Electromechanical effects on multilayered cells in nonuniform rotating fields

Sebastián, J.L.; Muñoz, S.; Martı́nez, G; Álvarez, Gabriel

doi:10.1103/physreve.84.011926

Cited by 13 publications

(10 citation statements)

References 48 publications

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“…The authors have already performed a detailed study on the polarizability and electromechanical effects on multilayered cells in nonuniform rotating fields [35] where we showed that the effective polarizability of a particle consisting of N layers and immersed in a suspending medium of complex permittivityε ext = ε ext − iσ ext /ω can be determined by the equatioñ…”

Section: Polarizability and Rotational Velocity Of The Yeast Cellmentioning

confidence: 99%

“…For a detailed analysis of the influence of all the higher order multipolar terms induced in the cell by the nonuniform fields we refer the reader to Ref. [35]. The electrorotational torque is proportional to the out-of-phase part of the induced dipole moment and to the timeaveraged applied electric field [37,38].…”

Section: Polarizability and Rotational Velocity Of The Yeast Cellmentioning

confidence: 99%

“…For a spherical particle of radius R with complex permittivityε eq and polarizabilityα eq the torque is given by [35,40] …”

Section: Polarizability and Rotational Velocity Of The Yeast Cellmentioning

confidence: 99%

See 2 more Smart Citations

Dielectric Characterization of the Yeast Cell Budding Cycle

Franco¹,

Otero²,

Madronero³

et al. 2013

PIER

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Abstract-We combine experimental electrorotation data and the numerical analysis of the electrorotation chamber and cell to electrically characterize the Saccharomyces cerevisiae yeast budding cell cycle and to obtain the electrical parameters of the cell. To model the yeast cell we use spherical and doublet-shaped geometries with a four layered structure: cytoplasm, membrane, inner and outer walls. To derive the geometrical and electrical parameters of the yeast model we use the finite element method to calculate the yeast rotational velocity spectrum and apply the least-square method to fit the calculated values to experimental data. We show that the calculated yeast electrorotation spectra undergo significant changes throughout its budding cycle and that the calculated spectra fit experimental data obtained for 0% (start) and 50% representative budding stages very well. The analysis also shows the small variation of the rotation crossover frequency within a full span of the yeast growth cycle. As an application of this work, we apply the Maxwell-Wagner formalism to obtain the dielectric spectra of truly synchronized yeast suspensions.

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Section: Polarizability and Rotational Velocity Of The Yeast Cellmentioning

confidence: 99%

Section: Polarizability and Rotational Velocity Of The Yeast Cellmentioning

confidence: 99%

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Dielectric Characterization of the Yeast Cell Budding Cycle

Franco¹,

Otero²,

Madronero³

et al. 2013

PIER

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show abstract

“…DEP methods utilize electric fields for cell rotation, which may cause irreversible damage to the target cell. 26 Finally, acoustic and microfluid methods have difficulty stabilizing 6 and accessing 19 the target cell after cell orientation.…”

Section: ■ Introductionmentioning

confidence: 99%

Noncontact Manipulation of Intracellular Structure Based on Focused Surface Acoustic Waves

et al. 2023

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Cell orientation is essential in many applications in biology, medicine, and chemistry, such as cellular injection, intracellular biopsy, and genetic screening. However, the manual cell orientation technique is a trial-and-error approach, which suffers from low efficiency and low accuracy. Although several techniques have improved these issues to a certain extent, they still face problems deforming or disrupting cell membranes, physical damage to the intracellular structure, and limited particle size. This study proposes a noncontact and noninvasive cell orientation method that rotates a cell using surface acoustic waves (SAWs). To realize the acoustic cell orientation process, we have fabricated a microdevice consisting of two pairs of focused interdigital transducers (FIDTs). Instead of rotating the entire cell, the proposed method rotates the intracellular structure, the cytoplasm, directly through the cell membrane by acoustic force. We have tested the rotational manipulation process on 30 zebrafish embryos. The system was able to orientate a cell to a target orientation with a one-time success rate of 93%. Furthermore, the postoperation survival rate was 100%. Our acoustic rotational manipulation technique is noninvasive and easy to use, which provides a starting point for cell-manipulation-essential tasks, such as single-cell analysis, organism studies, and drug discovery.

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“…However, the magnetic fields' alignment restricted the achievable cell orientations. Dielectophoresis (DEP) was introduced to rotate cells using a non-uniform electric field [59,60,61].…”

Section: Cell Orientationmentioning

confidence: 99%

Computer vision guided embryo biopsy

Wang¹

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Embryo biopsy, which is a process to remove a part of materials from a cell, is a necessary step of preimplantation genetic diagnosis (PGD). The rapid advancement of the PGD technique has been calling for easy, fast and precise automated embryo biopsy techniques to improve the efficiency, the throughput, and the survival rate of the PGD. In this thesis, a computer vision guided embryo biopsy system is introduced. This research is devoted to

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Electromechanical effects on multilayered cells in nonuniform rotating fields

Cited by 13 publications

References 48 publications

Dielectric Characterization of the Yeast Cell Budding Cycle

Dielectric Characterization of the Yeast Cell Budding Cycle

Noncontact Manipulation of Intracellular Structure Based on Focused Surface Acoustic Waves

Computer vision guided embryo biopsy

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