Electrospun poly(ε‐caprolactone)‐based skin substitutes: In vivo evaluation of wound healing and the mechanism of cell proliferation

Abstract

In the present study, we have fabricated electrospun poly(ε-caprolactone)-based membranes, characterized and studied the in vivo cell migration and proliferation and wound healing activity. Moreover, we did not seed any cells prior to the animal implantation and we could observe excellent fibroblast attachment and cell proliferation. Further full thickness excision wound on guinea pig completely healed within 35 days. We could reach in an assumption that the enhanced cell proliferation and wound healing might be due to the surface degradation of the polymer under physiological conditions and the formation of functional groups like hydroxyl and carboxyl groups that promoted cell proliferation in a cell adhesion protein mediated mechanism. This study is a novel tissue engineering concept for the reconstruction of a damaged tissue without the in vitro cell seeding and proliferation prior to the in vivo implantation.

Enhanced osteogenesis of mesenchymal stem cells on electrospun cellulose nanocrystals/poly(ε-caprolactone) nanofibers on graphene oxide substrates

“…The surface morphologies of the fabricated are affected by several factors such as nature of the solvent, concentration, and viscosity of the solution, distance, charge density and applied voltage. [18][19][20] The fabricated scaffolds have smooth morphology without noticeable beads and thinner diameter was observed in PC scaffolds as compared to pure PCL scaffolds. A similar result was also observed by Shi et al in the presence of CNCs with the poly(lactic acid) polymer matrix.…”

“…14 Polymer/CNCs composites have been studied from the last several decades in tissue engineering applications. [15][16][17][18][19][20][21][22][23][24] Besides, it has been found that the external stimuli including the electrical eld have wide effects on cell proliferation in the presence of CNCs. 25 It was observed that CNCs have low electroactive materials, and this property can be tuned by using highly conductive materials such as graphene or carbon nanotube with CNCs.…”

Beyond 2D: 3D bioprinting for skin regeneration

“…7 Although many biomaterials are widely used in tissue engineering, such as PLA and PCL, cell compatibility is essential for the selection of bioink for cell encapsulation and bioprinting. 35 For instance, PLA with a relatively high processing (melting) temperature is not suitable for bioprinting. 6 Based on this and previous studies, hydrogels 36 ; natural biopolymers such as alginate, chitosan, gelatin, etc.…”

Microbial Interactions with Nanostructures and their Importance for the Development of Electrospun Nanofibrous Materials used in Regenerative Medicine and Filtration

“…In another aspect of regenerative medicine, wound healing, electrospinning is now an established technique for the production of wound dressings for the treatment of chronic wounds [6,[29][30][31][32][33][34][35]. Many modern wound dressings are no longer only a barrier to protect a wound from infection but they are also expected to enhance the healing of the wound [36].…”