Electrochemical Sensing for Naproxen Enantiomers Using Biofunctionalized Reduced Graphene Oxide Nanosheets

Abstract

A chiral platform was designed for enantioselective recognition of naproxen (Nap) enantiomers using BSA biofunctionalized nanosheets of Toluidin blue O (TBO) and reduced graphene oxide (rGO) on the glassy carbon electrode. The novel TBO@rGO nanosheets acting as a supporter for the chiral selector (BSA) and electrochemical signal indicator had plenty of advantages, which were presented and explored via ultraviolet-visible (UV-vis) spectroscopy, scanning electron microscope (SEM) and electrochemical method. Kinetics showed the chiral platform had good redox signal and fast electron transfer. Under optimal conditions, much larger signal discrepancy was obtained after the chiral interface interacted with R-Nap, and a linear electrochemical response to Nap enantiomers was obtained from 5.0 × 10 −4 mol L −1 to 5.0 × 10 −3 mol L −1 with a low detection limit of 3.3 × 10 −7 mol L −1 . Meanwhile, quartz crystal microbalance (QCM) for the interactions between BSA and Nap enantiomers further confirmed that R-Nap had a higher affinity with BSA. Furthermore, this simple chiral biosensor with excellent sensitivity and high stability is very expected to have more application in enantioselective recognition.

A new chiral electrochemical sensor for the enantioselective recognition of naproxen enantiomers using l-cysteine self-assembled over gold nanoparticles on a gold electrode

“…29 The proposed sensor was compared with the reported one. 29 The proposed sensor was compared with the reported one.…”

“…Huang et al fabricated a chiral sensor with a nanostructured composite for enantioselective recognition of lysine enantiomers. 29 Chen et al fabricated a chiral electrochemical sensor through chemical linking L-methotrexate onto the gold electrode surface for the enantioselective recognition of penicillamine enantiomers. 27 Nie et al constructed a chiral electrochemical sensor via cysteic acid modified glassy carbon electrode to discriminate tyrosine enantiomers.…”

Graphene-based hybrid for enantioselective sensing applications

“…Due to the fact that graphene surface can acquire various functionalities through the creation of different receptor units and bioactive scaffolds (Gravagnuolo et al, 2015), graphene-based enantioselective sensors have attracted widespread attention in electrochemical chiral sensing and discrimination platform for bioactive molecules such as naproxen (Guo et al, 2014), cystine (Zor et al, 2015).…”

A Voltammetric Sensory System for Recognition of Propranolol Enantiomers Based on Glassy Carbon Electrodes Modified by Polyarylenephthalide Composites of Melamine and Cyanuric Acid

“…Among EES, voltammetric enantioselective sensors are of particular interest , as they are more sensitive and universally applicable in comparison with potentiometric sensors. However, they are characterised by relatively low selectivity because the oxidation/reduction peaks on the voltammograms and the analytical signals of many enantiomers have slight differences.…”