Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin

Ojeda, Irene; Moreno‐Guzmán, María; González‐Cortés, Araceli; Yáñez‐Sedeño, Paloma; Pingarrón, José M.

doi:10.1002/elan.201300269

Cited by 20 publications

(12 citation statements)

References 24 publications

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“…Compared with ordinary magnetic microspheres, magnetic composite microspheres exhibit good magnetic response and nano-synergistic effects in addition to the many characteristics of ordinary microspheres (such as a large surface area), and can also provide more functional groups such as –OH, –NH 2 , –COOH, and –CHO on their surfaces. Some studies have reported the use of magnetic composite nanomaterials in electrochemical immunosensors [ 149 ].…”

Section: Electrochemical Immunosensors Based On Various Nanomaterimentioning

confidence: 99%

Nanomaterials for Electrochemical Immunosensing

Pan

Yun

et al. 2017

Sensors

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Electrochemical immunosensors resulting from a combination of the traditional immunoassay approach with modern biosensors and electrochemical analysis constitute a current research hotspot. They exhibit both the high selectivity characteristics of immunoassays and the high sensitivity of electrochemical analysis, along with other merits such as small volume, convenience, low cost, simple preparation, and real-time on-line detection, and have been widely used in the fields of environmental monitoring, medical clinical trials and food analysis. Notably, the rapid development of nanotechnology and the wide application of nanomaterials have provided new opportunities for the development of high-performance electrochemical immunosensors. Various nanomaterials with different properties can effectively solve issues such as the immobilization of biological recognition molecules, enrichment and concentration of trace analytes, and signal detection and amplification to further enhance the stability and sensitivity of the electrochemical immunoassay procedure. This review introduces the working principles and development of electrochemical immunosensors based on different signals, along with new achievements and progress related to electrochemical immunosensors in various fields. The importance of various types of nanomaterials for improving the performance of electrochemical immunosensor is also reviewed to provide a theoretical basis and guidance for the further development and application of nanomaterials in electrochemical immunosensors.

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Section: Electrochemical Immunosensors Based On Various Nanomaterimentioning

confidence: 99%

Nanomaterials for Electrochemical Immunosensing

Pan

Yun

et al. 2017

Sensors

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“…Two different types of MMPs (ProtA- or Strep-functionalized) were analytically compared for the development of electrochemical immunosensors for ceruloplasmin (Cp), a relevant inflammation biomarker, using direct competitive formats with synthesized AP-Cp conjugate [25]. The determination of Cp was made by DPV measurement of 1-naphthol generated after addition of 1-naphthyl phosphate upon capturing the corresponding MMPs immunoconjugates onto SPCEs.…”

Section: Micromagnetic Particlesmentioning

confidence: 99%

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Yáñez‐Sedeño

Campuzano

Pingarrón

2016

Sensors

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Ultrasensitive biosensing is currently a growing demand that has led to the development of numerous strategies for signal amplification. In this context, the unique properties of magnetic particles; both of nano- and micro-size dimensions; have proved to be promising materials to be coupled with disposable electrodes for the design of cost-effective electrochemical affinity biosensing platforms. This review addresses, through discussion of selected examples, the way that nano- and micro-magnetic particles (MNPs and MMPs; respectively) have contributed significantly to the development of electrochemical affinity biosensors, including immuno-, DNA, aptamer and other affinity modes. Different aspects such as type of magnetic particles, assay formats, detection techniques, sensitivity, applicability and other relevant characteristics are discussed. Research opportunities and future development trends in this field are also considered.

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“…Ojeda et al [ 124 ] prepared and described the first electrochemical immunosensors for determination of Cp in 2013. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp Abs were compared using competitive immunoassay format with synthesized alkaline phosphatase (ALP)-Cp conjugate.…”

Section: Biomarkers As Analytesmentioning

confidence: 99%

Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers

2014

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Nanotechnology has played a crucial role in the development of biosensors over the past decade. The development, testing, optimization, and validation of new biosensors has become a highly interdisciplinary effort involving experts in chemistry, biology, physics, engineering, and medicine. The sensitivity, the specificity and the reproducibility of biosensors have improved tremendously as a result of incorporating nanomaterials in their design. In general, nanomaterials-based electrochemical immunosensors amplify the sensitivity by facilitating greater loading of the larger sensing surface with biorecognition molecules as well as improving the electrochemical properties of the transducer. The most common types of nanomaterials and their properties will be described. In addition, the utilization of nanomaterials in immunosensors for biomarker detection will be discussed since these biosensors have enormous potential for a myriad of clinical uses. Electrochemical immunosensors provide a specific and simple analytical alternative as evidenced by their brief analysis times, inexpensive instrumentation, lower assay cost as well as good portability and amenability to miniaturization. The role nanomaterials play in biosensors, their ability to improve detection capabilities in low concentration analytes yielding clinically useful data and their impact on other biosensor performance properties will be discussed. Finally, the most common types of electroanalytical detection methods will be briefly touched upon.

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Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin

Cited by 20 publications

References 24 publications

Nanomaterials for Electrochemical Immunosensing

Nanomaterials for Electrochemical Immunosensing

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers

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