Electrochromatography in poly(dimethyl)siloxane microchips using organic monolithic stationary phases

Faure, Karine; Blas, Maximilien; Yassine, Omar; Delaunay, Nathalie; Crétier, G.; Albert, Monique; Rocca, Jean‐Louis

doi:10.1002/elps.200600566

Cited by 46 publications

(38 citation statements)

References 28 publications

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“…[48][49][50] A mold was fabricated using a 7.5 lm thick layer of AZ 9260 that was spin coated on a silicon wafer, exposed to UV light and developed. Liquid PDMS was spread over the entire surface of the mold, cured at 75 C for 2 h and then gently peeled off.…”

Section: -5mentioning

confidence: 99%

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

et al. 2014

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This study describes the development and testing of a magnetic microfluidic chip (MMC) for trapping and isolating cells tagged with superparamagnetic beads (SPBs) in a microfluidic environment for selective treatment and analysis. The trapping and isolation are done in two separate steps; first, the trapping of the tagged cells in a main channel is achieved by soft ferromagnetic disks and second, the transportation of the cells into side chambers for isolation is executed by tapered conductive paths made of Gold (Au). Numerical simulations were performed to analyze the magnetic flux and force distributions of the disks and conducting paths, for trapping and transporting SPBs. The MMC was fabricated using standard microfabrication processes. Experiments were performed with E. coli (K12 strand) tagged with 2.8 μm SPBs. The results showed that E. coli can be separated from a sample solution by trapping them at the disk sites, and then isolated into chambers by transporting them along the tapered conducting paths. Once the E. coli was trapped inside the side chambers, two selective treatments were performed. In one chamber, a solution with minimal nutrition content was added and, in another chamber, a solution with essential nutrition was added. The results showed that the growth of bacteria cultured in the second chamber containing nutrient was significantly higher, demonstrating that the E. coli was not affected by the magnetically driven transportation and the feasibility of performing different treatments on selectively isolated cells on a single microfluidic platform.

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Section: -5mentioning

confidence: 99%

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

et al. 2014

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“…The most widely used method for the synthesis of these monolithic columns is a free-radical polymerization of a mixture consisting of one or more functional monomers, including a crosslinker, a porogenic solvent and an initiator. Most of the literature concerning acrylate-based monoliths is focused on the use of butyl acrylate (BA) [4][5][6][7][8][9][10][11] and hexyl acrylate [11][12][13][14][15] as bulk monomers. However, only few studies related to the employ of other longer alkyl chain monomers such as lauryl acrylate (LA) [10,16] or stearyl acrylate [17,18] can be found.…”

Section: Introductionmentioning

confidence: 99%

Chemical initiation for butyl and lauryl acrylate monolithic columns for CEC

et al. 2009

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Butyl acrylate (BA)- and lauryl acrylate (LA)-based monolithic stationary phases for CEC were synthesized, using a redox system as initiator of polymerization. BA monoliths were initiated with ammonium peroxodisulfate, whereas LA columns were obtained with lauroyl peroxide as initiator. In both cases, TEMED was used to activate the process. The influence of porogenic solvent composition on both morphological and electrochromatographic properties of the resulting monoliths was investigated. Excellent efficiencies (minimum plate heights of 4.2-6.3 microm for BA columns and 2.6-5.3 microm for LA stationary phases, for a PAHs mixture) were achieved. The capability of separation of both types of monolithic beds was compared by the analysis of complex mixtures of polycyclic aromatic hydrocarbons and anabolic steroids.

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“…In this case, crosslinker allyl-g-or allyl-b-CD played as a chiral selector for enantioseparation of FITC-labeled dansylated amino acids. Covalent coating with a first layer of acrylate polymer was found to limit adsorption and subsequent photopolymerization of organic acrylate monolith was attainable [50].…”

Section: Monoliths and Continuous Structuresmentioning

confidence: 99%

Liquid chromatography on chip

Faure

2010

Electrophoresis

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LC is one of the most powerful separation techniques as illustrated by its leading role in analytical sciences through both academic and industrial communities. Its implementation in microsystems appears to be crucial in the development of mu-Total Analysis System. If electrophoretic techniques have been widely used in miniaturized devices, LC has faced multiple challenges in the downsizing process. During the past 5 years, significant breakthroughs have been achieved in this research area, in both conception and use of LC on chip. This review emphasizes the development of novel stationary phases and their implementation in microchannels. Recent instrumental advances are also presented, highlighting the various driving forces (pressure, electrical field) that have been selected and their respective ranges of applications.

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Electrochromatography in poly(dimethyl)siloxane microchips using organic monolithic stationary phases

Cited by 46 publications

References 28 publications

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

Chemical initiation for butyl and lauryl acrylate monolithic columns for CEC

Liquid chromatography on chip

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