Electrochemical studies of the effect of lanthanide ions on the activity of glutamate dehydrogenase

Zhuang, Qian-Kun; Dai, Huai-Cheng; Gao, Xiaoxia; Xin, Wenkuan

doi:10.1016/s0302-4598(00)00080-5

Cited by 10 publications

(10 citation statements)

References 17 publications

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“…Eu 3+ can slightly enhance the activity of the enzyme since the catalytic currents increase slowly with the concentration of Eu 3+ . This characteristic is in agreement with that obtained with GlDH in solution (Zhuang et al, 2000). Fig.…”

Section: Electrochemical Oxidation Of Glutamate At the Gldh-th-swnts/supporting

confidence: 92%

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Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode

Meng¹,

Wu²,

Chen³

et al. 2009

Biosensors and Bioelectronics

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A glutamate biosensor based on the electrocatalytic oxidation of reduced nicotinamide adenine dinucleotide (NADH), which was generated by the enzymatic reaction, was developed via employing a single-walled carbon nanotubes/thionine (Th-SWNTs) nanocomposite as a mediator and an enzyme immobilization matrix. The biosensor, which was fabricated by immobilizing glutamate dehydrogenase (GlDH) on the surface of Th-SWNTs, exhibited a rapid response (ca. 5s), a low detection limit (0.1 microM), a wide and useful linear range (0.5-400 microM), high sensitivity (137.3+/-15.7) microA mM(-1)cm(-2), higher biological affinity, as well as good stability and repeatability. In addition, the common interfering species, such as ascorbic acid, uric acid, and 4-acetamidophenol, did not cause any interference due to the use of a low operating potential (190 mV vs. NHE). The biosensor can be used to quantify the concentration of glutamate in the physiological level. The Th-SWNTs system represents a simple and effective approach to the integration of dehydrogenase and electrodes, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications including biosensors and biofuel cells.

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Section: Electrochemical Oxidation Of Glutamate At the Gldh-th-swnts/supporting

confidence: 92%

“…Zn 2+ can induce a conformation change and thus causes inhibition of the enzyme (Bell et al, 1987). Lanthanide ions La 3+ and Eu 3+ can enhance the activity of the enzyme (Zhuang et al, 2000). However, those results were obtained with GlDH in solution.…”

Section: Electrochemical Oxidation Of Glutamate At the Gldh-th-swnts/mentioning

confidence: 89%

Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode

Meng¹,

Wu²,

Chen³

et al. 2009

Biosensors and Bioelectronics

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“…Differential pulse polarography and cyclic voltammetry experiments were performed by using a BAS-100B Electrochemical Analyzer (USA) with a three-electrode system, which could be used to determine the enzyme activities [8,23,24]. A hanging mercury drop electrode (HMDE) was used as the working electrode.…”

Section: Methodsmentioning

confidence: 99%

“…For the cation effects, Ca 2+ ion [4] and Mg 2+ ion [5] have been reported to interact with the liver enzyme and produce effects on protein stability. Zn 2+ ion [6,7] was reported to induce a conformation change and cause inhibition of the enzyme, and La 3+ and Eu 3+ ions [8] could activate the enzyme GDH because lanthanide ions can enhance the dissociation of the NAD + -GDH complex by combining preferentially to NAD + .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Studies of the Inhibition and Activation Effects of Al (III) on the Activity of Bovine Liver Glutamate Dehydrogenase

Yang

2005

Sensors

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Abstract:Since the study of Al 3+ ion on the enzyme activity by using of electrochemical techniques was rarely found in available literatures, the differential-pulse polarography (DPP) technique was applied to study the effects of Al At the plant and animal physiologically relevant pH values (pH=6.5 and 7.5), the GDH enzyme activities were strongly depended on the concentrations of the metal ion in the assay mixture solutions. In the lower Al (III) concentration solutions (<30 µM), the inhibitory effects were shown, which are in accordance with the recently biological findings. With the increase of Al (III) concentrations (30~80 µM), the enzyme GDH activities were activated. However, once the concentration of Al (III) arrived to near 0.1 mM level (>80µM), the inhibition effects of Al (III) were shown again. The cyclic voltammetry of NAD + and NAD + -GDH in the presence of Al (III) can help to explain some biological phenomena. According to the differential-pulse polarography and cyclic voltammetry experiments, the present research confirmed that the electrochemical technique is a convenient and reliable sensor for accurate determination of enzyme activity in biological and environmental samples.

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“…) ions can enhance the activity of bovine GLDH, at least in solution [80]. Also, Meng et al observed a decrease in catalytic currents generated by GLDH when Zn 2+ was added to the solution with the inhibitory effect increasing with increasing concentrations of the zinc ions [67].…”

Section: +mentioning

confidence: 99%

Enzyme-Based Electrochemical Glutamate Biosensors

Okon¹,

Ronkainen²

2017

Electrochemical Sensors Technology

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Glutamate, a major excitatory neurotransmitter in the mammalian central nervous system, plays a vital role in many physiological processes and is one of the key neurotransmitters of interest in psychopharmacology. It is involved in many normal and abnormal behaviors related to neurological and psychiatric disorders. The glutamate system has been proposed to play a significant role in various neurological and psychiatric disorders such as Alzheimer's disease, autism, schizophrenia, depression, drug addiction, and more. The design, construction, and optimization of enzyme-based electrochemical biosensors for in vivo and in vitro detection of glutamate are active areas of interdisciplinary research. For example, various glutamate biosensors have been developed for monitoring dynamic levels of extracellular glutamate in the living brain tissue adding to the current medical knowledge of these complex neurotransmitter systems and ultimately impacting treatment plans. In addition to biological sciences and clinical medicine, glutamate biosensors have been used in environmental monitoring, in the fermentation industry, and in the food industry for determination of monosodium glutamate (MSG), a common flavor-enhancing food additive.

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Electrochemical studies of the effect of lanthanide ions on the activity of glutamate dehydrogenase

Cited by 10 publications

References 17 publications

Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode

Low potential detection of glutamate based on the electrocatalytic oxidation of NADH at thionine/single-walled carbon nanotubes composite modified electrode

Electrochemical Studies of the Inhibition and Activation Effects of Al (III) on the Activity of Bovine Liver Glutamate Dehydrogenase

Enzyme-Based Electrochemical Glutamate Biosensors

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