Electrochemiluminescence imaging of latent fingermarks through the immunodetection of secretions in human perspiration

Xu, Linru; Zhou, Zhenyu; Zhang, Congzhe; He, Yayun; Su, Bin

doi:10.1039/c4cc03466j

Cited by 73 publications

(49 citation statements)

References 40 publications

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“…Figure 2(a) illustrates the general principle of the enzyme immunoassay and the ECL imaging for the detection of secretions in LFPs [71]. First, the primary antibodies specific for target analytes were incubated with the fingerprint sample.…”

Section: Immunological Techniquesmentioning

confidence: 99%

Advances in the development and component recognition of latent fingerprints

Zhang

et al. 2015

Sci. China Chem.

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Section: Immunological Techniquesmentioning

confidence: 99%

Advances in the development and component recognition of latent fingerprints

Zhang

et al. 2015

Sci. China Chem.

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“…Our group has demonstrated that LFPs can be visualized by ECL through the preceding recognition of specific analytes with enzyme-linked immune-recognition methodology [70]. Three proteins/polypeptides presented in eccrine fingermarks, epidermal growth factor (EGF), lysozyme, and dermcidin, have been successfully detected specifically, with the fingermarks visualized simultaneously.…”

Section: Imaging Reactivity At Electrode Surfacesmentioning

confidence: 99%

Section: Imaging Analysis At the Single Microbead Levelmentioning

confidence: 99%

Imaging Analysis Based on Electrogenerated Chemiluminescence

et al. 2017

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Electrochemiluminescence (ECL), also called electrogenerated chemiluminescence, is luminescence that is produced by chemical reactions triggered by electrochemical method. ECL imaging is a novel imaging technique, which can simultaneously provide two kinds of signals of both electrochemistry and optical image. Over the past two decades, ECL imaging has been not only a powerful tool for investigating the fundamental scientific questions such as ECL mechanisms and reaction kinetics, but also a versatile analytical technique for detection of a wide range of analytes including small molecules, DNA, proteins, and cells. In the first part of this review, we briefly describe the reaction mechanisms of ECL generation. Then the review focuses on the research progress on the ECL imaging approach. It is basically introduced from the following five aspects: (i) visualization of electroactive sites on surfaces, (ii) imaging analysis at the single-bead and single-cell levels, (iii) array bioanalysis, (iv) multi-color ECL imaging, and (v) paper chip based on ECL imaging. Finally, some perspectives and future directions in this active research area are presented.

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“…Wood et al reported a highly selective technique using a lysozyme targeting-aptamer-based reagent to visualize latent fingermarks with fluorescence images [14]. Shan et al [15] and Xu et al [16] respectively combined electrochemistry with surface plasmon resonance (SPR) and enzyme immunoassay to detect fingermarks. Li et al used the SPR of aptamer-tagged Au nanoparticles (NPs) to visualize fingermarks [17, 18].…”

Section: Introductionmentioning

confidence: 99%

Rapid visualization of latent fingermarks using gold seed-mediated enhancement

Cheng

2016

J Nanobiotechnol

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BackgroundFingermarks are one of the most important and useful forms of physical evidence in forensic investigations. However, latent fingermarks are not directly visible, but can be visualized due to the presence of other residues (such as inorganic salts, proteins, polypeptides, enzymes and human metabolites) which can be detected or recognized through various strategies. Convenient and rapid techniques are still needed to provide obvious contrast between the background and the fingermark ridges and to then visualize latent fingermark with a high degree of selectivity and sensitivity.ResultsIn this work, lysozyme-binding aptamer-conjugated Au nanoparticles (NPs) are used to recognize and target lysozyme in the fingermark ridges, and Au+-complex solution is used as a growth agent to reduce Au+ from Au+ to Au0 on the surface of the Au NPs. Distinct fingermark patterns were visualized on a range of professional forensic within 3 min; the resulting images could be observed by the naked eye without background interference. The entire processes from fingermark collection to visualization only entails two steps and can be completed in less than 10 min. The proposed method provides cost and time savings over current fingermark visualization methods.ConclusionsWe report a simple, inexpensive, and fast method for the rapid visualization of latent fingermarks on the non-porous substrates using Au seed-mediated enhancement. Au seed-mediated enhancement is used to achieve the rapid visualization of latent fingermarks on non-porous substrates by the naked eye without the use of expensive or sophisticated instruments. The proposed approach offers faster detection and visualization of latent fingermarks than existing methods. The proposed method is expected to increase detection efficiency for latent fingermarks and reduce time requirements and costs for forensic investigations.

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Electrochemiluminescence imaging of latent fingermarks through the immunodetection of secretions in human perspiration

Cited by 73 publications

References 40 publications

Advances in the development and component recognition of latent fingerprints

Advances in the development and component recognition of latent fingerprints

Imaging Analysis Based on Electrogenerated Chemiluminescence

Rapid visualization of latent fingermarks using gold seed-mediated enhancement

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