Electrostatic deposition of polycations and polyanions onto cysteine monolayers

Sanders, Wesley C.; Anderson, Mark R.

doi:10.1016/j.jcis.2008.12.010

Cited by 9 publications

(10 citation statements)

References 24 publications

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“…The isoelectric points (pI) were pI ¼ 5.2 and 3.9 for Cys-Au and GSH-Au, respectively. The pI value of Cys-Au obtained is comparable with that in the literature 20 but significantly higher than the reported value of 4.1 for Cys on silver nanoparticles. 21 At low pH, both carboxyl and amine groups are protonated leading to the net positive charge at surfaces, whereas deprotonation at high pH rendered the surfaces negatively charged.…”

Section: A Formation and Characterization Of Samssupporting

confidence: 84%

“…Among these compounds, Cys is the most popular surface ligand for the preparation of biofunctional surfaces, as a model system for the investigation of organic-inorganic interfaces. [15][16][17][18][19][20][21][22][23][24] A variety of techniques, including x-ray photon electron spectroscopy (XPS), ion mass spectroscopy, scanning tunneling microscopy, surfaceenhanced Raman scattering, and surface-enhanced second harmonic generation, have been applied to scrutinize Cys on metals at a molecular level. [15][16][17][22][23][24] The ability of Cysmodified surfaces to repel protein adsorption was also well documented.…”

Section: Introductionmentioning

confidence: 99%

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Natural zwitterionic organosulfurs as surface ligands for antifouling and responsive properties

et al. 2014

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Natural sulfur-containing zwitterionic compounds, l-cysteine (Cys), l-methionine, and glutathionine (GSH), have been employed as surface ligands to prevent protein nonspecific adsorption on planar substrates. These organosulfur compounds form self-assembled monolayers (SAMs) on gold substrates by gold–sulfur interaction. The chemical elements of SAMs were confirmed using x-ray photoelectron spectroscopy. The surface wetting tests for SAMs show that films prepared from Cys and GSH exhibited super-hydrophilicity (contact angles of θ = ∼5°) due to their high coverage and strong hydration via ionic solvation and formation of hydrogen bonding. Quartz crystal microbalance with dissipation sensor was used to quantitatively and qualitatively monitor the adsorption of bovine serum albumin (BSA) from buffer onto these SAMs. It was found that the GSH film enables the resistance of BSA adsorption to the best extent at a physiological pH. Moreover, the surface charges of modified substrates were modulated by varying the pH value to control BSA adsorption. The effect of electrostatic repulsion on the antifouling behavior becomes prominent at a pH where the protein and the surface carry same charges. Consolidating the BSA adsorption measurements at different pH values, the antifouling properties of GSH-modified Au should be attributed to prevention of entropy gain and enthalpy loss, making BSA adsorption energetically unfavorable. It is believed that the surface modification with natural organosulfur ligands holds great potential in improving the biocompatibility of medical devices and in offering intelligent biointerfaces in response to environmental stimuli.

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Section: A Formation and Characterization Of Samssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Natural zwitterionic organosulfurs as surface ligands for antifouling and responsive properties

et al. 2014

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“…We previously demonstrated that altering the net charge of the cysteine monolayer by adjusting the bulk solution pH in the absence of applied potential, the electrostatic deposition of either PDDA (when using basic pH) or PSS (when using acidic pH) could be accomplished [22]. Others have also shown that changes to bulk pH create electrostatic interactions at an ionizable interface capable of driving the deposition of polyelectrolytes [8,16].…”

Section: Resultsmentioning

confidence: 99%

“…the permeability of electroactive species to the substrate surface) of the modified interface. Our interest is in creating a modified interface and then manipulating the properties of the interface so that the structure can be subsequently modified in a controlled, reproducible fashion [21,22]. We previously demonstrated that potential applied to a substrate modified with a 3-mercaptopropionic acid monolayer could be used to modulate the properties of the modified interface to drive the electrostatic assembly of polycationic poly(diallydimethyl ammonium chloride), PDDA, onto the modified substrate [21].…”

Section: Introductionmentioning

confidence: 99%

“…We recently demonstrated that this property could be leveraged to electrostatically deposit either polycationic or polyanionic polymers onto a cysteine monolayer by adjusting the bulk solution pH [22]. In this manuscript, we explore using applied potential to drive the adsorption of either polycationic PDDA or polyanionic poly(sodium styrenesulfonate), PSS, onto a substrate modified with cysteine.…”

Section: Introductionmentioning

confidence: 99%

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Potential driven deposition of polyelectrolytes onto the surface of cysteine monolayers assembled on gold

Sanders

Anderson

2010

Journal of Colloid and Interface Science

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New biomaterial based on cotton with incorporated Biomolecules

Gomes

Mano

Queiroz

et al. 2014

J of Applied Polymer Sci

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The aim of this study was to investigate a method of embedding l‐cysteine (l‐cys), an antimicrobial agent, between layers of chitosan (CH) and sodium alginate (ALG) onto cotton samples obtained via a layer‐by‐layer electrostatic deposition technique via several embedding methods. The results show that the best way to incorporate l‐cys into the layers was the one that used the property of gelling ALG. To monitor the l‐cys embedding into the CH/ALG multilayer film, different methods were used: energy‐dispersive X‐ray spectrometry analysis to assess the presence of sulfur on the sample, Ellman's reagent method to analyze l‐cys release from the sample, and attenuated total reflectance (ATR) Fourier transform infrared spectroscopy (FTIR) to compare the ATR–FTIR spectra of the pure l‐cys and l‐cys embedded in the CH/ALG multilayer film to study the interaction between the l‐cys and the CH/ALG multilayer films. Functionalized CH/ALG cotton samples were also investigated for their antibacterial properties toward Staphylococcus aureus and Klebsiella pneumonia with the Japanese Industrial Standard method JIS L 1902:2002, and the results show an enhancement of the antibacterial effect due to the presence of l‐cys. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40519.

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Electrostatic deposition of polycations and polyanions onto cysteine monolayers

Cited by 9 publications

References 24 publications

Natural zwitterionic organosulfurs as surface ligands for antifouling and responsive properties

Natural zwitterionic organosulfurs as surface ligands for antifouling and responsive properties

Potential driven deposition of polyelectrolytes onto the surface of cysteine monolayers assembled on gold

New biomaterial based on cotton with incorporated Biomolecules

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