Electrochemiluminescence Biosensor Based on Conducting Poly(5-formylindole) for Sensitive Detection of Ramos Cells

Abstract

A signal-on electrochemiluminescence (ECL) biosensor devoted to the detection of Ramos cells was fabricated based on a novel conducting polymer, poly(5-formylindole) (P5FIn), which was synthesized electrochemically by direct anodic oxidation of 5-formylindole (5FIn). This ECL platform was presented by covalently coupling the 18-mer amino-substituted oligonucleotide (ODN) probes with aldehyde groups that are strongly reactive toward a variety of nucleophiles on the surface of solid substrates. The specific identification and high-affinity between aptamers and target cells, gold nanoparticles (AuNPs) enhanced ECL nanoprobes, along with P5FIn induced ECL quenching contributed greatly to the sensitivity and selectivity. The ECL signals were logarithmically linear with the concentration of Ramos cells in a wide determination range from 500 to 1.0 × 10(5) cells mL(-1), and the corresponding detection limit was 300 cells mL(-1).

Effect of substituent position on electrodeposition, morphology, and capacitance performance of polyindole bearing a carboxylic group

“…Currently, there are many indole derivatives containing functional groups such as -NO 2 [25,26], -CN [27,28], -COOH [29], -Br [30], -Cl [31], -F [31], -CH 3 [32,33], -OCH 3 [34], -C 6 H 5 [35], -CHO [36] and -NH 2 [37,38], which can be polymerized into the polyindoles family. Among polyindole derivatives, the carboxylic substituent on polyindole benefits the formation of nanowires possibly due to the hydrogen bond interactions [39], and may lead to the higher capacitance and the better cycling stability relative to unmodified polyindole [40,41].…”

Controlled synthesis of partially reduced graphene oxide: Enhance electrochemical determination of isoniazid with high sensitivity and stability

“…The reduction degree of ERGO was controlled by performing certain cycle number of CVs. For comparison, ERGO films with different reduction cycle numbers (1,3,5,7,9,11,13,15) were prepared and marked as ERGO 1 , ERGO 3 , ERGO 5 , ERGO 7 , ERGO 9 , ERGO 11 , ERGO 13 and ERGO 15 , respectively.…”

“…However, these methods suffer from disadvantages, such as expensive facilities, complex sample pretreatment and time-consuming. Electrochemical methods have been proven to be simple, fast, sensitive, and reliable for the determination of numerous electroactive drug components in pharmaceutical dosage forms and biological fluids [8][9][10][11]. However, many reported sensors for the electrochemical detection of INZ usually exist problems associated with electrode surface fouling caused by reaction products, which greatly influences their repeatability and sensitivity [12,13].…”

An “Off–On” Electrochemiluminescent Biosensor Based on DNAzyme-Assisted Target Recycling and Rolling Circle Amplifications for Ultrasensitive Detection of microRNA

“…A typical Pb 2+ -requiring DNAzyme design named "8-17" is consisted of a substrate strand (17DS) and an enzyme strand (17E) [21][22] 31,32 . In addition, the quenching of luminol ECL signal was also studied [33][34][35][36] .…”