Electrospun Polymer-Fungicide Nanocomposites for Grapevine Protection

Nachev, Nasko; Spasova, Mariya; Tsekova, Petya; Manolova, Nevena; Rashkov, Iliya; Naydenov, Mladen

doi:10.3390/polym13213673

Cited by 5 publications

(6 citation statements)

References 32 publications

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“…The obtained results from the CQ release studies showed that the CQ release from the fibrous materials was assisted by the presence of PVP in the fibrous materials or on their surface. These results are consistent with our previous findings on an increase in the rate of release of 8-hydroxyquinoline derivatives from other fibrous systems upon incorporation of a water-soluble polymer [ 21 , 22 ]. In the present study, using one-pot electrospinning or electrospinning in conjunction with electrospraying, fibrous materials of diverse design and with different CQ release profiles were fabricated.…”

Section: Resultssupporting

confidence: 93%

“…They also have low toxicity to humans. Some of us have reported the successful encapsulation of 8-hydroxyquinolines in electrospun materials based on cellulose derivative and poly(ethylene glycol) [ 21 ] and polylactide [ 22 ] to obtain materials that provide effective protection by impeding the entrance and growth of fungal spores on vine plants.…”

Section: Introductionmentioning

confidence: 99%

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Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca

et al. 2022

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Esca is a grapevine disease known for centuries which pertains to the group of so-called vine trunk diseases. Phaeomoniella chlamydospora (P. chlamydospora) and Phaeoacremonium aleophilum (P. aleophilum) are the two main fungal pathogens associated with esca. Novel fibrous materials with antifungal properties based on poly(3-hydroxybutyrate) (PHB), polyvinylpyrrolidone (PVP) and 5-chloro-7-iodo-8-hydroxyquinoline (clioquinol, CQ) were developed. One-pot electrospinning (“in” strategy) or electrospinning in conjunction with electrospraying (“on” strategy) were applied to obtain the materials. The materials’ morphology and their surface chemical composition were examined using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). CQ incorporated in the bulk of the fibers or in PVP particles deposited on the fibers was in the amorphous phase, which was confirmed by differential scanning calorimetry (DSC) and X-ray diffraction analysis (XRD). The in vitro release of CQ depended on the composition of the electrospun materials and on their design. The performed microbiological screening revealed that, unlike the non-loaded mats, the fibrous mats loaded with CQ were effective in inhibiting the growth of the pathogenic P. chlamydospora and P. aleophilum fungi. Therefore, the created materials are promising as active dressings for grapevine protection against esca.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca

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“…Two types of 8-hydroxyquinoline derivatives were used—5-chloro-8-hydroxyquinoline and potassium 5-nitro-8-hydroxyquinoline. They were selected because of their low MICs against P. chlamydospora and P. aleophilum fungi [ 98 ].…”

Section: Electrospun Fibrous Materials With Antifungal Activity Again...mentioning

confidence: 99%

“… Representative SEM images of fibers of electrospun fibrous materials of ( a ) PLLA, ( b ) PLLA/5-Cl8Q, and ( c ) PLLA/K5N8Q; magnification ×2500 [ 98 ]. …”

Section: Figurementioning

confidence: 99%

Electrospun Polymer Materials with Fungicidal Activity: A Review

et al. 2022

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In recent years, there has been special interest in innovative technologies such as polymer melt or solution electrospinning, electrospraying, centrifugal electrospinning, coaxial electrospinning, and others. Applying these electrokinetic methods, micro- or nanofibrous materials with high specific surface area, high porosity, and various designs for diverse applications could be created. By using these techniques it is possible to obtain fibrous materials from both synthetic and natural biocompatible and biodegradable polymers, harmless to the environment. Incorporation of low-molecular substances with biological activity (e.g., antimicrobial, antifungal) is easily feasible. Moreover, biocontrol agents, able to suppress the development and growth of plant pathogens, have been embedded in the fibrous materials as well. The application of such nanotechnologies for the creation of plant protection products is an extremely promising new direction. This review emphasizes the recent progress in the development of electrospun fungicidal dressings and their potential to be applied in modern agriculture.

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“…In our previous studies, we prepared fibrous materials based on biodegradable and biocompatible polymers loaded with an antifungal agent for active protection against spore penetration and plant infection in vineyards [31][32][33]. The incorporation of diverse 8-hydroxyquinoline derivatives into the fibers imparted to them considerable antifungal activity that acted against P. chlamydospora and P. aleophilum.…”

Section: Introductionmentioning

confidence: 99%

Eco-Friendly Hybrid PLLA/Chitosan/Trichoderma asperellum Nanomaterials as Biocontrol Dressings against Esca Disease in Grapevines

et al. 2022

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Fungi constitute the largest number of plant pathogens and are responsible for a range of serious plant diseases. Phaeomoniella chlamydospora (P. chlamydospora) and Phaeoacremonium aleophilum (P. aleophilum) are the main fungal pathogens causing esca disease in grapevines. On the other hand, there are beneficial microorganisms such as Trichoderma spp., which are able to control the growth of many phytopathogens. In the present study, innovative, eco-friendly hybrid nanomaterials were created by electrospinning PLLA, followed by the formation of a film of chitosan/Trichoderma asperellum (T. asperellum) spores on the fibers. The polymer carrier used in this study plays an active role in ensuring the viability of the biological agent during storage and, when placed in contact with moisture, ensures the agent’s normal development. Oligochitosan, as well as low molecular weight and high molecular weight chitosan, were used. The effects of chitosan molecular weight on the dynamic viscosity of chitosan solutions, film formation, mechanical properties, spore incorporation and growth were studied. The morphology of the prepared nanomaterials, and the presence of a film based on the formation of chitosan/T. asperellum spores on the PLLA fibers, were examined using scanning electron microscopy (SEM). The surface chemical compositions of the fibrous materials were studied using attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). The mechanical properties of the obtained materials were also tested. The microbiological screening that was performed revealed that the eco-friendly hybrid nanomaterials incorporated with the beneficial microorganism, T. asperellum, to hamper the growth of the pathogenic P. chlamydospora and P. aleophilum fungi. The suppression rate depended on the viscosity of the chitosan solution used for the film formation. The use of oligochitosan resulted in the most effective infection of the material with T. asperellum spores. The environmentally friendly hybrid nanomaterials obtained in this study—in which the bioagent was embedded—are promising bioactive dressings for protecting grapevines against esca disease.

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Electrospun Polymer-Fungicide Nanocomposites for Grapevine Protection

Cited by 5 publications

References 32 publications

Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca

Electrospun 5-Chloro-7-iodo-8-hydroxyquinoline (Clioquinol)-Containing Poly(3-hydroxybutyrate)/Polyvinylpyrrolidone Antifungal Materials Prospective as Active Dressings against Esca

Electrospun Polymer Materials with Fungicidal Activity: A Review

Eco-Friendly Hybrid PLLA/Chitosan/Trichoderma asperellum Nanomaterials as Biocontrol Dressings against Esca Disease in Grapevines

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