Electrospun scaffolds of polylactide with a different enantiomeric content and loaded with anti-inflammatory and antibacterial drugs

Abstract

Polylactide (PLA) electrospun microfibers were prepared and loaded with triclosan (TCS), ketoprofen (KTP), or their combination to obtain multifunctional scaffolds with bactericide and anti-inflammatory properties. Continuous and porous fibers with diameters in the micrometer scale and a unimodal distribution were successfully attained using a dual-electrospinning technique. Dual drug-loaded scaffolds showed a peculiar release that was in contrast to the single drug-loaded systems, which suggested the establishment of intermolecular interactions that delayed TCS and KTP release. Antimicrobial activity of all TCS-loaded electrospun scaffolds was demonstrated against E. coli and M. luteus bacteria; and furthermore, KTP-loaded samples slightly showed bactericide activity. Biocompatibility of scaffolds was evaluated by adhesion and proliferation assays, and interestingly, the dual drug-load systems were able to support high TCS doses without adverse effects.

Incorporation of Chloramphenicol Loaded Hydroxyapatite Nanoparticles into Polylactide

“…Conditions for the electrospinning of PLA have been optimized in earlier works [32,33,34]. Thus, a chloroform-acetone mixture (2:1 v/v) with a polymer concentration of 8 wt-% was adequate to get fibers with diameters in the nano/micrometric range.…”

Chloramphenicol loaded polylactide melt electrospun scaffolds for biomedical applications

“…While a very slight burst effect is observed for SES-samples (i.e., 5 wt-% and 2 wt-% of drug is released in the first 5 minutes for PLA_CAM 10 and PLA_CAM 5), MES-samples seem to start the release only after a lag time. This difference is probably due to the lower diameter of SES-samples, which also influences on the subsequent release behaviour, and their lower crystallinity (between 2% and 7% for pure PLA with respect to the 14% of melt electrospun PLA; see Table 1 and Del Valle et al, 2010 andLlorens et al, 2015). A lag time of ~3600 s (1h) can be observed for the melt electrospun scaffolds (see the semilogarithmic plot in the inset of Figure 5).…”

Supercritical CO2 deposition and foaming process for fabrication of biopolyester–ZnO bone scaffolds

“…Commonly methods used to generate highly porous PLA‐based polymer or composite materials for tissue engineering such as solvent casting/salt leaching, fiber forming and 3D printing require use of liquid solvents and/or a drying step (i.e., freeze drying). Introduction of thermal gradients during drying (i.e., freeze drying process) can result in undesirable anisotropy of pore size and alignment.…”