Electron optical studies of bovine viral diarrhea-mucosal disease virus (BVDV)

Abstract

After concentration and purification steps involving ultracentrifugation, density gradient centrifugation in potassium tartrate and rate zonal centrifugation in saccharose the virus of bovine virus diarrhea-mucosal disease of cattle (strain Oregon C24 V) was studied electron microscopically. The virion consists of an envelope without projections and a nucleocapsid probably displaying a cubical symmetry. The particle has a diameter of 57 • 7 m~z the core accounting for 24 q-4m~z.The morphological relationship to other viruses is discussed. ZusammenfassungNach der Konzentration durch Ultrazentrifugierung und nach Reinigungsschritten durch Dichtezentrifugierung in Kaliumtartrat und Zonenzentrifugierung in Saccharose wurde das Virus der bovinen Virusdiarrhoe-Mucosal Disease (Stamm Oregon C24 V) elektronenoptisch dargestellt. Das Virion besteht aus einer Hfille ohne Projektionen und einem wahrscheinlich kubisch-symmetrischen Nukleokapsid. Der Durchmesser des Virion betr/~gt 57 • 7 m~z, der des Innen-kSrpers 24 =k 4 m~z.Die morphologische Verwandtschaft mit anderen Viren wird diskutiert.

Electron microscopic studies of bovine viral diarrhea virus in tissues of diseased calves and in cell cultures

“…Polymorfism of the virions, similar to what was observed in the present study has been reported for rubella virus (5,19) and lactic dehydrogenase elevating virus (1, 4,33,41). It has been argued that this pleomorfism represents artifacts caused by preparation (4,16) rather than true forms.…”

“…This has revealed a wide range in particle size, the estimations ranging from 35 to more than 100 nm (14,15,17,19,34,40). This has revealed a wide range in particle size, the estimations ranging from 35 to more than 100 nm (14,15,17,19,34,40).…”

“…This has revealed a wide range in particle size, the estimations ranging from 35 to more than 100 nm (14,15,17,19,34,40). This led to the detection of particles, 45--55 nm in diameter, within characteristic vesicular structures.…”

The Ultrastructure of Cell Cultures Infected with Border Disease and Bovine Virus Diarrhoea Viruses

“…Other studies of BVDV in thin sections have been made by Ritchie & Fernelius (1969) and Ohmann & Bloch (1982) who described BVDV virus as spherical particles approximately 50 nm in diameter. Most studies of BVDV have utilized suspensions of virus and negative staining (Hafez et al, 1968;Maess & Retzko, 1970;Horzinek et al, 1971 ;Stott et al, 1974;Chu & Zee, 1984), and have variously reported the virus as roughly spherical and from 30 to 100 nm or more in diameter.…”

Virus-like particles in bovine turbinate cells infected with bovine virus diarrhoea/mucosal disease virus

“…Examination by eteetron microscopy of negatively stained virus preparations has revealed a variety of generally undistinguished structures (4,8,9,11,16). Although these might represent enveloped togavirus particles, whose morphology is ill-defined in this type of preparation, the reported size range of 30--100 nm is large.…”