Electrochemical immunosensor for ultrasensitive detection of microcystin-LR based on graphene–gold nanocomposite/functional conducting polymer/gold nanoparticle/ionic liquid composite film with electrodeposition

Ruiyi, Li; Qianfang, Xia; Li, Zaijun; Sun, Xiulan; Junkang, Liu

doi:10.1016/j.bios.2013.01.007

Cited by 78 publications

(29 citation statements)

References 38 publications

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“…10 Immunoassays for MCLR detection based on monoclonal or polyclonal antibodies have been extensively studied due to their high sensitivity and specific molecular recognition, without the need for complicated sample pretreatment procedures. Examples include, fluorescent immunoassay, enzyme-linked immunosorbent assay (ELISA) and various immunosensors based on colorimetric, 11 fluorescent, 12,13 chemiluminescent, 14,15 and electrochemical 16,17 signals. The immunoassays are usually carried out by either direct or indirect/competitive format, which involve chemical labeling or modification of small antigen MCLR or antibody with proteins, 18,19 catalytic enzymes, 20−22 or fluorescent dyes.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The immunoassays are usually carried out by either direct or indirect/competitive format, which involve chemical labeling or modification of small antigen MCLR or antibody with proteins, 18,19 catalytic enzymes, 20−22 or fluorescent dyes. 23 Furthermore, nanomaterials, such as carbon nanohorn, 16 carbon nanotube, 20 graphene, 17 TiO 2 nanotube, 24 Au nanoparticles, 11 Ag nanoparticles, 25 and quantum dot (QD), 26 magnetic nanoparticles, 27,28 mesoporous PtRu alloy, 29 were conjugated with antibody as signal transducer and enhancement factors to improve the response speed and sensitivity due to large surface area, and excellent structural, electronic, and optical characteristics. These sensors enabled the quantification of MCLR from μg/L to ng/L levels in tap and ground waters.…”

Section: ■ Introductionmentioning

confidence: 99%

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Label-Free Electrical Immunosensor for Highly Sensitive and Specific Detection of Microcystin-LR in Water Samples

Tan

Saucedo

Ramnani

et al. 2015

Environ. Sci. Technol.

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Microcystin-LR (MCLR) is one of the most commonly detected and toxic cyclic heptapeptide cyanotoxins released by cyanobacterial blooms in surface waters, for which sensitive and specific detection methods are necessary to carry out its recognition and quantification. Here, we present a single-walled carbon nanotube (SWCNTs)-based label-free chemiresistive immunosensor for highly sensitive and specific detection of MCLR in different source waters. MCLR was initially immobilized on SWCNTs modified interdigitated electrode, followed by incubation with monoclonal anti-MCLR antibody. The competitive binding of MCLR in sample solutions induced departure of the antibody from the antibody-antigen complexes formed on SWCNTs, resulting in change in the conductivity between source and drain of the sensor. The displacement assay greatly improved the sensitivity of the sensor compared with direct immunoassay on the same device. The immunosensor exhibited a wide linear response to log value of MCLR concentration ranging from 1 to 1000 ng/L, with a detection limit of 0.6 ng/L. This method showed good reproducibility, stability and recovery. The proposed method provides a powerful tool for rapid and sensitive monitoring of MCLR in environmental samples.

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Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Label-Free Electrical Immunosensor for Highly Sensitive and Specific Detection of Microcystin-LR in Water Samples

Tan

Saucedo

Ramnani

et al. 2015

Environ. Sci. Technol.

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“…Influence of the buffer pH is essential to analytical performance of the immunosensor, because the pH affects not only the electrochemical behavior of the sensor but also the bioactivity of the biomolecules [34]. The pH value of the 0.1 mol L −1 PBS was investigated in the range of 5.0 to 8.0.…”

Section: Resultsmentioning

confidence: 99%

Amino-Functionalization of Carbon Nanotubes by Using a Factorial Design: Human Cardiac Troponin T Immunosensing Application

Freitas

Mattos

Silva

et al. 2014

BioMed Research International

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A simple amino-functionalization method for carbon nanotubes and its application in an electrochemical immunosensor for detection of the human cardiac troponin T are described. Amino-functionalized carbon nanotubes allow oriented antibodies immobilization via their Fc regions, improving the performance of an immunosensor. Herein multiwalled carbon nanotubes were amino-functionalized by using the ethylenediamine reagent and assays were designed by fractional factorial study associated with Doehlert matrix. Structural modifications in the carbon nanotubes were confirmed by Fourier transform infrared spectroscopy. After amino-functionalization the carbon nanotubes were attached to screen-printed carbon electrode and a sandwich-type immunoassay was performed for measuring the cardiac troponin T. The electrochemical measurements were obtained through hydrogen peroxide reaction with peroxidase conjugated to the secondary antibody. Under optimal conditions, troponin T immunosensor was evaluated in serum samples, which showed a broad linear range (0.02 to 0.32 ng mL−1) and a low limit of detection, 0.016 ng mL−1. This amino platform can be properly used as clinical tool for cardiac troponin T detection in the acute myocardial infarction diagnosis.

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“…The synthesized hollow Pt nanoparticles show excellent conductivity. 4. The biosensor could serve as a model for the fabrication of other enzyme sensors.…”

Section: Page21mentioning

confidence: 99%

“…Generally, carbon materials such as graphene [4] and carbon nanotubes [5,6], have been extensively applied to electrochemical biosensors, due to their good conductivity [7] and the proper immobilization of substrates on these species. Among these substrates, graphene oxide (GO), as a derivative of graphene, is hydrophilic due to the presence of many polar functional groups, such as hydroxyl, carbonyl, carboxyl and epoxy groups.…”

Section: Introductionmentioning

confidence: 99%

A sensitive glucose biosensor based on the abundant immobilization of glucose oxidase on hollow Pt nanospheres assembled on graphene oxide–Prussian Blue–PTC-NH2 nanocomposite film

Xu¹,

He²,

Gao³

et al. 2015

Journal of Electroanalytical Chemistry

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Electrochemical immunosensor for ultrasensitive detection of microcystin-LR based on graphene–gold nanocomposite/functional conducting polymer/gold nanoparticle/ionic liquid composite film with electrodeposition

Cited by 78 publications

References 38 publications

Label-Free Electrical Immunosensor for Highly Sensitive and Specific Detection of Microcystin-LR in Water Samples

Label-Free Electrical Immunosensor for Highly Sensitive and Specific Detection of Microcystin-LR in Water Samples

Amino-Functionalization of Carbon Nanotubes by Using a Factorial Design: Human Cardiac Troponin T Immunosensing Application

A sensitive glucose biosensor based on the abundant immobilization of glucose oxidase on hollow Pt nanospheres assembled on graphene oxide–Prussian Blue–PTC-NH2 nanocomposite film

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