Electroenzymatic degradation of azo dye using an immobilized peroxidase enzyme

Potential applications of peroxidases

“…Most applications have focussed on the treatment of phenolic contaminants in the presence of H 2 O 2 (Klibanov, Alberti, Morris, & Felshin, 1980;Klibanov, Tu, & Scott 1982;Klibanov, 1983;Buchnan & Nicell, 1997;Varon et al, 1998;Mantha et al, 2002;Filizola & Loew, 2000;Kim, Lee, Cho, Shim, & Moon, 2005;Mohan, Prasad, Rao, & Sarma, 2005;Cheng, Yu, & Zuo, 2006). The use of HRP for the treatment of contaminants including anilines, hydroxyquinoline and arylamine carcinogens, such as benzidines and naphthylamines, has also been demonstrated in the laboratory (Klibanov, Alberti, Morris, & Felshin, 1980;Klibanov & Morris, 1981).…”

Evaluation of hematin-catalyzed Orange II degradation as a potential alternative to horseradish peroxidase

“…As the reaction proceeded further, substituted aromatic amines were generated and shifts to their characteristic wavelengths were observed. Kim et al (2005) postulated that sulfanilic acid was formed, on the basis of the peak at 247 nm, which may be assigned to sulfanilates. It is interesting that with both catalysts this peak is clear in the products.…”

Peroxidase mediated decolorization and remediation of wastewater containing industrial dyes: a review

“…An electroenzymatic process is an interesting approach that combines enzyme catalysis and electrode reactions. Kim et al (2005) studied an electroenzymatic method that uses an immobilized HRP to degrade Orange II, an azo dye within a twocompartment packed-bed flow reactor. Electroenzymatic degradation of Orange II was performed by …”