Electrophoretic coating of amphiphilic chitosan colloids on regulating cellular behaviour

Abstract

In this communication, we report a facile nanotopographical control over a stainless steel surface via an electrophoretic deposition of colloidal amphiphilic chitosan for preferential growth, proliferation or migration of vascular smooth muscle cells (VSMCs) and human umbilical vein endothelial cells (HUVECs). Atomic force microscopy revealed that the colloidal surface exhibited a deposition time-dependent nanotopographical evolution, wherein two different nanotopographic textures indexed by 'kurtosis' (R kur ) value were easily designed, which were termed as 'sharp' (i.e. high peak-to-valley texture) surface and 'flat' (i.e. low peak-to-valley texture) surface. Cellular behaviour of VSMCs and HUVECs on both surfaces demonstrated topographically dependent morphogenesis, adherent responses and biochemical properties in comparison with bare stainless steel. The formation of a biofunctionalized surface upon a facile colloidal chitosan deposition envisions the potential application towards numerous biomedical devices, and this is especially promising for cardiovascular stents wherein a new surface with optimized texture can be designed and is expected to create an advantageous environment to stimulate HUVEC growth for improved healing performance.

Nanostructured Biointerfacing of Metals with Carbon Nanotube/Chitosan Hybrids by Electrodeposition for Cell Stimulation and Therapeutics Delivery

“…In other words, the CNT-aided nanostructured surface should play a key role in the cellular anchorage, and subsequent cytoskeletal extension processes. It has also been accepted that topography, particularly nanotopology, strongly influences the morphology and orientation of living cells, through a phenomenon known as contact guidance, and through integrin-mediated intracellular tension, which would play an essential role in determining the characteristic functions of complex tissues. − …”