Electrospun polyvinyl alcohol–collagen–hydroxyapatite nanofibers: a biomimetic extracellular matrix for osteoblastic cells

Song, Wei; Markel, David C.; Wang, Sunxi; Shi, Tong; Mao, Guangzhao; Ren, Weiping

doi:10.1088/0957-4484/23/11/115101

Cited by 85 publications

(64 citation statements)

References 62 publications

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“…Until now, all collagen-HA composites obtained by electro-spinning were prepared from a mixture of collagen and hydroxyapatite. As a consequence, the composite surface is covered with collagen or HA, preventing direct cell/protein contact with both organic and inorganic components [15,33,43,44]. The cross-linking procedure did not affect the morphological arrangement of the electrospun meshes.…”

Section: In Vitro Cell Culture Studiesmentioning

confidence: 99%

A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration

et al. 2014

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This work aims to design a synthetic construct that mimics the natural bone extracellular matrix through innovative approaches based on simultaneous type I collagen electrospinning and nanophased hydroxyapatite (nanoHA) electrospraying using non-denaturating conditions and non-toxic reagents. The morphological results, assessed using scanning electron microscopy and atomic force microscopy (AFM), showed a mesh of collagen nanofibers embedded with crystals of HA with fiber diameters within the nanometer range (30 nm), thus significantly lower than those reported in the literature, over 200 nm. The mechanical properties, assessed by nanoindentation using AFM, exhibited elastic moduli between 0.3 and 2 GPa. Fourier transformed infrared spectrometry confirmed the collagenous integrity as well as the presence of nanoHA in the composite. The network architecture allows cell access to both collagen nanofibers and HA crystals as in the natural bone environment. The inclusion of nanoHA agglomerates by electrospraying in type I collagen nanofibers improved the adhesion and metabolic activity of MC3T3-E1 osteoblasts. This new nanostructured collagennanoHA composite holds great potential for healing bone defects or as a functional membrane for guided bone tissue regeneration and in treating bone diseases.

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Section: In Vitro Cell Culture Studiesmentioning

confidence: 99%

A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration

et al. 2014

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“…Interestingly, the increase on HAp content stimulated the proliferation of osteogenic cells due to different effects: (a) upregulated expression of genes associated to gene expression [62]; (b) mitogenic effect of HAp crystals [63]; and (c) reinforcement effect that improves mechanical properties and lead materials with better capacities to support cell colonization [64]. The improvement of osteoconductivity is clear for example for chitosan based scaffolds [65].…”

Section: Electrospun Scaffolds Incorporating Hap Nanoparticlesmentioning

confidence: 99%

Biodegradable and Biocompatible Systems Based on Hydroxyapatite Nanoparticles

et al. 2017

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Abstract:Composites of hydroxyapatite (HAp) are widely employed in biomedical applications due to their biocompatibility, bioactivity and osteoconductivity properties. In fact, the development of industrially scalable hybrids at low cost and high efficiency has a great impact, for example, on bone tissue engineering applications and even as drug delivery systems. New nanocomposites constituted by HAp nanoparticles and synthetic or natural polymers with biodegradable and biocompatible characteristics have constantly been developed and extensive works have been published concerning their applications. The present review is mainly focused on both the capability of HAp nanoparticles to encapsulate diverse compounds as well as the preparation methods of scaffolds incorporating HAp. Attention has also been paid to the recent developments on antimicrobial scaffolds, bioactive membranes, magnetic scaffolds, in vivo imaging systems, hydrogels and coatings that made use of HAp nanoparticles.

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“…Synthetic polymers include a broad spectrum of biostable polymers, for example polyethylene terephthalate [9], polytetrafluoroethylene [22] and polyurethane, suitable for fabrication of vascular grafts [23], and biodegradable polymers, for example polylactides (PLA) [24,25] and their copolymers with polyglycolides (PLGA) [1,26], polycaprolactone (PCL) [2,4,6], poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) [27], polydioxanone [5], polyvinylalcohol (PVA) [28,29], and synthetic peptides [7,30].…”

Section: Introductionmentioning

confidence: 99%

“…This is feasible by addition of inorganic nanoparticles into nanofibers, such as ceramic nanoparticles, for example hydroxyapatite [16,27,28,30], tricalcium phosphate [63,66], calcium oxide [67], or calcium silicate [24]; metal-based nanoparticles, for example gold nanoparticles [29], ferro- [62], or nanodiamonds [68]. These nanoparticles not only reinforce the polymeric nanofibers but also enhance their bioactivity in terms of increased cell adhesion, growth, osteogenic cell differentiation, bone matrix mineralization and antimicrobial activity.…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

Bačáková¹,

Bačáková²,

Pajorová³

et al. 2016

Nanofiber Research - Reaching New Heights

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Nanofibers are promising cell carriers for tissue engineering of a variety of tissues and organs in the human organism. They have been experimentally used for reconstruction of tissues of cardiovascular, respiratory, digestive, urinary, nervous and musculoskeletal systems. Nanofibers are also promising for drug and gene delivery, construction of biosensors and biostimulators, and wound dressings. Nanofibers can be created from a wide range of natural polymers or synthetic biostable and biodegradable polymers. For hard tissue engineering, polymeric nanofibers can be reinforced with various ceramic, metal-based or carbon-based nanoparticles, or created directly from hard materials. The nanofibrous scaffolds can be loaded with various bioactive molecules, such as growth, differentiation and angiogenic factors, or funcionalized with ligands for the cell adhesion receptors. This review also includes our experience in skin tissue engineering using nanofibers fabricated from polycaprolactone and its copolymer with polylactide, cellulose acetate, and particularly from polylactide nanofibers modified by plasma activation and fibrin coating. In addition, we studied the interaction of human bone-derived cells with nanofibrous scaffolds loaded with hydroxyapatite or diamond nanoparticles. We also created novel nanofibers based on diamond deposition on a SiO 2 template, and tested their effects on the adhesion, viability and growth of human vascular endothelial cells.

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Electrospun polyvinyl alcohol–collagen–hydroxyapatite nanofibers: a biomimetic extracellular matrix for osteoblastic cells

Cited by 85 publications

References 62 publications

A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration

A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration

Biodegradable and Biocompatible Systems Based on Hydroxyapatite Nanoparticles

Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

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