Elektroforesis: Analisis Elektronika Terhadap Biokimia Genetika

Harahap, Muhammad Ridwan

doi:10.22373/crc.v2i1.3248

Cited by 10 publications

(9 citation statements)

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“…As an electrolyte medium of electrifying, gel agarose serves as an independent media to maintain the conditions of sample similarities during the separation process. A UV light visualization was run electrophoresis with 50 V electric tension for 1 hour and 20 minutes (Harahap, 2018). Electrophoresis readings were made on a UV-Trans illuminator or gel doc from the sample by comparing the tape from the piece with a known scale called the marker.…”

Section: Resultsmentioning

confidence: 99%

Analysis of plasmid profiles of Escherichia coli bacteria and their resistance to several antibiotics

Nurdevi¹,

Marpaung²

2022

Pharmaciana

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Escherichia coli is a bacteria belonging to the family Enterobacteriaceae. Escherichia coli is a harmful gram bacterium living in the digestive tract. These bacteria also live in water and soil, commonly referred to as coliform. This research aims to analyze the plasmid profile of Escherichia coli bacteria and its resistance to some antibiotics. To explore the plasmid profiles of Escherichia coli in a recent research electrophoresis gel, the antibiotic used in its resistance tests is the Amoxicillin, Tetracycline antibiotic, Ciprofloxacin, Sulfamethoxazole, and Streptomycin antibiotic. The electrophoresis results in a difference in the number of plasmid profiles with 1 to 2 ribbons of plasmid DNA different sizes from ˜1500 bp to ˜2300 bp. Antibiotic resistance tests have occurred in Amoxicillin, Ciprofloxacin, and some resistance samples to the Streptomycin antibiotic.

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Section: Resultsmentioning

confidence: 99%

Analysis of plasmid profiles of Escherichia coli bacteria and their resistance to several antibiotics

Nurdevi¹,

Marpaung²

2022

Pharmaciana

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“…While the DNA purity value above 2.0 indicates that there are still contaminants in the form of RNA. This could be due to not adding ribonuclease (Harahap, 2018). The next step is to mix the isolated DNA template with PCR components, namely GoTaq Green Master Mix reagent, forward primer, reverse primer, Nuclease Free Water and DNA template.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Pork DNA in Processed Meat by Polymerase Chain Reaction

Mustika¹,

Misfadhila²,

Rusdi³

et al. 2022

IJPSM

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Meatballs and kebabs are processed meat in great demand by the public, made from beef, chicken, or lamb. PCR is an enzymatic method for amplifying DNA in vitro. This study aims to determine whether pork DNA in meatballs and kebabs is analyzed using the PCR method. Sampling was conducted at three meatball outlets and three kebab outlets selected by simple random sampling. The research phase includes the isolation and purification of DNA samples and positive control (pork) using cytochrome b primer and amplification using electrophoresis. The results showed that from the three samples of meatballs, all were positive for pork DNA and from the three samples of kebabs, there was one positive sample containing pork DNA marked with the same band as the positive control (pork) at 132 bp. Therefore, it can be concluded from the six samples of processed meat examined that four samples were positive for pork DNA.

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“…30 to 60 seconds [15]. The electrophoresis process was carried out by preparing amplification products and agarose gel as a separating medium [16]. Electrophoresis is a technique for separating charged DNA molecules that aims to determine DNA quality from amplification products [8].…”

Section: Dna Isolation Amplification and Electrophoresismentioning

confidence: 99%

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

Bahri

Hikmah²,

Fadli³

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Hammerhead sharks are particularly vulnerable to fishing because of their slow growth rate and reproductive constraints. This type of sharks needs special attention due to its conservation status. This research was aimed to describe the relationships of the hammerhead sharks from West Aceh waters with the hammerhead sharks from other places. Samples of the shark were collected from Ujong Baroh Fish Port, West Aceh. The samples were analysed at the Laboratory of Department of Marine Science and Fisheries, Syiah Kuala University. The laboratory activity was started with extraction of DNA enhanced by Polymerase Chain Reaction method and visualized by an electrolytical. Analysis using BLAST showed that the first sample obtained 94%-95% cover query value and produced a 683 base pair length while the second sample obtained 98% per sample for a cover query value and produced a 658 base pair length. The average nucleotide values of the hammerhead shark were T=32.8, C=26.6, A=25.7 and G=14.9. The phylogenetic tree shows that the hammerhead sharks from the study area were more similar to the hammerhead sharks from Australia and Malaysia. Overall, based on the nucleotide composition, phylogenetic tree and genetic distance, the hammerhead sharks from the study area are unique compared to the populations from the other places.

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Elektroforesis: Analisis Elektronika Terhadap Biokimia Genetika

Cited by 10 publications

References 0 publications

Analysis of plasmid profiles of Escherichia coli bacteria and their resistance to several antibiotics

Analysis of plasmid profiles of Escherichia coli bacteria and their resistance to several antibiotics

Analysis of Pork DNA in Processed Meat by Polymerase Chain Reaction

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

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