Electrokinetically Controlled Microfluidic Analysis Systems

Bousse, Luc; Cohen, Claudia B.; Nikiforov, Theo T.; Chow, Andrea W.; Kopf-Sill, Anne; Dubrow, Robert; Parce, J W

doi:10.1146/annurev.biophys.29.1.155

Cited by 200 publications

(145 citation statements)

References 88 publications

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“…If I f is known, u can be calculated using eqn. (3). The signal from the optical detector increases with the total photoemission from the measuring volume.…”

Section: Theoretical Backgroundmentioning

confidence: 98%

“…(3) indicates that for a given system (dye, buffer, and laser beam property), I f increases with u. If I f is known, u can be calculated using eqn.…”

Section: Theoretical Backgroundmentioning

confidence: 99%

“…3,4 The flow is not only driven by pressure difference, but also by electrokinetic force, i.e. electroosmotics and electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

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Laser induced fluorescence photobleaching anemometer for microfluidic devices

Wang

2005

Lab Chip

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We have developed a novel, non-intrusive fluid velocity measurement method based on photobleaching of a fluorescent dye for microfluidic devices. The residence time of the fluorescent dye in a laser beam depends on the flow velocity and approximately corresponds to the decaying time of the photobleaching of the dye in the laser beam. The residence time is inversely proportional to the flow velocity. The fluorescence intensity increases with the flow velocity due to the decrease of the residence time. A calibration curve between fluorescence intensity and known flow velocity should be obtained first. The calibration relationship is then used to calculate the flow velocity directly from the measured fluorescence intensity signal. The new method can measure the velocity very quickly and is easy to use. It is demonstrated for both pressure driven flow and electroosmotic flow.

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“…If I f is known, u can be calculated using eqn. (3). The signal from the optical detector increases with the total photoemission from the measuring volume.…”

Section: Theoretical Backgroundmentioning

confidence: 98%

“…(3) indicates that for a given system (dye, buffer, and laser beam property), I f increases with u. If I f is known, u can be calculated using eqn.…”

Section: Theoretical Backgroundmentioning

confidence: 99%

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Laser induced fluorescence photobleaching anemometer for microfluidic devices

Wang

2005

Lab Chip

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“…This is the main reason why they have attracted so much interest in recent years [1]. For pumping, electroosmotic flow (EOF) is usually the method of choice as it is rather easy to implement experimentally compared to pressure-driven flow [2][3][4][5]. However, the main weakness of EOF is the stability of the flow rate, and perhaps this is why capillary electrophoresis (CE) as a separation technique is not always as reproducible as required.…”

Section: Introductionmentioning

confidence: 99%

Polyelectrolyte‐modified short microchannel for cation separation

2004

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Three alkali cations, potassium, sodium, and lithium, have been separated within 15 s in a 1 cm long polymer microchip. The separation microchannel is modified by a polycation, poly(allylammonium chloride), which makes the channel surfaces positively charged leading to a reversed electroosmotic flow (EOF) when compared to bare channels. Due to the decreased apparent mobility of the cations, the separation resolution is improved allowing the use of shorter channels.

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“…Bousse et al 18 suggested that analyzing one sample within 1 min, and a set of ten samples on one LabChip takes approximately 25-30 min. 18 The peak number, molecular weight ͑present as kDa͒, migration time ͑present as second͒, and protein content of these proteins are shown in Figs. 3 and …”

Section: B Comparison Of Glycoproteins By Labchip and Sds-pagementioning

confidence: 99%

Comparative studies on the analysis of glycoproteins and lipopolysaccharides by the gel-based microchip and SDS-PAGE

Hsieh¹,

Chen²

2007

Biomicrofluidics

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In order to determine time efficiency between the gel-based microchip ͑LabChip͒ and traditional sodium dodecyl sulfate-polyacrylamide gel electrophoresis ͑SDS-PAGE͒, glycoproteins and lipopolysaccharides were analyzed in this study. After 90 min of gel electrophoresis, glycoproteins ͑bovine serum albumin, lysozyme, ovalbumin, and apo-transferrin͒ and fluorescent lipopolysaccharides ͑LPS-O and LPS-S͒ under reducing conditions could be analyzed by SDS-PAGE, and it would take ͑including imaging and analyzing͒ more than 3 h. The same sample could also be assayed on a Bioanalyzer in combination with the LabChip, and it would only need 30 min from start to finish. The assay software automatically calculated the size and concentration of each separated peak and displayed the results in real time, thus eliminating time-consuming procedures such as imaging and analyzing. Compared to the traditional reducing SDS-PAGE, LabChip has a faster turnaround time.

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Electrokinetically Controlled Microfluidic Analysis Systems

Cited by 200 publications

References 88 publications

Laser induced fluorescence photobleaching anemometer for microfluidic devices

Laser induced fluorescence photobleaching anemometer for microfluidic devices

Polyelectrolyte‐modified short microchannel for cation separation

Comparative studies on the analysis of glycoproteins and lipopolysaccharides by the gel-based microchip and SDS-PAGE

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