Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells

Abstract

This study investigated the adhesive behaviors of normal and abnormal hematopoietic cells on nanotopographical materials. Previously, electrospun nanofiber scaffolds (NFSs) were used to capture and expand hematopoietic stem cells in vitro; here, we demonstrate that NFS could also serve as a useful bioadhesive platform for capturing functionally adherent leukemia cells. Collagen-blended poly(d,l-lactide-co-glycolide) NFS enabled more rapid and efficient capture of K562 leukemia cells than tissue culture polystyrene surfaces with up to 70% improved adhesion and shorter time. Cellular extensions, stronger adhesion, and enhanced cell-cell interactions were observed in K562 cells captured on NFS. While NFS promoted hematopoietic progenitor cell proliferation, it inhibited leukemia cell proliferation and affected cell cycle status by shifting more cells toward the G0/G1 phase. The expression of α-integrins was equally high in both captured and uncaptured leukemia cell populations demonstrating no relation to its adhesive nature. Hematopoietic morphological signatures of NFS captured cells presented no impact on cell differentiation. We conclude that electrospun NFS serves as an excellent platform not only for capturing functionally adherent leukemia cells but also for studying the impact of niche-like structure in the nanoscale.

Antibacterial Activity and Cytocompatibility of PLGA/CuO Hybrid Nanofiber Scaffolds Prepared by Electrospinning

“…Cell Adhesion. NIH3T3 cells tend to get adhered to the surface of the biocompatible materials; therefore the various cell fate processes including proliferation, migration, apoptosis, and differentiation are highly affected by cells adhesion to cell-binding epitopes in the extracellular matrix (ECM) [33]. Figure 8 depicts the FE-SEM images of the NIH3T3 cells adhered to the pristine PLGA and PLGA/CuO hybrid nanofiber scaffolds with different incubation time.…”

The hollow fiber bioreactor as a stroma‐supported, serum‐free ex vivo expansion platform for human umbilical cord blood cells

“…Many different types of materials have been tested for making 3D scaffolds to support hematopoietic cell growth. Nanostructured features such as electrospun nanofibers have also been shown to play a role in capturing and expanding hematopoietic cells in vitro [39][40][41]. We hypothesize that this unique bio-artificial architecture provided in the HFBR better mimics the cellular compartment of human bone marrow, thus stimulating the growth of HSCs.…”

“…A greater cell harvest of primitive hematopoietic population was found in the adherent population in the HFBR culture and this may be attributed to the 3D architecture that the membrane fibers and stromal cells collaboratively created (Fig. Nanostructured features such as electrospun nanofibers have also been shown to play a role in capturing and expanding hematopoietic cells in vitro [39][40][41]. In the context of 3D microenvironments, other groups have also shown that 3D architectures mimicking the osteoblast niche favored the expansion of hematopoietic stem/progenitor cells compared to 2D systems [38].…”

Fabrication and Evaluation of Multilayer Nanofiber-Hydrogel Meshes with a Controlled Release Property

“…While our long-term intent is to grow and modulate hematopoietic cells, K562 cells are a good model system for such studies. Although collagen blended poly(d,l-lactide-co-glycolide) nanofiber scaffolds used for capturing adherent K562 cells has been reported [34], there is no report on the cytotoxic evaluation of electrospun PCL nanofibers on K562 cells in the literature.…”