Electrolytically generated acid functional water inhibits NF-<b>κ</b>B activity by attenuating nuclear-cytoplasmic shuttling of p65 and p50 subunits

Gojoubori, Takahiro; Ota, Hirotaka; Kusunoki, Masafumi; Nishio, Yukina; Nishio, Kensuke; Iwasa, Satoko; Kaneko, Y; Asano, Masaharu

doi:10.3109/10799893.2015.1086883

Cited by 4 publications

(4 citation statements)

References 21 publications

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“…Such retrotransport of NF-κB is also dependent of the dynein/dynactin motor complex [116]. Like SRs, NF-κB dimers are also subject of a dynamic nuclear-cytoplasmic shuttling [117,118]. Therefore, the IκB/NF-κB complex also undergoes dynamic dissociation and reassociation events.…”

Section: Immunophilins Regulate Nf-κb Activitymentioning

confidence: 99%

Biological Actions of the Hsp90-binding Immunophilins FKBP51 and FKBP52

et al. 2019

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Immunophilins are a family of proteins whose signature domain is the peptidylprolyl-isomerase domain. High molecular weight immunophilins are characterized by the additional presence of tetratricopeptide-repeats (TPR) through which they bind to the 90-kDa heat-shock protein (Hsp90), and via this chaperone, immunophilins contribute to the regulation of the biological functions of several client-proteins. Among these Hsp90-binding immunophilins, there are two highly homologous members named FKBP51 and FKBP52 (FK506-binding protein of 51-kDa and 52-kDa, respectively) that were first characterized as components of the Hsp90-based heterocomplex associated to steroid receptors. Afterwards, they emerged as likely contributors to a variety of other hormone-dependent diseases, stress-related pathologies, psychiatric disorders, cancer, and other syndromes characterized by misfolded proteins. The differential biological actions of these immunophilins have been assigned to the structurally similar, but functionally divergent enzymatic domain. Nonetheless, they also require the complementary input of the TPR domain, most likely due to their dependence with the association to Hsp90 as a functional unit. FKBP51 and FKBP52 regulate a variety of biological processes such as steroid receptor action, transcriptional activity, protein conformation, protein trafficking, cell differentiation, apoptosis, cancer progression, telomerase activity, cytoskeleton architecture, etc. In this article we discuss the biology of these events and some mechanistic aspects.

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Section: Immunophilins Regulate Nf-κb Activitymentioning

confidence: 99%

Biological Actions of the Hsp90-binding Immunophilins FKBP51 and FKBP52

et al. 2019

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“…38,39) As a plausible mechanism involving acidic water, acidic water has been known to induce hBD2, irrespective of NF-κB. 13,14) Strategically, acidic water inhibits NF-κB activity by attenuating nuclear-cytoplasmic shuttling of p65 and p50 subunits. 14) Further, increased NF-κB expression would induce cutaneous inflammation, 40) consequently hBD might improve wound healing.…”

Section: Discussionmentioning

confidence: 99%

“…13,14) Strategically, acidic water inhibits NF-κB activity by attenuating nuclear-cytoplasmic shuttling of p65 and p50 subunits. 14) Further, increased NF-κB expression would induce cutaneous inflammation, 40) consequently hBD might improve wound healing. 41) These potential mechanisms implicated in wound healing remain to be elucidated in our on-going acidic water study.…”

Section: Discussionmentioning

confidence: 99%

“…Current in vitro studies hinted that functional acidic water might be correlated to epithelial signalling pathway regarding hBD2 and nuclear factor-kappa B (NF-κB) regulation on human oral squamous cell carcinoma. 13,14) This signal path might be plausible to elucidate the molecular mechanism of acidic water on wound healing. Up to date, three studies have been involved in using electrolyzed water in wound healing.…”

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Wound Healing Effect of Slightly Acidic Electrolyzed Water on Cutaneous Wounds in Hairless Mice <i>via</i> Immune-Redox Modulation

You

Fadriquela

Sajo

et al. 2017

Biological & Pharmaceutical Bulletin

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Acidic electrolyzed water is an innovative sanitizer having a wide-spectrum of applications in food industry, and healthcare industry but little is known on its effect and mechanism in wound healing. The study was conducted to identify the effect and mechanism of slightly acidic electrolyzed water (SAEW) on cutaneous wounds in hairless mice. SAEW (pH: 5-6.5, oxidation reduction potential: 800 mV, chlorine concentration: 25 ppm) was prepared through electrolysis of water and was applied to the wounds of hairless mice three times a day for seven days. Wound size, immune response and oxidative stress were explored and compared to conventional agents such as Betadine and alcohol. We found that SAEW-treated group showed the highest wound reduction percentage ( p<0.01). Antioxidant activities such as glutathione peroxidase, catalase and myeloperoxidase activities of SAEW group surpassed the total reactive oxygen species in skin. Nuclear factor erythroid-2-related-factor-2 and aryl hydrocarbon receptor were upregulated in SAEW group. Further, SAEW recruited the production of intracellular calcium and promoted its utilization for faster healing. In line, SAEW treatment decreased pro-inflammatory cytokines [interleukin (IL)-1β, IL-6, keratinocyte chemoattractant, and tumor necrosis factor-α] in serum. Other hallmarks of wound healing, matrixmetalloproteinases (MMP)1 and MMP9 were also upregulated. Collectively, our study indicates that SAEW is effective in wound healing of hairless mice via immune-redox modulation, and heals better/faster than conventional agents.Key words slightly acidic electrolyzed water; wound healing; oxidative stress; immune response Skin injuries, such as cutaneous wounds, need to undergo a complex mechanism to repair the damage. Wound healing is comprised of four sequential but overlapping stages; homeostasis, inflammation, proliferation, and remodeling, strongly regulated with the goal of restoring the integrity of the skin.

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Germanium Reduces Inflammatory Damage in Mammary Glands During Lipopolysaccharide-Induced Mastitis in Mice

Wang

Teng

Zhou

et al. 2020

Biol Trace Elem Res

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Electrolytically generated acid functional water inhibits NF-κB activity by attenuating nuclear-cytoplasmic shuttling of p65 and p50 subunits

Abstract: The results from this study indicate that FW might inhibit spontaneous IL-8 secretion by redistribution of the NF-κB subunits within the cells.

Cited by 4 publications

References 21 publications

Biological Actions of the Hsp90-binding Immunophilins FKBP51 and FKBP52

Biological Actions of the Hsp90-binding Immunophilins FKBP51 and FKBP52

Wound Healing Effect of Slightly Acidic Electrolyzed Water on Cutaneous Wounds in Hairless Mice <i>via</i> Immune-Redox Modulation

Germanium Reduces Inflammatory Damage in Mammary Glands During Lipopolysaccharide-Induced Mastitis in Mice

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