Electromanipulation of mammalian cells: fundamentals and application

Zimmermann, U.; Friedrich, Ursula; Mussauer, H.; Geßner, P.; Hamel, Kristina; Sukhorukov, V.L.

doi:10.1109/27.842868

Cited by 120 publications

(77 citation statements)

References 24 publications

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“…Figure 3(b) shows an impedance plot for a 5 µm cell plotted from equation (29). This plot shows that the low frequency response is dominated by the double layer and that increasing the capacitance of the double layer diminishes this effect.…”

Section: Modelling and Simulationmentioning

confidence: 99%

“…The current passing through the system is measured as a function of frequency to give the electrical properties of the particles in suspension. Currently, there are numerous approaches for the dielectric measurement of biological suspensions and several excellent reviews of the techniques are available in the literature [26][27][28][29][30][31][32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

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Single cell dielectric spectroscopy

Morgan

Sun²,

Holmes³

et al. 2006

J. Phys. D: Appl. Phys.

395

313

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Over the last century a number of techniques have been developed which allow the measurement of the dielectric properties of biological particles in fluid suspension. The majority of these techniques are limited by the fact that they only provide an average value for the dielectric properties of a collection of particles. More recently, with the advent of microfabrication techniques and the Lab-on-a-chip, it has been possible to perform dielectric spectroscopic experiments on single biological particles suspended in physiological media. In this paper we review current methods for single cell dielectric spectroscopy. We also discuss alternative single cell dielectric measurement techniques, specifically the ac electrokinetic methods of dielectrophoresis and electrorotation. Single cell electrical impedance spectroscopy is also discussed with relevance to a microfabricated flow cytometer. We compare impedance spectroscopy data obtained from measurements made using a microfabricated flow cytometer with simulation data obtained using an equivalent circuit model for the device.

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Section: Modelling and Simulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Single cell dielectric spectroscopy

Morgan

Sun²,

Holmes³

et al. 2006

J. Phys. D: Appl. Phys.

395

313

View full text Add to dashboard Cite

show abstract

“…9 For many biomedical applications, this dielectric difference in combination with nonuniform electric fields can be used to translate cells ͑dielectrophoresis͒, or with uniform time-varying fields to rotate cells ͑electrorotation͒. 10 Recently, dielectrophoretic forces have been enhanced with improved control using multi-insulating blocks for manipulating polystyrene microspheres.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells

et al. 2008

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The mechanical behavior of cells offers insight into many aspects of their properties. We propose an approach to the mechanical analysis of cells that uses a combination of electromanipulation for stimulus and capacitance for sensing. To demonstrate this approach, polystyrene spheres and yeast cells flowing in a 25 m ϫ 100 m microfluidic channel were detected by a perpendicular pair of gold thin film electrodes in the channel, spaced 25 m apart. The presence of cells was detected by capacitance changes between the gold electrodes. The capacitance sensor was a resonant coaxial radio frequency cavity ͑2.3 GHz͒ coupled to the electrodes. The presence of yeast cells ͑Saccharomyces cerevisiae͒ and polystyrene spheres resulted in capacitance changes of approximately 10 and 100 attoFarad ͑aF͒, respectively, with an achieved capacitance resolution of less than 2 aF in a 30 Hz bandwidth. The resolution is better than previously reported in the literature, and the capacitance changes are in agreement with values estimated by finite element simulations. Yeast cells were trapped using dielectrophoretic forces by applying a 3 V signal at 1 MHz between the electrodes. After trapping, the cells were displaced using amplitude and frequency modulated voltages to produce modulated dielectrophoretic forces. Repetitive displacement and relaxation of these cells was observed using both capacitance and video microscopy.

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“…52,53 The main concern of the -EIS technique is that differences in impedance profiles between measurements taken with and without a cell's presence are usually small and sometimes unobservable. 51 In the meantime, several microfluidic-based devices have also been developed to measure the mechanical properties of single cells, such as micropipette aspiration, 54,55 electrodeformation, [56][57][58] and optical stretchers. 16,20,59,60 In micropipette aspiration, a cell is deformed by applying negative pressure through an aspiration channel.…”

Section: Introductionmentioning

confidence: 99%

A microfluidic device for simultaneous electrical and mechanical measurements on single cells

et al. 2011

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This paper presents a microfluidic device for simultaneous mechanical and electrical characterization of single cells. The device performs two types of cellular characterization ͑impedance spectroscopy and micropipette aspiration͒ on a single chip to enable cell electrical and mechanical characterization. To investigate the performance of the device design, electrical and mechanical properties of MC-3T3 osteoblast cells were measured. Based on electrical models, membrane capacitance of MC-3T3 cells was determined to be 3.

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Electromanipulation of mammalian cells: fundamentals and application

Cited by 120 publications

References 24 publications

Single cell dielectric spectroscopy

Single cell dielectric spectroscopy

Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells

A microfluidic device for simultaneous electrical and mechanical measurements on single cells

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