Electrospun aligned nanofibrous composite of MWCNT/polyurethane to enhance vascular endothelium cells proliferation and function

Meng, Jie; Han, Zhaozhao; Hua, Ke; Qi, Xiaojin; Wang, Chaoying; Xie, Sishen; Xu, Haiyan

doi:10.1002/jbm.a.32845

Cited by 60 publications

(32 citation statements)

References 54 publications

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“…The net structure of the nanofibrous membranes was scanned by scanning electron microscopy (S-4800; Hitachi Ltd., Tokyo, Japan), according to the procedures described by a previous study. 26 The mechanical characters of the PLGA and GO-PLGA membranes were measured by a mechanical test device (H5K-S; Hounsfield, Salfords, UK). The stress-strain curve of these membranes was obtained from the deformation curves at a tensile speed (0.5 mm/s).…”

Section: Characterization Of the Nanofibrous Membranesmentioning

confidence: 99%

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Su¹,

Wang²,

Jiang³

et al. 2019

IJN

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Background These normal entheses are not reestablished after repair despite significant advances in surgical techniques. There is a significant need to develop integrative biomaterials, facilitating functional tendon-to-bone integration. Materials and methods We fabricated a highly interconnective graphene oxide-doped electrospun poly(lactide-co-glycolide acid) (GO-PLGA) nanofibrous membrane by electrospinning technique and evaluated them using in vitro cell assays. Then, we established rabbit models, the PLGA and GO-PLGA nanofibrous membranes were used to augment the rotator cuff repairs. The animals were killed postoperatively, which was followed by micro-computed tomography, histological and biomechanical evaluation. Results GO was easily mixed into PLGA filament without changing the three dimensional microstructure. An in vitro evaluation demonstrated that the PLGA membranes incorporated with GO accelerated the proliferation of BMSCs and furthered the Osteogenic differentiation of BMSCs. In addition, an in vivo assessment further revealed that the local application of GO-PLGA membrane to the gap between the tendon and the bone in a rabbit model promoted the healing enthesis, increased new bone and cartilage generation, and improved collagen arrangement and biomechanical properties in comparison with repair with PLGA only. Conclusion The electrospun GO-PLGA fibrous membrane provides an effective approach for the regeneration of tendon to bone enthesis.

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Section: Characterization Of the Nanofibrous Membranesmentioning

confidence: 99%

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Su¹,

Wang²,

Jiang³

et al. 2019

IJN

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“…It has extensive potential to be used in soft tissue engineering applications such as in wound dressings and vascular grafts . Although aligned PU and PU composite (e.g., PU/carbon nanotube (CNT) and TPU/chitosan/collagen electrospun fibers have been prepared using a rotation drum as the collector, the extent of fiber orientation had a large deviation which might not have been high enough to direct cell migration. Moreover, to the best of our knowledge, no studies have been published that incorporate conductive plates to produce aligned or orthogonal TPU electrospun fibers.…”

Section: Introductionmentioning

confidence: 99%

Electrospinning of unidirectionally and orthogonally aligned thermoplastic polyurethane nanofibers: Fiber orientation and cell migration

Salick

Jing

et al. 2014

J Biomedical Materials Res

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Unidirectionally and orthogonally aligned thermoplastic polyurethane (TPU) nanofibers were electrospun using a custom-built electrospinning device. The unidirectionally aligned fibers were collected using two parallel copper plates, and the orthogonally aligned fibers were collected using two orthogonal sets of parallel copper plates with alternate negative connections. Carbon nanotubes (CNT) and polyacrylic acid (PAA) were added to modify the polymer solution. It was found that both CNT and PAA were capable of increasing solution conductivity. The TPU/PAA fiber showed the highest degree of fiber orientation with more than 90% of the fibers having an orientation angle between −10° and 10° for unidirectionally aligned fibers, and for orthogonally aligned fibers, the orientation angle of 50% fibers located between −10° and 10° and 48% fibers located between 80° and 100°. Viability assessment of 3T3 fibroblasts cultured on TPU/PAA fibers suggested that the material was cytocompatible. The cells’ orientation and migration direction closely matched the fibers’ orientation. The cell migration velocity and distance were both enhanced with the guidance of fibers compared with cells cultured on random fibers and common tissue culture plastic. Controlling cell migration velocity and directionality may provide ways to influence differentiation and gene expression and systems that would allow further exploration of wound repair and metastatic cell behavior.

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“…The results demonstrated that HUVECs cultured on CNT/PU 2:10 and 3:10 could proliferate more than other groups, indicating nontoxic nature of the used materials. Others have also reported that fabricated nanofibrous composites of CNT/PU by electrospinning could mimic the structure and function of extracellular by stimulating cell growth, proliferation, and extracellular collagen secretion (Meng et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…The F I G U R E 4 Stress-strain curves of PU and CNT/PU 2:10, 3:10, and 6:10 wt% nanocomposites. (Meng et al, 2010).…”

Section: Cytocompatibility Resultsmentioning

confidence: 99%

Development of electrically conductive hybrid nanofibers based on CNT‐polyurethane nanocomposite for cardiac tissue engineering

Shokraei

Asadpour

Shokraei

et al. 2019

Microscopy Res & Technique

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Conductive nanofibers have been considered as one of the most interesting and promising candidate scaffolds for cardiac patch applications with capability to improve cell–cell communication. Here, we successfully fabricated electroconductive nanofibrous patches by simultaneous electrospray of multiwalled carbon nanotubes (MWCNTs) on polyurethane nanofibers. A series of CNT/PU nanocomposites with different weight ratios (2:10, 3:10, and 6:10wt%) were obtained. Scanning electron microscopy, conductivity analysis, water contact angle measurements, and tensile tests were used to characterize the scaffolds. FESEM showed that CNTs were adhered on PU nanofibers and created an interconnected web‐like structures. The SEM images also revealed that the diameters of nanofibers were decreased by increasing CNTs. The electrical conductivity, tensile strength, Young's modulus, and hydrophilicity of CNT/PU nanocomposites also enhanced after adding CNTs. The scaffolds revealed suitable cytocompatibility for H9c2 cells and human umbilical vein endothelial cells (HUVECs). This study indicated that simultaneous electrospinning and electrospray can be used to fabricate conductive CNT/PUnanofibers, resulting in better cytocompatibility and improved interactions between the scaffold and cardiomyoblasts.

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Electrospun aligned nanofibrous composite of MWCNT/polyurethane to enhance vascular endothelium cells proliferation and function

Cited by 60 publications

References 54 publications

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Electrospinning of unidirectionally and orthogonally aligned thermoplastic polyurethane nanofibers: Fiber orientation and cell migration

Development of electrically conductive hybrid nanofibers based on CNT‐polyurethane nanocomposite for cardiac tissue engineering

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