometric ELISA with a LOD of 30 pg/mL [49]. After one year, a direct HRP-linked chronoamperometric immunosensor was developed for detection of AFM1 in milk samples [50].…”