Electron Optic Morphological and Cytochemical Evidence in Support of a ‘Cytoplasmic’ Solution for Kidney Preservation

Abstract

A complex fluid containing electrolytes, metabolic substrates, enzyme cofactors, and hydrogen acceptors in concentrations similar to those of the renal cytoplasm was successfully used in preserving kidneys up to 72 h withσut artificial perfusion. The results of electron optic investigations presented in this paper demonstrate that some changes in the renal ultrastructure and enzyme reactions occur suggesting a simple low-grade hyperhydration of the renal cells and that this fluid has no irreversible, deleterious effects on these cells.

Protective Effects of PGF_2α and PGE₂ against Immunological Actions in Long-Term Preserved Rat Hearts

“…Details of the method have been published elsewhere [50], 2 Expressed as mVal/1,000 ml, these electrolyte concentrations are equivalent to 3.33 for N a \ 2.38 for K \ 0.47 for Mg2*, 5.04 for C h and 2.38 for CHjCOO-. …”

Exceptional performance in heart preservation with an amino acid—Containing perfusion fluid

“…Electrolytes (mOsm/1,000 ml) L-alanine the end of the functional testing period in order to find more subtle evidence of cellular lesions. The technique of morphological study has been described previously [6]. Fig.…”

“…If this technique of preservation is to be applied clinically, more prolonged observation would be required to establish functional integrity of the preserved organs. It is generally felt that the advantages of orthotopic implantation more than compensate for the additional technical difficulties [6].…”

“…In a previous report [2], it was theoretically argued that the only reasonable procedure for optimal long-term preservation of tissues and organs, involving a rapid thermal exchange, consists of maintaining a functional cellular steady state during the whole period of preservation, and that this procedure requires a preservation fluid consisting of substances belonging to all the principal metabolic compartments (not only to the electrolyte compartment as Collins et al [3,4] have stated), in concentrations identical to those existing in normal cytoplasm. This theory was successfully applied to long-term hypothermic preservation of kidney [5,6]. As far as the heart is concerned, in an earlier report [7] we have shown that at deep hypothermic levels it preferentially utilizes amino acids and fatty acids instead of glucose.…”