Electrochemical Immunosensor for the Determination of Total Ghrelin Hormone in Saliva

Pérez‐Quintanilla, Damián; Serafín, Verónica; Agüı́, L.; Yáñez‐Sedeño, Paloma; Pingarrón, José M.

doi:10.1002/elan.201400662

Cited by 14 publications

(2 citation statements)

References 24 publications

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“…Electrochemical immunosensors have been extensively used for the determination of diagnostics biomarkers because of the interesting advantages they offer in terms of inherent high sensitivity and selectivity, great precision and accuracy, low cost, minimum sample requirements, simplicity of operation and possible integration into compact analytical devices as well as demonstrated suitability for clinical analysis [33,34,35,36,37,38,39,40]. In the following sections, the most interesting characteristics of selected approaches using carbon nanomaterials as modifiers of electrochemical immunosensing platforms will be briefly discussed.…”

Section: Electrochemical Immunosensors Involving Carbon Nanomaterimentioning

confidence: 99%

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

2016

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Early diagnosis is often the key to successful patient treatment and survival. The identification of various disease signaling biomarkers which reliably reflect normal and disease states in humans in biological fluids explain the burgeoning research field in developing new methodologies able to determine the target biomarkers in complex biological samples with the required sensitivity and selectivity and in a simple and rapid way. The unique advantages offered by electrochemical sensors together with the availability of high affinity and specific bioreceptors and their great capabilities in terms of sensitivity and stability imparted by nanostructuring the electrode surface with different carbon nanomaterials have led to the development of new electrochemical biosensing strategies that have flourished as interesting alternatives to conventional methodologies for clinical diagnostics. This paper briefly reviews the advantages of using carbon nanostructures and their hybrid nanocomposites as electrode modifiers to construct efficient electrochemical sensing platforms for diagnosis. The review provides an updated overview of some selected examples involving attractive amplification and biosensing approaches which have been applied to the determination of relevant genetic and protein diagnostics biomarkers.

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Section: Electrochemical Immunosensors Involving Carbon Nanomaterimentioning

confidence: 99%

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

2016

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“…An electrochemical immunosensor for ghrelin (GHRL) determination was also developed by using a direct competitive format involving GHRL and biotinylated-GHRL, Strep-AP as enzymatic tracer and ProtG-MMPs as solid support [34]. By measuring the DPV signal of 1-naphtol produced upon 1-naphtyl phosphate addition at SPCEs, a calibration plot with a linear range between 10 −3 and 10 3 ng·mL −1 and a LOD value of 7 pg·mL −1 (much smaller than those provided by commercial ELISAs) were achieved.…”

Section: Micromagnetic Particlesmentioning

confidence: 99%

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Yáñez‐Sedeño

Campuzano

Pingarrón

2016

Sensors

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Ultrasensitive biosensing is currently a growing demand that has led to the development of numerous strategies for signal amplification. In this context, the unique properties of magnetic particles; both of nano- and micro-size dimensions; have proved to be promising materials to be coupled with disposable electrodes for the design of cost-effective electrochemical affinity biosensing platforms. This review addresses, through discussion of selected examples, the way that nano- and micro-magnetic particles (MNPs and MMPs; respectively) have contributed significantly to the development of electrochemical affinity biosensors, including immuno-, DNA, aptamer and other affinity modes. Different aspects such as type of magnetic particles, assay formats, detection techniques, sensitivity, applicability and other relevant characteristics are discussed. Research opportunities and future development trends in this field are also considered.

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Proximity Ligation Assay‐induced Structure‐switching Hairpin DNA toward Development of Electrochemical Immunosensor

Tang

2016

Electroanalysis

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As imple and feasible method to detect and quantify lowconcentrationb iomolecules is very important in the fields of food safety and environmental monitoring [1].R ecent ongoinge ffort has been made worldwide to expend the assay development, particularly toward immunoassay,t o simplifyt he assay process with the aim of manufacturing portable and affordabled evices [2].E lectrochemical immunosensorsh ave attracted considerable attention over the past few years due to their intrinsic advantages, such as good portability,l ow cost, simple instrumentation and high sensitivity [3].U ndoubtedly,e nzyme-linked immunosorbent assay (ELISA) has emerged as the methodo f choice for this purpose.U nfortunately, conventional electrochemical enzyme immunosensors are often involved in multiple-step reactions,s eparation and washingp rocesses [4].M oreover, enzyme immunoassays are usually susceptible to interference and changes in the assayc onditions during the signal generations tage [4a].A na lternative immunosensing strategy that is basedo na ne lectrochemical principle and does not require the sample separation and the enzymatic participation would be advantageous.Thee mergence of target-induced signal switch (ON$OFF) opens an ew horizon for rapid detection of analytew ithout the need of sample separation [5].T he Qin'sg roup designed ap otentiometric aptasensor based on target-induced conformational switcho fD NA probe for the detection of adenosine-5'-triphosphate using ap olymeric membrane silveri on-selectivee lectrode [6]. Plaxco et al. reported DNA-based bioelectrochemical switches for the quantitative detection of antibodies [7]. Dou etal. developed target-induced reconfiguration of DNAp robes for recycling amplification and signal-on electrochemical detection of hereditary tyrosineminia type Ig ene [8].T ot he best of our knowledge,t here is no report focusing on DNA-based conformational switches for the development of electrochemical immunosensors. To this end, our motivation of this work is to explore an ovel target-induced DNAs ignal switch for the constructiono fe lectrochemical immunosensor.Ty pically,t his type of methodology relies on devising ad etection principle that is modulated and either turned on or turned off as ar esult of binding reaction. Proximity immunoassays, e.g.,p roximity ligation assay( PLA), can Abstract:Anovel electrochemicali mmunosensor was designed for sensitive detection of mycotoxins (aflatoxin B 1 , AFB 1 ,u sed in this case) by couplingp roximity ligation assay-induced conformations witch of hairpin DNAw ith the antigen-antibody reaction.T he assay was carriedo ut by anti-AFB 1 antibody-conjugatedD NA 1 (mAb-DNA 1 ), AFB 1 -BSA-labeled DNA 2 (AFB 1 -DNA 2 )a nd hairpin DNA. Each hairpin includedastem of 6b ase pairsa nd a6n ucleotide( nt) loop with the labelled ferrocene tag at the 3' end, which was immobilizedo nt he electrode via self-assembly of the terminal thiolm oiety at the 5' end. Thep roximity ligation assay was carried out via the specific antigen-antibody reactionb etween ...

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Electrochemical Immunosensor for the Determination of Total Ghrelin Hormone in Saliva

Cited by 14 publications

References 24 publications

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Proximity Ligation Assay‐induced Structure‐switching Hairpin DNA toward Development of Electrochemical Immunosensor

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